LOCUS AK300800 2254 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54554 complete cds, highly similar to
Pyruvate kinase isozymes M1/M2 (EC 2.7.1.40).
ACCESSION AK300800
VERSION AK300800.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2254)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2254
/cell_line="SK-N-SH"
/cell_type="neuroblastoma"
/clone="SKNSH2001284"
/clone_lib="SKNSH2"
/db_xref="H-InvDB:HIT000495424"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 159..1709
/codon_start=1
/note="highly similar to Pyruvate kinase isozymes M1/M2
(EC 2.7.1.40)"
/protein_id="BAG62458.1"
/transl_table=1
/translation="MSPEAQPQRTKGPQQPCRSPIVKPGLPSFRPSSCTQPWLTHSWS
TCAAWTLIHHPSQPGTLASSVPLYHAETIKNVRTATESFASDPILYRPVAVALDTKGP
EIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIY
VDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGV
EQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIM
VARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVA
NAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSD
PTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHL
YRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTN
TMRVVPVP"
BASE COUNT 501 a 643 c 644 g 466 t
ORIGIN
1 gcacagcagc tgcacgcgcc gtggctccgg atctcttcgt ctttgcagcg tagcccgagt
61 cggtcagcgc cggagctctc cagggcacac cgtattcagc tctgagcggt ctttgctagt
121 gaggccaagg agccaccctg agccaaaagg ggagcattat gtcaccggaa gcccaacccc
181 agagaaccaa aggacctcag cagccatgtc gaagccccat agtgaagccg ggactgcctt
241 cattcagacc cagcagctgc acgcagccat ggctgacaca ttcctggagc acatgtgccg
301 cctggacatt gattcaccac ccatcacagc ccggaacact ggcatcatct gtaccattgt
361 accatgcgga gaccatcaag aatgtgcgca cagccacgga aagctttgct tctgacccca
421 tcctctaccg gcccgttgct gtggctctag acactaaagg acctgagatc cgaactgggc
481 tcatcaaggg cagcggcact gcagaggtgg agctgaagaa gggagccact ctcaaaatca
541 cgctggataa cgcctacatg gaaaagtgtg acgagaacat cctgtggctg gactacaaga
601 acatctgcaa ggtggtggaa gtgggcagca agatctacgt ggatgatggg cttatttctc
661 tccaggtgaa gcagaaaggt gccgacttcc tggtgacgga ggtggaaaat ggtggctcct
721 tgggcagcaa gaagggtgtg aaccttcctg gggctgctgt ggacttgcct gctgtgtcgg
781 agaaggacat ccaggatctg aagtttgggg tcgagcagga tgttgatatg gtgtttgcgt
841 cattcatccg caaggcatct gatgtccatg aagttaggaa ggtcctggga gagaagggaa
901 agaacatcaa gattatcagc aaaatcgaga atcatgaggg ggttcggagg tttgatgaaa
961 tcctggaggc cagtgatggg atcatggtgg ctcgtggtga tctaggcatt gagattcctg
1021 cagagaaggt cttccttgct cagaagatga tgattggacg gtgcaaccga gctgggaagc
1081 ctgtcatctg tgctactcag atgctggaga gcatgatcaa gaagccccgc cccactcggg
1141 ctgaaggcag tgatgtggcc aatgcagtcc tggatggagc cgactgcatc atgctgtctg
1201 gagaaacagc caaaggggac tatcctctgg aggctgtgcg catgcagcac ctgattgccc
1261 gtgaggcaga ggctgccatc taccacttgc aattatttga ggaactccgc cgcctggcgc
1321 ccattaccag cgaccccaca gaagccaccg ccgtgggtgc cgtggaggcc tccttcaagt
1381 gctgcagtgg ggccataatc gtcctcacca agtctggcag gtctgctcac caggtggcca
1441 gataccgccc acgtgccccc atcattgctg tgacccggaa tccccagaca gctcgtcagg
1501 cccacctgta ccgtggcatc ttccctgtgc tgtgcaagga cccagtccag gaggcctggg
1561 ctgaggacgt ggacctccgg gtgaactttg ccatgaatgt tggcaaggcc cgaggcttct
1621 tcaagaaggg agatgtggtc attgtgctga ccggatggcg ccctggctcc ggcttcacca
1681 acaccatgcg tgttgttcct gtgccgtgat ggaccccaga gcccctcctc cagcccctgt
1741 cccaccccct tcccccagcc catccattag gccagcaacg cttgtagaac tcactctggg
1801 ctgtaacgtg gcactggtag gttgggacac cagggaagaa gatcaacgcc tcactgaaac
1861 atggctgtgt ttgcagcctg ctctagtggg acagcccaga gcctggctgc ccatcatgtg
1921 gccccaccca atcaagggaa gaaggaggaa tgctggactg gaggcccctg gagccagatg
1981 gcaagagggt gacagcttcc tttcctgtgt gtactctgtc cagttccttt agaaaaaatg
2041 gatgcccaga ggactcccaa ccctgccttg gggtcaagaa acagccagca agagttaggg
2101 gccttagggc actgggctgt tgttccattg aagccgactc tggccctggc ccttacttgc
2161 ttctctagct ctctaggcct ctccagtttg cacctgtccc caccctccac tcagctgtcc
2221 tgcagcaaac actccaccct ccaccttcca tttt
//