LOCUS AK300754 2720 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50873 complete cds, highly similar to DNA
replication licensing factor MCM3.
ACCESSION AK300754
VERSION AK300754.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2720)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2720
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2004284"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000495378"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 59..2347
/codon_start=1
/note="highly similar to DNA replication licensing factor
MCM3"
/protein_id="BAG62421.1"
/transl_table=1
/translation="MWSYGRLREITWTSWTTRLLNNAFEELVAFQRALKDFVASIDAT
YAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYC
PATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPE
KAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACN
VKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLL
GGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTA
AVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGI
HARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDRE
ISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHG
TKKKKEKMVGAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSP
VTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSED
ESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADS
QETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQ
AALSKMQDDNQVMVSEGIIFLI"
BASE COUNT 680 a 672 c 751 g 617 t
ORIGIN
1 gactttggtg gaggtagttc tttggcagcg ggcatggcgg gtaccgtggt gctggacgat
61 gtggagctac gggaggctca gagagattac ctggacttcc tggacgacga ggcttctgaa
121 caatgccttt gaggagctgg ttgccttcca gcgggcctta aaggattttg tggcctccat
181 tgatgctacc tatgccaagc agtatgagga gttctacgta ggactggaag gcagctttgg
241 ctccaagcac gtctccccgc ggactcttac ctcctgcttc ctcagctgtg tggtctgtgt
301 ggagggcatt gtcactaaat gttctctagt tcgtcccaaa gtcgtccgca gtgtccacta
361 ctgtcctgct actaagaaga ccatagagcg acgttattct gatctcacca ccctggtggc
421 ctttccctcc agctctgtct atcctaccaa ggatgaggag aacaatcccc ttgagacaga
481 atatggcctt tctgtctaca aggatcacca gaccatcacc atccaggaga tgccggagaa
541 ggccccagcc ggccagctcc cccgctctgt ggacgtcatt ctggatgatg acttggtgga
601 taaagcgaag cctggtgacc gggttcaggt ggtgggaacc taccgttgcc ttcctggaaa
661 gaagggaggc tacacctctg ggaccttcag gactgtcctg attgcctgta atgttaagca
721 gatgagcaag gatgctcagc cctctttctc tgctgaggat atagccaaga tcaagaagtt
781 cagtaaaacc cgatccaagg atatctttga ccagctggcc aagtcattgg ccccaagtat
841 ccatgggcat gactatgtca agaaagcaat cctctgcttg ctcttgggag gggtggaacg
901 agacctagaa aatggcagcc acatccgtgg ggacatcaat attcttctaa taggagaccc
961 atccgttgcc aagtctcagc ttctgcggta tgtgctttgc actgcacccc gagctatccc
1021 caccactggc cggggctcct ctggagtggg tctgacggct gctgtcacca cagaccagga
1081 aacaggagag cgccgtctgg aggcaggggc catggtcctg gctgaccgag gcgtggtttg
1141 cattgatgaa tttgacaaaa tgtctgacat ggatcgcaca gccatccatg aagtgatgga
1201 gcagggtcga gtgaccattg ccaaggctgg catccatgct cggctgaatg cccgctgcag
1261 tgttttggca gctgccaacc ctgtctacgg caggtatgac cagtataaga ctccaatgga
1321 gaacattggg ctacaggact cactgctgtc acgatttgac ttgctcttca tcatgctgga
1381 tcagatggat cctgagcagg atcgggagat ctcagaccat gtccttcgga tgcaccgtta
1441 cagagcacct ggggagcagg atggcgatgc tatgcccttg ggtagtgctg tggatatcct
1501 ggccacagat gatcccaact ttagccagga agatcagcag gacacccaga tttatgagaa
1561 gcatgacaac cttctacatg ggaccaagaa gaaaaaggag aagatggtgg gtgcagcatt
1621 catgaagaag tacatccatg tggccaaaat catcaagcct gtcctgacac aggagtcggc
1681 cacctacatt gcagaagagt attcacgcct gcgcagccag gatagcatga gctcagacac
1741 cgccaggaca tctccagtta cagcccgaac actggaaact ctgattcgac tggccacagc
1801 ccatgcgaag gcccgcatga gcaagactgt ggacctgcag gatgcagagg aagctgtgga
1861 gttggtccag tatgcttact ttaagaaggt tctggagaag gagaagaaac gtaagaagcg
1921 aagtgaggat gaatcagaga cagaagatga agaggagaaa agccaagagg accaggagca
1981 gaagaggaag agaaggaaga ctcgccagcc agatgccaaa gatggggatt catacgaccc
2041 ctatgacttc agtgacacag aggaggaaat gcctcaagta cacactccaa agacggcaga
2101 ctcacaggag accaaggaat cccagaaagt ggagttgagt gaatccaggt tgaaggcatt
2161 caaggtggcc ctcttggatg tgttccggga agctcatgcg cagtcaatcg gcatgaatcg
2221 cctcacagaa tccatcaacc gggacagcga agagcccttc tcttcagttg agatccaggc
2281 tgctctgagc aagatgcagg atgacaatca ggtcatggtg tctgagggca tcatcttcct
2341 catctgagga ggcctcgtct ctgaacttgg gttgtgccga gagagcttgt tctgtgtttc
2401 ccaccctctc cctgacccaa gtctttgcct ctactccctt aacagtgttg aattcaactg
2461 aaggcgagga atgttggtga tgaagccgag ttcaggactc ggtgcccctt tgggaatggg
2521 tcatgaaagc tgccatgggg tgaggaaaga ggagacagtg ggagaggaca atgactattg
2581 catcttcatt gcaaaagcac tggctcatcc gccctacttc ccatcccaca caaacccaat
2641 tgtaaataac atatgacttc tgagtacttt tgggggcaca actgttttct gtttgctgtt
2701 tttttgtttt gttttttttt
//