LOCUS AK300620 2513 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59760 complete cds, highly similar to
1,4-alpha-glucan branching enzyme (EC 2.4.1.18).
ACCESSION AK300620
VERSION AK300620.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2513)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2513
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2007164"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000495244"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 54..2039
/codon_start=1
/note="highly similar to 1,4-alpha-glucan branching
enzyme (EC 2.4.1.18)"
/protein_id="BAG62313.1"
/transl_table=1
/translation="MNLAKVRYKQFSQILKNIGENEGGIDKFSRGYESFGVHRCADGG
LYCKEWAPGAEGVFLTGDFNGWNPFSYPYKKLDYGKWELYIPPKQNKSVLVPHGSKLK
VVITSKSGEILYRISPWAKYVVREGDNVNYDWIHWDPEHSYEFKHSGPKKPRSLRIYE
SHVGISSHEGKVASYKHFTCNVLPRIKGLGYNCIQLMAIMEHAYYASFGYQITSFFAA
SSRYGTPEELQELVDTAHSMGIIVLLDVVHSHASKNSADGLNMFDGTDSCYFHSGPRG
THDLWDSRLFAYSSWEVLRFLLSNIRWWLEEYRFDGFRFDGVTSMLYHHHGVGQGFSG
DYSEYFGLQVDEDALTYLMLANHLVHTLCPDSITIAEDVSGMPALCSPISQGGGGFDY
RLAMAIPDKWIQLLKEFKDEDWNMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSLA
FWLMDAEMYTNMSVLTPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWL
DFPRKGNNESYHYARRQFHLTDDDLLRYKFLNNFDRDMNRLEERYGWLAAPQAYVSEK
HEGNKIIAFERAGLLFIFNFHPSKSYTDYRVGTALPGKFKIVLDSDAAEYGGHQRLDH
STDFFSEAFEHNGRPYSLLVYIPSRVALILQNVDLPN"
BASE COUNT 724 a 445 c 556 g 788 t
ORIGIN
1 aatttcactt ggattcggcc cagattaaca gctgtttcta acttcgttgt cccatgaatc
61 ttgctaaagt gaggtataag cagtttagcc aaattttgaa gaacattgga gaaaatgaag
121 gtggtattga taagttttcc agaggctatg aatcatttgg cgtccacaga tgtgctgatg
181 gtggtttata ctgcaaagaa tgggccccgg gagcagaagg agtttttctt actggagatt
241 ttaatggttg gaatccattt tcgtacccat acaaaaaact ggattatgga aaatgggagc
301 tgtatatccc accaaagcag aataaatctg tactcgtgcc tcatggatcc aaattaaagg
361 tagttattac tagtaaaagc ggagagatct tgtatcgtat ttcaccgtgg gcaaagtatg
421 tggttcgtga aggtgataat gtgaattatg attggataca ctgggatcca gaacactcat
481 atgagtttaa gcattccgga ccaaagaagc cacggagtct aagaatttat gaatctcatg
541 tgggaatttc ttcccatgaa ggaaaagtag cttcttataa acattttaca tgcaatgtac
601 taccaagaat caaaggcctt ggatacaact gcattcagtt gatggcaatc atggagcatg
661 cttactatgc cagctttggt taccaaatca caagcttctt tgcagcttcc agccgttatg
721 gaacacctga agagctacaa gaactggtag acacagctca ttccatgggt atcatagtcc
781 tcttagatgt ggtacacagc catgcttcaa aaaattcagc agatggattg aatatgtttg
841 atgggacaga ttcctgttat tttcattctg gacctagagg gactcatgat ctttgggata
901 gcagattgtt tgcctactcc agctgggaag ttttaagatt ccttctgtca aacataagat
961 ggtggttgga agaatatcgc tttgatggat tccgttttga tggtgttacg tccatgcttt
1021 atcatcacca tggagtgggt caaggtttct caggtgatta cagtgaatat ttcggactac
1081 aagtagatga agatgccttg acttacctca tgttggcaaa tcatttggtt cacacgctgt
1141 gtcccgattc tataacaata gctgaggatg tatcaggaat gccagctctg tgctctccaa
1201 tttcccaggg agggggtggt tttgactatc gactagccat ggcaattcca gataagtgga
1261 ttcagctact taaagagttt aaagatgaag actggaacat gggcgatata gtatacacgc
1321 tcacaaacag gcgctacctt gaaaagtgca ttgcttatgc agagagccat gatcaggcat
1381 tggttgggga taagtcgctg gcattttggt tgatggatgc cgaaatgtat acaaacatga
1441 gtgtcctgac tccttttact ccagttattg atcgtggaat acagcttcat aaaatgattc
1501 gactcattac gcatgggctt ggtggagaag gctatctcaa tttcatgggt aatgaatttg
1561 ggcatcctga atggttagac ttcccaagaa aaggaaataa tgagagttac cattatgcca
1621 ggcggcagtt tcatttaact gacgacgacc ttcttcgcta caagttccta aataattttg
1681 acagggatat gaatagattg gaagaaagat atggttggct tgcagctcca caggcctacg
1741 tgagtgaaaa acatgaaggc aataagatca ttgcttttga aagagcaggt cttcttttca
1801 ttttcaactt ccatccaagc aagagctaca ctgactaccg agttggaaca gcattgccag
1861 ggaaattcaa aattgtgcta gattcagatg cagcggaata tggagggcat cagagactgg
1921 accacagcac tgactttttt tctgaggctt ttgaacataa tgggcgtccc tattctcttt
1981 tggtgtacat tccaagcaga gtggccctca tccttcagaa tgtggatctg ccgaattgaa
2041 gaggcctgat ttcagctcca ccagatgcag atttgtgttt tgttttcttg ttatcactgt
2101 cacacagctt ataacatgta tgcttttcag aatacagttg tctagccaag ccatcaagtg
2161 tctgaaattc aatattggtt tatgcaaata cagcaaactt ttatttaagt agatgggaga
2221 atatgtttaa aatattagga atcctagacc atattttcaa gtcatcttag cagctaggat
2281 tctcaaatgg aagtgttata tataatatgt taaaaacatt ttgctttcct ggctaattat
2341 ttgatccttt taaattcaaa tttgaatcat ttgtcatgta tgattatttc tgttaaatgt
2401 acacagtatt taagatggat atttggtggc tctatttgtt ctgatatctt ttggtctaaa
2461 ttatgaggta ccaagattgt ttctttgttt ctttttttca aattgtgttt aga
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