LOCUS AK300755 1906 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ57439 complete cds, highly similar to
Kelch-like protein 20.
ACCESSION AK300755
VERSION AK300755.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1906)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1906
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2004354"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000495379"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 144..1406
/codon_start=1
/note="highly similar to Kelch-like protein 20"
/protein_id="BAG62422.1"
/transl_table=1
/translation="MVHGRKANAQVMESEEFMLLPANQLIDIISSDELNVRSEEQVFN
AVMAWVKYSIQERRPQLPQVLQHVRLPLLSPKFLVGTVGSDPLIKSDEECRDLVDEAK
NYLLLPQERPLMQGPRTRPRKPIRCGEVLFAVGGWCSGDAISSVERYDPQTNEWRMVA
SMSKRRCGVGVSVLDDLLYAVGGHDGSSYLNSVERYDPKTNQWSSDVAPTSTCRTSVG
VAVLGGFLYAVGGQDGVSCLNIVERYDPKENKWTRVASMSTRRLGVAVAVLGGFLYAV
GGSDGTSPLNTVERYNPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVGGRDDTTELSS
AERYNPRTNQWSPVVAMTSRRSGVGLAVVNGQLMAVGGFDGTTYLKTIEVFDPDANTW
RLYGGMNYRRLGGGVGVIKMTHCESHIW"
BASE COUNT 544 a 360 c 505 g 497 t
ORIGIN
1 ctacgagcgg catcctgcct gcgttagcgg cggtggagga ggaggcagag aggagtggag
61 ggcggagtag acggaggagg ctgctgcaga gaagaaagtg tcagagccgg ttcggcttta
121 gagtgtggtg aagggtactt ttcatggtgc atggaaggaa agccaatgcg caggtaatgg
181 agagtgaaga gttcatgttg cttccagcca atcaactcat tgatataata tccagtgatg
241 agctaaacgt tcgcagtgaa gaacaagtgt tcaatgcagt gatggcctgg gtcaaataca
301 gtattcagga aagacgtcct caattacccc aggtgctgca gcatgttcgt ttgcctttgc
361 ttagtcccaa gttcctggtc ggcacagtag gctctgatcc cctcatcaaa agtgatgaag
421 aatgcagaga cttggtagat gaggctaaaa actacctcct attgccgcaa gaacgaccac
481 taatgcaagg accaaggacg agaccacgga aacctatccg atgtggggaa gtactctttg
541 cagttggtgg ttggtgcagt ggagatgcca tttccagtgt tgaacgatat gatccacaga
601 ccaatgaatg gagaatggtg gcttcaatga gcaaaaggag atgcggagtt ggggtcagtg
661 ttcttgatga tctgttatat gcagtaggag gccatgatgg atcctcttat ctcaatagtg
721 ttgaaaggta tgaccccaaa acaaaccagt ggagcagtga tgtggcccct acaagcacct
781 gcaggacaag tgttggtgta gcagtacttg gaggctttct ttatgctgtg ggtggccagg
841 atggtgtgtc ttgcctcaac attgttgaga ggtatgatcc gaaggagaac aagtggactc
901 gggtagcttc tatgagtacc agaagactag gtgtggctgt ggctgtgtta ggagggttct
961 tatatgctgt aggtggctct gacgggacat ctcctctcaa cacagtggaa cgttacaatc
1021 ctcaggaaaa cagatggcac actatagccc ctatggggac ccggaggaaa cacctaggct
1081 gtgcagtata tcaggacatg atctatgctg taggaggtag agatgacact acagagctga
1141 gcagtgctga gagatacaac cccagaacca accagtggtc tccagtggtg gccatgacat
1201 cacgccgtag tggagttggc ctggcagtgg tcaatggaca gctcatggca gtaggaggtt
1261 ttgatggcac aacatacttg aagaccatag aagtttttga tcctgatgcc aatacatgga
1321 ggttatatgg cgggatgaat taccgtcggc tagggggtgg cgtaggagtt attaaaatga
1381 cacattgtga atcccatatt tggtgaacac agagaagaca gtcttgtata tattcctctg
1441 tattctgggg agctttgacc ttggagcttt gtacagcttg agaaaacatt agaacaaatt
1501 ttattatttg ccggtgcctc aacaaatgga aatacaatcc aatgaaagta cttcacctgc
1561 aagatgcaca ataattttca actctgtgca gaagaatatt tatttttggt tttaatttat
1621 catggttttt tgttgttttc gttttgaact tatccttcct cccacaaaaa aagaaaagaa
1681 gaaaaaattc caagcagcaa aacttacttt gtttgtaagg tattcattta ggtttgaaaa
1741 tactatttaa taagggcaga agggctatat atgatttggc tattatttct agacactcca
1801 tccacatgat tccactaaca aggattacca ggaataaagg taggtatgca aaatgtatta
1861 gctacccatt attctcgcac taaccaccag aagacttgaa aatctt
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