LOCUS AK300787 2199 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57604 complete cds, highly similar to GMP
synthase (glutamine-hydrolyzing) (EC 6.3.5.2).
ACCESSION AK300787
VERSION AK300787.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2199)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2199
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2008218"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000495411"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 253..2181
/codon_start=1
/note="highly similar to GMP synthase
(glutamine-hydrolyzing) (EC 6.3.5.2)"
/protein_id="BAG62449.1"
/transl_table=1
/translation="MLEETLRMATTTIKEQGFRAIIISGGPNSVYAEDAPWFDPAIFT
IGKPVLGICYGMQMMNKVFGGTVHKKSVREDGVFNISVDNTCSLFRGLQKEEVVLLTH
GDSVDKVADGFKVVARSGNIVAGIANESKKLYGAQFHPEVGLTENGKVILKNFLYDIA
GCSGTFTVQNRELECIREIKERVGTSKVLVLLSGGVDSTVCTALLNRALNQEQVIAVH
IDNGFMRKRESQSVEEALKKLGIQVKVINAAHSFYNGTTTLPISDEDRTPRKRISKTL
NMTTSPEEKRKIIGDTFVKIANEVIGEMNLKPEEVFLAQGTLRPDLIESASLVASGKA
ELIKTHHNDTELIRKLREEGKVIEPLKDFHKDEVRILGRELGLPEELVSRHPFPGPGL
AIRVICAEEPYICKDFPETNNILKIVADFSASVKKPHTLLQRVKACTTEEDQEKLMQI
TSLHSLNAFLLPIKTVGVQGDCRSYSYVCGISSKDEPDWESLIFLARLIPRMCHNVNR
VVYIFGPPVKEPPTDVTPTFLTTGVLSTLRQADFEAHNILRESGYAGKISQMPVILTP
LHFDRDPLQKQPSCQRSVVIRTFITSDFMTGIPATPGNEIPVEVVLKMVTEIKKIPGI
SRIMYDLTSKPPGTTEWE"
BASE COUNT 633 a 471 c 513 g 582 t
ORIGIN
1 gtgcgcgctg ctggtcttct ctcccgcggc gctggggccc gcgctccgct gctgttgctc
61 cattcggcgc ttttctggcg gctggctcct ctccgctgcc ggctgctcct cgaccaggcc
121 tccttctcaa cctcagcccg cggcgccgac ccttccggca ccctcccgcc ccgtctcgta
181 ctgtcgccgt caccgccgcg gctccggccc tggccccgat ggctctgtgc aacggagact
241 ccaagctgga gaatgctgga ggagacctta aggatggcca ccaccactat aaaggaacaa
301 ggattccgtg ctattatcat ctctggagga cctaattctg tgtatgctga agatgctccc
361 tggtttgatc cagcaatatt cactattggc aagcctgttc ttggaatttg ctatggtatg
421 cagatgatga ataaggtatt tggaggtact gtgcacaaaa aaagtgtcag agaagatgga
481 gttttcaaca ttagtgtgga taatacatgt tcattattca ggggccttca gaaggaagaa
541 gttgttttgc ttacacatgg agatagtgta gacaaagtag ctgatggatt caaggttgtg
601 gcacgttctg gaaacatagt agcaggcata gcaaatgaat ctaaaaagtt atatggagca
661 cagttccacc ctgaagttgg ccttacagaa aatggaaaag taatactgaa gaatttcctt
721 tatgatatag ctggatgcag tggaaccttc accgtgcaga acagagaact tgagtgtatt
781 cgagagatca aagagagagt aggcacgtca aaagttttgg ttttactcag tggtggagta
841 gactcaacag tttgtacagc tttgctaaat cgtgctttga accaagaaca agtcattgct
901 gtgcacattg ataatggctt tatgagaaaa cgagaaagcc agtctgttga agaggccctc
961 aaaaagcttg gaattcaggt caaagtgata aatgctgctc attctttcta caatggaaca
1021 acaaccctac caatatcaga tgaagataga accccacgga aaagaattag caaaacgtta
1081 aatatgacca caagtcctga agagaaaaga aaaatcattg gggatacttt tgttaagatt
1141 gccaatgaag taattggaga aatgaacttg aaaccagagg aggttttcct tgcccaaggt
1201 actttacggc ctgatctaat tgaaagtgca tcccttgttg caagtggcaa agctgaactc
1261 atcaaaaccc atcacaatga cacagagctc atcagaaagt tgagagagga gggaaaagta
1321 atagaacctc tgaaagattt tcataaagat gaagtgagaa ttttgggcag agaacttgga
1381 cttccagaag agttagtttc caggcatcca tttccaggtc ctggcctggc aatcagagta
1441 atatgtgctg aagaacctta tatttgtaag gactttcctg aaaccaacaa tattttgaaa
1501 atagtagctg atttttctgc aagtgttaaa aagccacata ccctattaca gagagtcaaa
1561 gcctgcacaa cagaagagga tcaggagaag ctgatgcaaa ttaccagtct gcattcactg
1621 aatgccttct tgctgccaat taaaactgta ggtgtgcagg gtgactgtcg ttcctacagt
1681 tacgtgtgtg gaatctccag taaagatgaa cctgactggg aatcacttat ttttctggct
1741 aggcttatac ctcgcatgtg tcacaacgtt aacagagttg tttatatatt tggcccacca
1801 gttaaagaac ctcctacaga tgttactccc actttcttga caacaggggt gctcagtact
1861 ttacgccaag ctgattttga ggcccataac attctcaggg agtctgggta tgctgggaaa
1921 atcagccaga tgccggtgat tttgacacca ttacattttg atcgggaccc acttcaaaag
1981 cagccttcat gccagagatc tgtggttatt cgaaccttta ttactagtga cttcatgact
2041 ggtatacctg caacacctgg caatgagatc cctgtagagg tggtattaaa gatggtcact
2101 gagattaaga agattcctgg tatttctcga attatgtatg acttaacatc aaagccccca
2161 ggaactactg agtgggagta ataaacttct tgttctatt
//