LOCUS AK300724 1591 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56883 complete cds, highly similar to
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1.
ACCESSION AK300724
VERSION AK300724.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1591)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1591
/clone="SKMUS2008322"
/clone_lib="SKMUS2"
/db_xref="H-InvDB:HIT000495348"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="skeletal muscle"
CDS 102..1322
/codon_start=1
/note="highly similar to
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1"
/protein_id="BAG62397.1"
/transl_table=1
/translation="MEEKTSRIKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCA
LAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGII
AENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGT
RYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQY
AYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEE
IQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRC
LLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDIT
REPEEALDTVPAHY"
BASE COUNT 428 a 413 c 390 g 360 t
ORIGIN
1 gggaaccaac gaacacctcc aggcttcctc tttgatgcca cccactggac ctgccttggg
61 ggtctgtaaa tgcaagagaa ccgagtgttg gataattagc gatggaagaa aaaacctcta
121 gaataaaagt gtttaattta ggccagtatc gacgagaggc agtgagctac aagaactatg
181 aattctttct tccagacaac atggaagccc tgcaaatcag gaagcagtgc gccctggcag
241 ccctgaagga tgttcacaac tatctcagcc atgaggaagg tcatgttgcg gtttttgatg
301 ccaccaacac taccagagaa cgacggtcac tgatcctgca gtttgcaaaa gaacatggtt
361 acaaggtgtt tttcattgag tccatttgta atgaccctgg cataattgca gaaaacatca
421 ggcaagtgaa acttggcagc cctgattata tagactgtga ccgggaaaag gttctggaag
481 actttctaaa gagaattgag tgctatgagg tcaactacca acccttggat gaggaactgg
541 acagccacct gtcctacatc aagatcttcg acgtgggcac acgctacatg gtgaaccgag
601 tgcaggatca catccagagc cgcacagtct actacctcat gaatatccat gtcacacctc
661 gctccatcta cctttgccga catggcgaga gtgaactcaa catcagaggc cgcatcggag
721 gtgactctgg cctctcagtt cgcggcaagc agtatgccta tgccctggcc aacttcattc
781 agtcccaggg catcagctcc ctgaaggtgt ggaccagtca catgaagagg accatccaga
841 cagctgaggc cctgggtgtc ccctatgagc agtggaaggc cctgaatgag attgatgcgg
901 gtgtctgtga ggagatgacc tatgaagaaa tccaggaaca ttaccctgaa gaatttgcac
961 tgcgagacca agataaatat cgctaccgct atcccaaggg agagtcctat gaggatctgg
1021 ttcagcgtct ggagccagtg ataatggagc tagaacgaca ggagaatgta ctggtgatct
1081 gccaccaggc tgtcatgcgg tgcctcctgg cctatttcct ggataaaagt tcagatgagc
1141 ttccatatct caagtgccct ctgcacacag tgctcaaact cactcctgtg gcttatggct
1201 gcaaagtgga atccatctac ctgaatgtgg aggccgtgaa cacacaccgg gagaagcctg
1261 agaatgtgga catcacccgg gaacctgagg aagccctgga tactgtccca gcccactact
1321 gagccctttc caagaagtca aactgcctgt gtcctcatcg ccttccacct ttaggaaatg
1381 ctatctttgc tcttctccta ctctgccttg gcctcactga ggcaccccac ttccagtgaa
1441 gaagtcctcc gcaactccca aacaagcctc gcttgctggc cgcaaccaag gagctatcta
1501 gctctggagg aaactttctt tcttaattcc tattctctga cgaataaaga cttactgcct
1561 acaagaggag aggaggaggt catggcgagg c
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