LOCUS AK300632 1763 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61413 complete cds, highly similar to
Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1).
ACCESSION AK300632
VERSION AK300632.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1763)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1763
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2009517"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000495256"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 616..1338
/codon_start=1
/note="highly similar to Serine/threonine-protein kinase
PAK 4 (EC 2.7.11.1)"
/protein_id="BAG62322.1"
/transl_table=1
/translation="MDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVM
EFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGR
VKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDG
EPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKH
PFLAKAGPPASIVPLMRQNRTR"
BASE COUNT 347 a 573 c 559 g 284 t
ORIGIN
1 gttggggatt caacatggcg gcgggagtgt ccgcggtggt ggcggtgcaa gagagctgag
61 ggaggcgcga gggcgcggag ttccaggtcg agcagttagg ccgcgagcga ctgcggcgcc
121 gagccgatga gtaacccgaa gcccctagag gagtggtcac ctgcctgagg gcacttctgt
181 cccaccagca tcagaccagg ccgcaccgag tccccggcac catgtttggg aagaggaaga
241 agcgggtgga gatctccgcg ccgtccaact tcgagcaccg cgtgcacacg ggcttcgacc
301 agcacgagca gaagttcacg gggctgcccc gccagtggca gagcctgatc gaggagtcgg
361 ctcgccggcc caagcccctc gtcgaccccg cctgcatcac ctccatccag cccggggccc
421 cctggccccc gctcaccaca gcgggagcca cagcgagtat cccatgagca gttccgggct
481 gccctgcagc tggtggtgga cccaggcgac ccccgctcct acctggacaa cttcatcaag
541 attggcgagg gctccacggg catcgtgtgc atcgccaccg tgcgcagctc gggcaagctg
601 gtggccgtca agaagatgga cctgcgcaag cagcagaggc gcgagctgct cttcaacgag
661 gtggtaatca tgagggacta ccagcacgag aatgtggtgg agatgtacaa cagctacctg
721 gtgggggacg agctctgggt ggtcatggag ttcctggaag gaggcgccct caccgacatc
781 gtcacccaca ccaggatgaa cgaggagcag atcgcggccg tgtgccttgc agtgctgcag
841 gccctgtcgg tgctccacgc ccagggcgtc atccaccggg acatcaagag cgactcgatc
901 ctgctgaccc atgatggcag ggtgaagctg tcagactttg ggttctgcgc ccaggtgagc
961 aaggaagtgc cccgaaggaa gtcgctggtc ggcacgccct actggatggc cccagagctc
1021 atctcccgcc ttccctacgg gccagaggta gacatctggt cgctggggat aatggtgatt
1081 gagatggtgg acggagagcc cccctacttc aacgagccac ccctcaaagc catgaagatg
1141 attcgggaca acctgccacc ccgactgaag aacctgcaca aggtgtcgcc atccctgaag
1201 ggcttcctgg accgcctgct ggtgcgagac cctgcccagc gggccacggc agccgagctg
1261 ctgaagcacc cattcctggc caaggcaggg ccgcctgcca gcatcgtgcc cctcatgcgc
1321 cagaaccgca ccagatgagg cccagcgccc ttcccctcaa ccaaagagcc ccccgggtca
1381 cccccgcccc actgaggcca gtagggggcc aggcctccca ctcctcccag cccgggagat
1441 gctccgcgtg gcaccaccct ccttgctggg ggtagatgag accctactac tgaactccag
1501 ttttgatctc gtgactttta gaaaaacaca gggactcgtg ggagcaagcg aggctcccag
1561 gacccccacc ctctgggaca ggccctcccc catgttcttc tgtctccagg aagggcagcg
1621 gccctcccat cactggaagt ctgcagtggg ggtcgctggg ggtggagaga acactaagag
1681 gtgaacatgt atgagtgtgt gcacgcgtgt gagtgtgcat gtgtgtgtgt gtgcaaaggt
1741 ccagccaccc cgtcctccag ccc
//