LOCUS AK300624 2336 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50686 complete cds, highly similar to
Calpastatin.
ACCESSION AK300624
VERSION AK300624.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2336)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2336
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2007816"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000495248"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 150..2060
/codon_start=1
/note="highly similar to Calpastatin"
/protein_id="BAH13316.1"
/transl_table=1
/translation="MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSL
PKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKSGMDAALDDLIDTLGGPEETEEEN
TTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAI
DALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQA
LEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPAMDKD
GKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEA
VCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKE
EDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGP
QNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQ
RQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYKHLLDDNGQDKPVKPPTKKS
EDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSA
KTTEETSKPKDD"
BASE COUNT 811 a 502 c 553 g 470 t
ORIGIN
1 agagagctgg gctggagctc caagcggaaa ccgcgggagc cgagcccagc taggaatgca
61 gacctcctga aaaccaagcc gaggactgcg gggtccggtg tccacgcaga gtgtcagctt
121 cctctggtgc aaccagcaag tcttccagta tgaatcccac agaaaccaag gctgtaaaaa
181 cagaacctga gaagaagtca cagtcaacca agctgtctgt ggttcatgag aaaaaatccc
241 aagaaggaaa gccaaaagaa cacacagagc caaaaagcct acccaagcag gcatcagata
301 caggaagtaa cgatgctcac aataaaaaag cagtttccag atcagctgaa cagcagccat
361 cagagaaatc aacagaacca aagtcaggca tggatgctgc tttggatgac ttaatagata
421 ctttaggagg acctgaagaa actgaagaag aaaatacaac gtatactgga ccagaagttt
481 cagatccaat gagttccacc tacatagagg aattgggtaa aagagaagtc acaattcctc
541 caaaatatag ggaactattg gctaaaaagg aagggatcac agggcctcct gcagactctt
601 cgaaacccat agggccagat gatgctatag acgccttgtc atctgacttc acctgtgggt
661 cgcctacagc tgctggaaag aaaactgaaa aagaggaatc tacagaagtt ttaaaagctc
721 agtcagcagg gacagtcaga agtgctgctc caccccaaga gaagaaaaga aaggtggaga
781 aggatacaat gagtgatcaa gcactcgagg ctctgtcggc ttcactgggc acccggcaag
841 cagaacctga gctcgacctc cgctcaatta aggaagtcga tgaggcaaaa gctaaagaag
901 aaaaactaga gaagtgtggt gaggatgatg aaacaatccc atctgagtac agattaaaac
961 cagccatgga taaagatgga aaaccactat tgccagagcc tgaagaaaaa cccaagcctc
1021 ggagtgaatc agaactcatt gatgaacttt cagaagattt tgaccggtct gaatgtaaag
1081 agaaaccatc taagccaact gaaaagacag aagaatctaa ggccgctgct ccagctcctg
1141 tgtcggaggc tgtgtgtcgg acctccatgt gtagtataca gtcagcaccc cctgagccgg
1201 ctaccttgaa gggcacagtg ccagatgatg ctgtagaagc cttggctgat agcctgggga
1261 aaaaggaagc agatccagaa gatggaaaac ctgtgatgga taaagtcaag gagaaggcca
1321 aagaagaaga ccgtgaaaag cttggtgaaa aagaagaaac aattcctcct gattatagat
1381 tagaagaggt caaggataaa gatggaaagc cactcctgcc aaaagagtct aaggaacagc
1441 ttccacccat gagtgaagac ttccttctgg atgctttgtc tgaggacttc tctggtccac
1501 aaaatgcttc atctcttaaa tttgaagatg ctaaacttgc tgctgccatc tctgaagtgg
1561 tttcccaaac cccagcttca acgacccaag ctggagcccc accccgtgat acctcgcaga
1621 gtgacaaaga cctcgatgat gccttggata aactctctga cagtctagga caaaggcagc
1681 ctgacccaga tgagaacaaa ccaatggaag ataaagtaaa ggaaaaagct aaagctgaac
1741 atagagacaa gcttggagaa agagatgaca ctatcccacc tgaatacaaa catctcctgg
1801 atgataatgg acaggacaaa ccagtgaagc cacctacaaa gaaatcagag gattcaaaga
1861 aacctgcaga tgaccaagac cccattgatg ctctctcagg agatctggac agctgtccct
1921 ccactacaga aacctcacag aacacagcaa aggataagtg caagaaggct gcttccagct
1981 ccaaagcacc taagaatgga ggtaaagcga aggattcagc aaagacaaca gaggaaactt
2041 ccaagccaaa agatgactaa agaaatacaa gttaaggtat ctggtatctg catgtaaaat
2101 cttcagctgg tggatggtga cttttgaaga acaaaaggct ttggcaacag aaaacaattg
2161 ttctgggtga tttctagaat ggtttttgtt gagtctctga acatcctaaa tattggtttg
2221 ttattctttt ccagaaagaa aatgaatttg actggttcac ctgtgtactg agtattgata
2281 aactttgaat ttttttaatt gccttcaatt gggagagaaa gctttatatt tgtaag
//