LOCUS AK300604 1440 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50684 complete cds, highly similar to Pendrin.
ACCESSION AK300604
VERSION AK300604.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1440)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1440
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2003950"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000495228"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 159..1262
/codon_start=1
/note="highly similar to Pendrin"
/protein_id="BAH13312.1"
/transl_table=1
/translation="MRHGLAVPVAGIISAAIVMIAILALGKLLEPLQKSVLAAVVIAN
LKGMFMQLCDIPRLWRQNKIDAVIWVFTCIVSIILGLDLGLLAGLIFGLLTVVLRVQF
PSWNGLGSIPSTDIYKSTKNYKNIEEPQGVKILRFSSPIFYGNVDGFKKCIKSTVGFD
AIRVYNKRLKALRKIQKLIKSGQLRATKNGIISDAVSTNNAFEPDEDIEDLEELDIPT
KEIEIQVDWNSELPVKVNVPKVPIHSLVLDCGAISFLDVVGVRSLRVIVKEFQRIDVN
VYFASLQDYVIEKLEQCGFFDDNIRKDTFFLTVHDAILYLQNQVKSQEGQGSILETIT
LIQDCKDTLELIETELTEEELDVQDEAMRTLAS"
BASE COUNT 418 a 280 c 331 g 411 t
ORIGIN
1 gtgcacattt caggctgcag ctgtcagcag caggaacttc tgctgctttc atttgagacg
61 gagcactgac aagctctcca gagcacatgg tcttcagcgg gtgcaggcaa atctcaaact
121 actgatgtcc attctcctga tgttacctcc aggctcaaat gaggcatggg ctggctgtcc
181 cagttgctgg catcatctct gctgcgattg tgatgatcgc cattcttgcc ctggggaagc
241 ttctggaacc cttgcagaag tcggtcttgg cagctgttgt aattgccaac ctgaaaggga
301 tgtttatgca gctgtgtgac attcctcgtc tgtggagaca gaataagatt gatgctgtta
361 tctgggtgtt tacgtgtata gtgtccatca ttctggggct ggatctcggt ttactagctg
421 gccttatatt tggactgttg actgtggtcc tgagagttca gtttccttct tggaatggcc
481 ttggaagcat ccctagcaca gatatctaca aaagtaccaa gaattacaaa aacattgaag
541 aacctcaagg agtgaagatt cttagatttt ccagtcctat tttctatggc aatgtcgatg
601 gttttaaaaa atgtatcaag tccacagttg gatttgatgc cattagagta tataataaga
661 ggctgaaagc gctgaggaaa atacagaaac taataaaaag tggacaatta agagcaacaa
721 agaatggcat cataagtgat gctgtttcaa caaataatgc ttttgagcct gatgaggata
781 ttgaagatct ggaggaactt gatatcccaa ccaaggaaat agagattcaa gtggattgga
841 actctgagct tccagtcaaa gtgaacgttc ccaaagtgcc aatccatagc cttgtgcttg
901 actgtggagc tatatctttc ctggacgttg ttggagtgag atcactgcgg gtgattgtca
961 aagaattcca aagaattgat gtgaatgtgt attttgcatc acttcaagat tatgtgatag
1021 aaaagctgga gcaatgcggg ttctttgacg acaacattag aaaggacaca ttctttttga
1081 cggtccatga tgctatactc tatctacaga accaagtgaa atctcaagag ggtcaaggtt
1141 ccattttaga aacgatcact ctcattcagg attgtaaaga tacccttgaa ttaatagaaa
1201 cagagctgac ggaagaagaa cttgatgtcc aggatgaggc tatgcgtaca cttgcatcct
1261 gaaagtgggt tcgggaggtc tctatgagca aggaatacaa gacaaaactt cctcaatgca
1321 ttgactattt cttcagactc aaaacactca ttcttttttc tattaagcca ttgaaagaga
1381 agcactaaga ctgcttctag gctttattta taaaataaac accttatccc taacatgggc
//