LOCUS AK300734 1588 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60165 complete cds, highly similar to
Translation initiation factor eIF-2B subunit delta.
ACCESSION AK300734
VERSION AK300734.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1588)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1588
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC1000111"
/clone_lib="SKNMC1"
/db_xref="H-InvDB:HIT000495358"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 239..1177
/codon_start=1
/note="highly similar to Translation initiation factor
eIF-2B subunit delta"
/protein_id="BAG62407.1"
/transl_table=1
/translation="MKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPE
TGSAVSAAQCQGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQAR
KGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSK
VSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQ
QVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKR
EEEVMSMRNKEGMHLWVKLRNMLKTPGKANVSLFLP"
BASE COUNT 402 a 409 c 454 g 323 t
ORIGIN
1 ctaggactga gggcgatggc tgctgtggcc gtggctgttc gcgagggtga gtgaagagcc
61 gggcctgcct actggtacgc ggagccgacg agctccggac agctagtgcc gggccttgag
121 cgcctttggg ccccgccgct ccgttccagc gccacgctgg gctccggtct acacagtctc
181 gagcgcagtc ccgcgccgcg caccggctgg ttgctgtgag gaggactcgg gatccgggat
241 gaaggcggag cttccccctg ggcctggggc agtggggagg gaaatgacca aagaagaaaa
301 gctgcagctt cggaaggaaa agaaacagca gaagaagaaa cggaaggaag aaaagggggc
361 agaaccagag actggctctg ctgtatctgc agcccaatgt caaggcccaa ccagagaact
421 gccagaatcg ggcattcagt tgggcactcc tcgggagaaa gttccagctg gtcggagtaa
481 ggccgaactt cgggctgagc gtcgagccaa gcaggaggcc gagcgggccc tgaaacaggc
541 aagaaaaggg gaacaaggag gaccacctcc taaggccagc cccagcacag ctggagaaac
601 cccctcagga gtgaagcgtc tccctgagta ccctcaggtt gatgacctac ttctgagaag
661 gcttgttaaa aaaccagagc gtcaacaggt tcctacacga aaggattatg gatccaaagt
721 cagtctcttc tctcacctac cccagtacag cagacaaaac tctctgaccc agtttatgag
781 catcccatcc tctgtgatcc acccagccat ggtgcgactc ggcctgcagt actcccaggg
841 cctggtcagt ggctccaatg cccggtgtat tgccctgctt cgtgccttgc agcaggtgat
901 tcaggattac acaacaccac ctaatgaaga actctccagg gatctagtga ataaactaaa
961 accctacatg agcttcctga ctcagtgccg tcccctgtca gcgagcatgc acaacgccat
1021 caagttcctt aacaaggaaa tcaccagtgt gggcagttcc aagcgggaag aggaggtgat
1081 gagtatgaga aataaggaag gcatgcacct gtgggtaaaa ttaagaaaca tgttaaaaac
1141 tcccgggaag gccaacgtaa gccttttttt gccctagtca agggttaagg aatggtttgg
1201 ccattctgac ttgtgccatc tctgagccct ctataggctt gtcatcatac acatattcaa
1261 gcacctggca tgatcatcag agggataaaa gtgtccattt cttgttcact aaggatttta
1321 ctccatttag gccaagtcag aacttcgagc agccattgat cggtatgtgc aagagaagat
1381 tgtgctagca gctcaggcaa tttcacgctt tgcttaccag aagatcagta atggagatgt
1441 gatcctggta tatggatgct catctctggt atcacgaatt cttcaggagg cttggacaga
1501 gggccggcgg tttcgggtgg tagtggtgga cagccggcca tggctggaag gaaggcacac
1561 actacgttct ctagtccatg ctggtgtc
//