LOCUS AK300801 1914 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ51317 complete cds, highly similar to
High-affinity choline transporter 1.
ACCESSION AK300801
VERSION AK300801.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1914)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1914
/cell_line="SK-N-SH"
/cell_type="neuroblastoma"
/clone="SKNSH2002342"
/clone_lib="SKNSH2"
/db_xref="H-InvDB:HIT000495425"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 378..1805
/codon_start=1
/note="highly similar to High-affinity choline
transporter 1"
/protein_id="BAG62459.1"
/transl_table=1
/translation="MRSKGYVTMLDPFQQIYGKRMGGLLFIPALMGVMFWAAAIFSAL
GATISVIIDVDMHISVIISALIATLYTLVGGLYSVAYTDVVQLFCIFVGLWISVPFAL
SHPAVADIGFTAVHAKYQKPWLGTVDSSEVYSWLDSFLLLMLGGIPWQAYFQRVLSSS
SATYAQVLSFLAAFGCLVMAIPAILIGAIGASTDWNQTAYGLPDPKTTEEADMILPIV
LQYLCPVYISFFGLGAVSAAVMSSADSSILSASSMFARNIYQLSFRQNASDKEIVWVM
RITVFVFGASATAMALLTKTVYGLWYLSSDLVYIVIFPQLLCVLFVKGTNTYGAVAGY
VSGLFLRITGGEPYLYLQPLIFYPGYYPDDNGIYNQKFPFKTLAMVTSFLTNICISYL
AKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVKNENIKLDELALVKPRQSMTLSS
TFTNKEAFLDVDSSPEGSGTEDNLQ"
BASE COUNT 442 a 494 c 434 g 544 t
ORIGIN
1 gattcccacc tcgccctgcg ccgcgcgccc tccgccggcg ccaacacctg tcaactctgc
61 gcgctcccag gttcttggag acgccgagtg aggagccgcc ctgaccgccc ggccccgctc
121 cgcactgcac cccgacccac cccgcaccct ccctttctgc accctcgcac ccacacccct
181 cgcggttcca gggggcggcg gccccgggcg gagcgctttc gcgtgcagcc accactccag
241 ctacctgggt cggaggaggg tatatcaatg gcacagctga agcagtttat gtaccaggtt
301 atggcctagc ttgggctcag gcaccaattg gatattctct tagtctgatt ttaggtggcc
361 tgttctttgc aaaacctatg cgttcaaagg ggtatgtgac catgttagac ccgtttcagc
421 aaatctatgg aaaacgcatg ggcggactcc tgtttattcc tgcactgatg ggagtaatgt
481 tctgggctgc agcaattttc tctgctttgg gagccaccat cagcgtgatc atcgatgtgg
541 atatgcacat ttctgtcatc atctctgcac tcattgccac tctgtacaca ctggtgggag
601 ggctctattc tgtggcctac actgatgtcg ttcagctctt ttgcattttt gtagggctgt
661 ggatcagcgt cccctttgca ttgtcacatc ctgcagtcgc agacatcggg ttcactgctg
721 tgcatgccaa ataccaaaag ccgtggctgg gaactgttga ctcatctgaa gtctactctt
781 ggcttgatag ttttctgttg ttgatgctgg gtggaatccc atggcaagca tactttcaga
841 gggttctctc ttcttcctca gccacctatg ctcaagtgct gtccttcctg gcagctttcg
901 ggtgcctggt gatggccatc ccagccatac tcattggggc cattggagca tcaacagact
961 ggaaccagac tgcatatggg cttccagatc ccaagactac agaagaggca gacatgattt
1021 taccaattgt tctgcagtat ctctgccctg tgtatatttc tttctttggt cttggtgcag
1081 tttctgctgc tgttatgtca tcagcagatt cttccatctt gtcagcaagt tccatgtttg
1141 cacggaacat ctaccagctt tccttcagac aaaatgcttc ggacaaagaa atcgtttggg
1201 ttatgcgaat cacagtgttt gtgtttggag catctgcaac agccatggcc ttgctgacga
1261 aaactgtgta tgggctctgg tacctcagtt ctgaccttgt ttacatcgtt atcttccccc
1321 agctgctttg tgtactcttt gttaagggaa ccaacaccta tggggccgtg gcaggttatg
1381 tttctggcct cttcctgaga ataactggag gggagccata tctgtatctt cagcccttga
1441 tcttctaccc tggctattac cctgatgata atggtatata taatcagaaa tttccattta
1501 aaacacttgc catggttaca tcattcttaa ccaacatttg catctcctat ctagccaagt
1561 atctatttga aagtggaacc ttgccaccta aattagatgt atttgatgct gttgttgcaa
1621 gacacagtga agaaaacatg gataagacaa ttcttgtcaa aaatgaaaat attaaattag
1681 atgaacttgc acttgtgaag ccacgacaga gcatgaccct cagctcaact ttcaccaata
1741 aagaggcctt ccttgatgtt gattccagtc cagaagggtc tgggactgaa gataatttac
1801 agtgacccca tctaaataaa atactgcttt tgcaaacaga acactgtaat agggtagttc
1861 tggagagatg gtatgcagca tacaaaaata tattaaaaat ataaacaatg ttca
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