LOCUS AK300806 2222 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53207 complete cds, highly similar to Homo
sapiens fibulin 1 (FBLN1), transcript variant C, mRNA.
ACCESSION AK300806
VERSION AK300806.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2222)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2222
/cell_line="SK-N-SH"
/cell_type="neuroblastoma"
/clone="SKNSH2002900"
/clone_lib="SKNSH2"
/db_xref="H-InvDB:HIT000495430"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 210..2135
/codon_start=1
/note="highly similar to Homo sapiens fibulin 1 (FBLN1),
transcript variant C, mRNA"
/protein_id="BAG62463.1"
/transl_table=1
/translation="MATHQKDCSLPYATESKECRMVQEQCCHSQLEELHCATGISLAN
EQDRCATPHGDNASLEATFVKRCCHCCLLGRAAQAQGQSCEYSLMVGYQCGQVFRACC
VKSQETGDLDVGGLQETDKIIEVEEEQEDPYLNDRCRGGGPCKQQCRDTGDEVVCSCF
VGYQLLSDGVSCEDVNECITGSHSCRLGESCINTVGSFRCQRDSSCGTGYELTEDNSC
KDIDECESGIHNCLPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAP
CPIGHTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECAPPAEPCGKGHRCVNS
PGSFRCECKTGYYFDGISRMCVDVNECQRYPGRLCGHKCENTLGSYLCSCSVGFRLSV
DGRSCEDINECSSSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGH
ICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGGFR
CLAFECPENYRRSAATRCERLPCHENRECSKLPLRITYYHLSFPTNIQAPAVVFRMGP
SSAVPGDSMQLAITGGNEEGFFTTRKVSPHSGVVALTKPVPEPRDLLLTVKMDLSRHG
TVSSFVAKLFIFVSAEL"
BASE COUNT 423 a 690 c 667 g 442 t
ORIGIN
1 gttggctgcc gaggctcggc cggagcgtgg agcccgcgcc gctgccccag gaccgcgccc
61 gcgcctttgt ccgccgccgc ccaccgcccg tcgcccgccg cccatggagc gcgccgcttc
121 cgctgctgct gctcggcggc cttgcgctgc tggcggccgg agtggacgcg gatgtcctcc
181 tggaggcctg ctgtgcggac ggacaccgga tggccactca tcagaaggac tgctcgctgc
241 catatgctac ggaatccaaa gaatgcagga tggtgcagga gcagtgctgc cacagccagc
301 tggaggagct gcactgtgcc acgggcatca gcctggccaa cgagcaggac cgctgtgcca
361 cgccccacgg tgacaacgcc agcctggagg ccacatttgt gaagaggtgc tgccattgct
421 gtctgctggg gagggcggcc caggcccagg gccagagctg cgagtacagc ctcatggttg
481 gctaccagtg tggacaggtc ttccgggcat gctgtgtcaa gagccaggag accggagatt
541 tggatgtcgg gggcctccaa gaaacggata agatcattga ggttgaggag gaacaagagg
601 acccatatct gaatgaccgc tgccgaggag gcgggccctg caagcagcag tgccgagaca
661 cgggtgacga ggtggtctgc tcctgcttcg tgggctacca gctgctgtct gatggtgtct
721 cctgtgaaga tgtcaatgaa tgcatcacgg gcagccacag ctgccggctt ggagaatcct
781 gcatcaacac agtgggctct ttccgctgcc agcgggacag cagctgcggg actggctatg
841 agctcacaga ggacaatagc tgcaaagata ttgacgagtg tgagagtggt attcataact
901 gcctccccga ttttatctgt cagaatactc tgggatcctt ccgctgccga cccaagctac
961 agtgcaagag tggctttata caagatgctc taggcaactg tattgatatc aatgagtgtt
1021 tgagtatcag tgccccgtgc cctatcgggc atacatgcat caacacagag ggctcctaca
1081 cgtgccagaa gaacgtgccc aactgtggcc gtggctacca tctcaacgag gagggaacgc
1141 gctgtgttga tgtggacgag tgcgcgccac ctgctgagcc ctgtgggaag ggacatcgct
1201 gcgtgaactc tcccggcagt ttccgctgcg aatgcaagac gggttactat tttgacggca
1261 tcagcaggat gtgtgtcgat gtcaacgagt gccagcgcta ccccgggcgc ctgtgtggcc
1321 acaagtgcga gaacacgctg ggctcctacc tctgcagctg ttccgtgggc ttccggctct
1381 ctgtggatgg caggtcatgt gaagacatca atgagtgcag cagcagcccc tgtagccagg
1441 agtgtgccaa cgtctacggc tcctaccagt gttactgccg gcgaggctac cagctcagcg
1501 atgtggatgg agtcacctgt gaagacatcg acgagtgcgc cctgcccacc gggggccaca
1561 tctgctccta ccgctgcatc aacatccctg gaagcttcca gtgcagctgc ccctcgtctg
1621 gctacaggct ggcccccaat ggccgcaact gccaagacat tgatgagtgt gtgactggca
1681 tccacaactg ctccatcaac gagacctgct tcaacatcca gggcggcttc cgctgcctgg
1741 ccttcgagtg ccctgagaac taccgccgct ccgcagccac ccgctgtgag cgcttgcctt
1801 gccatgagaa tcgggagtgc tccaagctgc ctctgagaat aacctactac cacctctctt
1861 tccccaccaa catccaagcg cccgcggtgg ttttccgcat gggcccctcc agtgctgtcc
1921 ccggggacag catgcagctg gccatcaccg gcggcaatga ggagggcttt ttcaccaccc
1981 ggaaggtgag cccccacagt ggggtggtgg ccctcaccaa gcctgtcccc gagcccaggg
2041 acttgctcct gaccgtcaag atggatctct ctcgccacgg caccgtcagc tcctttgtgg
2101 ccaagctttt catctttgtg tctgcagagc tctgagcact cgcttcgcgt cgcggggtct
2161 ccctcctgtt gctttcctaa ccctgccctc cggggcgtta ataaagtctt agcaagcgtc
2221 cc
//