LOCUS AK300814 2005 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56156 complete cds, highly similar to
Male-specific lethal 3-like 1.
ACCESSION AK300814
VERSION AK300814.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2005)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2005
/cell_line="SK-N-SH"
/cell_type="neuroblastoma"
/clone="SKNSH2004979"
/clone_lib="SKNSH2"
/db_xref="H-InvDB:HIT000495438"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 100..1629
/codon_start=1
/note="highly similar to Male-specific lethal 3-like 1"
/protein_id="BAG62470.1"
/transl_table=1
/translation="MSPSVRPGAGWAPVGRPGRPIQIVDVIVGKDEKGRKIPEYLIHF
NGWNRSWDRWAAEDHVLRDTDENRRLQRKLARKAVARLRSTGRKKKRCRLPGVDSVLK
GLPTEEKDENDENSLSSSSDCSENKDEEISEESDIEEKTEVKEEPELQTRREMEERTI
TIEIPEVLKKQLEDDCYYINRRKRLVKLPCQTNIITILESYVKHFAINAAFSANERPR
HHHVMPHANMNVHYIPAEKNVDLCKEMVDGLRITFDYTLPLVLLYPYEQAQYKKVTSS
KFFLPIKESATSTNRSQEELSPSPPLLNPSTPQSTESQPTTGEPATPKRRKAEPEALQ
SLRRSTRHSANCDRLSESSASPQPKRRQQDTSASMPKLFLHLEKKTPVHSRSSSPIPL
TPSKEGSAVFAGFEGRRTNEINEVLSWKLVPDNYPPGDQPPPPSYIYGAQHLLRLFVK
LPEILGKMSFSEKNLKALLKHFDLFLRFLAEYHDDFFPESAYVAACEAHYSTKNPRAI
Y"
BASE COUNT 568 a 451 c 481 g 505 t
ORIGIN
1 gctttgtcgc gttaccgggg ctaccgtttg cgcccccgac tgcaacggtg gccgctgagg
61 gaggaggctt ctcgaatacg gtttctgtct tcgcgttaaa tgtctccttc tgtgcggcct
121 ggtgccgggt gggctcctgt gggacgccct ggccgtccga tccagattgt tgatgttatt
181 gttgggaaag acgaaaaagg cagaaagatc ccagaatatc taatccattt taatggttgg
241 aacagaagct gggatagatg ggcagcagaa gatcatgtgc ttcgtgatac cgatgaaaat
301 cgtagattac agcgtaaatt ggcaagaaaa gctgtagctc gcctgaggag cacaggaaga
361 aagaagaagc gctgcaggtt gcctggtgtg gactctgtct taaaaggcct ccccactgaa
421 gaaaaagatg aaaatgatga aaactcatta agcagttcct ctgactgtag tgaaaacaag
481 gatgaagaaa taagtgaaga aagtgatatt gaagaaaaga ctgaagtgaa agaagaacca
541 gagcttcaaa caagaaggga aatggaagaa agaacaataa ctatagaaat ccctgaagtt
601 ctgaagaagc agctggagga tgattgttac tacattaaca ggaggaaacg gttagtgaaa
661 cttccatgcc agaccaacat cataacgatt ttggaatcct atgtgaagca ttttgctatc
721 aatgcagcct tttcagccaa tgagaggcct cgtcaccatc acgttatgcc acatgccaac
781 atgaacgtgc attatatccc agcagaaaag aatgttgacc tttgtaagga gatggtggat
841 ggattaagaa taacctttga ttacactctc ccgttggttt tactctatcc atatgaacaa
901 gctcagtata aaaaggtgac ttcgtctaaa ttttttcttc caattaagga aagtgccaca
961 agcactaaca ggagccagga ggaactctct cccagtccgc ctttgttgaa tccatccacg
1021 ccacagtcca cagagagtca gccgaccacc ggtgaaccag ccacccccaa aaggcgcaaa
1081 gctgagccag aagcattgca gtctctgagg cggtccacgc gccacagtgc caactgtgac
1141 aggctttctg agagcagcgc ttcacctcag cccaagcgcc ggcagcagga cacatccgcc
1201 agcatgccca agctcttcct gcacctggaa aagaagacac ctgtgcatag cagatcatct
1261 tcacctattc ctctgactcc tagcaaggaa gggagtgctg tgtttgctgg ctttgaaggg
1321 agaagaacta atgaaataaa cgaggtcctc tcctggaagc ttgtgcctga caattacccc
1381 ccaggtgacc agccgcctcc accctcttac atttatgggg cacaacattt gctgcgattg
1441 tttgtgaaac ttccagaaat ccttggaaag atgtcctttt ctgagaagaa tctgaaggct
1501 ttattgaagc actttgatct ctttttgagg tttttagcag aataccacga tgacttcttc
1561 ccagagtcgg cttatgtcgc tgcctgtgag gcacattaca gcaccaagaa cccccgggca
1621 atttattaaa atgttgttgg ttctgtaaga gcaactgctc tgtctagttt ggcgctctgg
1681 gttccaggtg aataactaac aaggtggtgg gtctttaccc acagcgcaaa cacaatgccc
1741 accttggggc tctgttgttt gagttgccca catactgcag ttattctgtt aggaatgatt
1801 ccctgggtgc ctgaaagtgc tctgacacga cacttgttac tttgcaggcc atctgtgatg
1861 gcaaggaaaa agcaactatg ttcacagtga aatattcgtg gaataggtta ggccatttca
1921 gtagacattg cagttagtta gcaagaacca cattgtctct ttatttgtta gcattaaaca
1981 aatttttttt tgcaaattgg tttta
//