LOCUS AK300610 2337 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53659 complete cds, highly similar to Myosin
light chain kinase, smooth muscle (EC2.7.11.18).
ACCESSION AK300610
VERSION AK300610.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2337)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2337
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2005277"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000495234"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 133..2277
/codon_start=1
/note="highly similar to Myosin light chain kinase,
smooth muscle (EC2.7.11.18)"
/protein_id="BAG62305.1"
/transl_table=1
/translation="MKSRRPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFP
EDQKVRAGESVELFGKVTGTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQ
EHCGCYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGG
SAVQSYSIEIWDSANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQE
SELTTVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFG
QVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANI
VMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIM
CVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVI
CYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRL
DCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISG
LSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGS
AARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTC
KAVNSLGEATCTAELIVETMEEGEGEGEEEEE"
BASE COUNT 674 a 562 c 656 g 445 t
ORIGIN
1 aacaaacaag tgtccgagct ggcaggacga cccagcaggg tcagccgtga ggaggaggga
61 gcagagcgag gtccgtgtgg gagacttcct agaggagatg ctccagccag tgagaacacc
121 aaggccccag agatgaaatc ccggaggccc aagagctctc ttcctcccgt gctaggaact
181 gagagtgatg cgactgtgaa aaagaaacct gcccccaaga cacctccgaa ggcagcaatg
241 ccccctcaga tcatccagtt ccctgaggac cagaaggtac gcgcaggaga gtcagtggag
301 ctgtttggca aagtgacagg cactcagccc atcacctgta cctggatgaa gttccgaaag
361 cagatccagg aaagcgagca catgaaggtg gagaacagcg agaatggcag caagctcacc
421 atcctggccg cgcgccagga gcactgcggc tgctacacac tgctggtgga gaacaagctg
481 ggcagcaggc aggcccaggt caacctcact gtcgtggata agccagaccc cccagctggc
541 acaccttgtg cctctgacat tcggagctcc tcactgaccc tgtcctggta tggctcctca
601 tatgatgggg gcagtgctgt acagtcctac agcatcgaga tctgggactc agccaacaag
661 acgtggaagg aactagccac atgccgcagc acctctttca acgtccagga cctgctgcct
721 gaccacgaat ataagttccg tgtacgtgca atcaacgtgt atggaaccag tgagccaagc
781 caggagtctg aactcacaac ggtaggagag aaacctgaag agccgaagga tgaagtggag
841 gtgtcagatg atgatgagaa ggagcccgag gttgattacc ggacagtgac aatcaatact
901 gaacaaaaag tatctgactt ctacgacatt gaggagagat taggatctgg gaaatttgga
961 caggtctttc gacttgtaga aaagaaaact cgaaaagtct gggcagggaa gttcttcaag
1021 gcatattcag caaaagagaa agagaatatc cggcaggaga ttagcatcat gaactgcctc
1081 caccacccta agctggtcca gtgtgtggat gcctttgaag aaaaggccaa catcgtcatg
1141 gtcctggaga tcgtgtcagg aggggagctg tttgagcgca tcattgacga ggactttgag
1201 ctgacggagc gtgagtgcat caagtacatg cggcagatct cggagggagt ggagtacatc
1261 cacaagcagg gcatcgtgca cctggacctc aagccggaga acatcatgtg tgtcaacaag
1321 acgggcacca ggatcaagct catcgacttt ggtctggcca ggaggctgga gaatgcgggg
1381 tctctgaagg tcctctttgg caccccagaa tttgtggctc ctgaagtgat caactatgag
1441 cccatcggct acgccacaga catgtggagc atcggggtca tctgctacat cctagtcagt
1501 ggcctttccc ccttcatggg agacaacgat aacgaaacct tggccaacgt tacctcagcc
1561 acctgggact tcgacgacga ggcattcgat gagatctccg acgatgccaa ggatttcatc
1621 agcaatctgc tgaagaaaga tatgaaaaac cgcctggact gcacgcagtg ccttcagcat
1681 ccatggctaa tgaaagatac caagaacatg gaggccaaga aactctccaa ggaccggatg
1741 aagaagtaca tggcaagaag gaaatggcag aaaacgggca atgctgtgag agccattgga
1801 agactgtcct ctatggcaat gatctcaggg ctcagtggca ggaaatcctc aacagggtca
1861 ccaaccagcc cgctcaatgc agaaaaacta gaatctgaag aagatgtgtc ccaagctttc
1921 cttgaggctg ttgctgagga aaagcctcat gtaaaaccct atttctctaa gaccattcgc
1981 gatttagaag ttgtggaggg aagtgctgct agatttgact gcaagattga aggataccca
2041 gaccccgagg ttgtctggtt caaagatgac cagtcaatca gggagtcccg ccacttccag
2101 atagactacg atgaggacgg gaactgctct ttaattatta gtgatgtttg cggggatgac
2161 gatgccaagt acacctgcaa ggctgtcaac agtcttggag aagccacctg cacagcagag
2221 ctcattgtgg aaacgatgga ggaaggtgaa ggggaagggg aagaggaaga agagtgaaac
2281 aaagccagag aaaagcagtt tctaagtcat attaaaagga ctatttctct aaaactc
//