LOCUS AK300585 2545 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53201 complete cds, highly similar to
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
precursor (EC 1.6.5.3).
ACCESSION AK300585
VERSION AK300585.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2545)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2545
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2001366"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000495209"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 167..2179
/codon_start=1
/note="highly similar to NADH-ubiquinone oxidoreductase
75 kDa subunit, mitochondrial precursor (EC 1.6.5.3)"
/protein_id="BAG62283.1"
/transl_table=1
/translation="MQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWN
ILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRA
VEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSE
LSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILP
RMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSF
QGKDVAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTI
AGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSP
KILQDIASGSHPFSQVLKEAKKPMVVLGSSALQRNDGAAILAAVSSIAQKIRMTSGVT
GDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCF
IIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKII
RALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLA
DPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC"
BASE COUNT 753 a 479 c 617 g 696 t
ORIGIN
1 attgaataag cgacccggcc tcctaggggg tcgtcgtggt ccagacagtt tagcagaaca
61 gcctccgcgg ctccggggag aagcaatatg ttaaggatac ctgtaagaaa ggccttagta
121 ggcctttcta agtctcctaa aggatgtggc ttgtgagaag gttggcatgc agatccctcg
181 attctgttat catgaaaggt tgtctgttgc tggaaactgc aggatgtgcc ttgttgaaat
241 tgagaaagcc cctaaggttg tagctgcttg tgccatgcca gtaatgaagg gttggaatat
301 cctaacaaac tcagaaaaat ccaaaaaagc cagggaaggt gtgatggagt tcttattagc
361 aaatcaccca ttggactgtc ctatttgtga ccagggaggt gaatgtgacc tgcaggacca
421 gtccatgatg tttggaaatg ataggagccg atttttagag gggaagcgtg ctgtggaaga
481 caagaacatt gggccattgg taaagaccat catgacaaga tgtatacagt gtactcgctg
541 catcaggttt gcaagtgaga ttgcaggagt agatgatttg ggaacaacag gcagaggaaa
601 tgatatgcaa gttggcacat acattgaaaa gatgttcatg tctgaactgt ctgggaatat
661 cattgatatc tgccctgtag gtgccctaac ctctaagccc tatgccttta ctgcccggcc
721 ttgggaaaca agaaagacag aatccattga tgtaatggat gcggttggaa gtaatattgt
781 ggttagcaca agaactggag aagtgatgag gattttgcca cgtatgcatg aggacatcaa
841 tgaagagtgg atctctgata aaaccagatt tgcctatgat gggctaaaac gtcaaagact
901 taccgagcca atggtcagaa atgaaaaagg gcttttaacc tatacttctt gggaggatgc
961 tctctctcgc gtagctggaa tgttgcagag ttttcaaggc aaagatgtgg cagcaattgc
1021 aggtggcttg gtggatgctg aagccctggt agctctcaaa gatttgctta atagagtgga
1081 ctctgacacc ttatgcactg aagaggtctt ccccactgca ggagctggca cagatttgcg
1141 ttccaattat cttcttaata ctacaattgc tggtgtggaa gaggcagatg ttgttcttct
1201 ggttggtaca aacccacgtt ttgaggcacc actgtttaat gctagaattc gaaagagctg
1261 gctgcataat gacttaaaag tggcccttat aggcagtcca gtggacctca cttacacata
1321 tgaccacctg ggagactccc ccaaaattct tcaagacatt gcttcgggaa gccatccatt
1381 tagccaggtc ctaaaggaag ctaaaaaacc aatggtggtt ttaggcagtt ctgcactcca
1441 aagaaatgat ggagcagcaa ttcttgcagc tgtttctagc attgcacaaa agattcggat
1501 gactagtggt gttactggtg attggaaagt tatgaatatc cttcatagga ttgcaagtca
1561 agtagctgct ttggaccttg gctataagcc tggggtggaa gcaattcgga agaaccctcc
1621 caaggtgctg tttctcctgg gagcagatgg aggttgtatc acacgacagg atttgccaaa
1681 ggattgtttc attatttatc aaggacatca tggtgatgtt ggggctccca tagctgatgt
1741 tattctccca ggagctgctt acacagagaa gtctgctaca tatgtcaaca ctgagggtag
1801 agctcagcag actaaggtag cagtgacacc tcctggcttg gcaagagaag actggaaaat
1861 tataagagca ctctctgaga ttgctggaat gactcttcca tatgatactc tggatcaagt
1921 aaggaacaga ttggaagaag tctctcctaa tcttgttcga tatgatgata ttgaaggggc
1981 taattacttc cagcaagcaa atgagctctc aaagctagtg aaccagcagc ttcttgctga
2041 cccacttgtt ccacctcagc taactataaa agacttctac atgacagatt caattagcag
2101 agcctcacag acaatggcca aatgtgtcaa agctgtcaca gagggtgccc aggcagtaga
2161 ggaaccatcc atatgctgaa gcttctacta ggatcccagt tttgccgcag ataattaatg
2221 gacaactgta gtgcagtgat cctttacagg tttatttctt tgtaaaaaaa aataataata
2281 atttgaatca tgtaatattt aaggttatac tatgcctatt tgaaaatgat attagttatc
2341 aactttgcag tttgaaaaac atgtattgtg tgtaaaggtt aaataacaaa actatgcaga
2401 tgctcttaaa agcattgata acctttgtga cgaacataaa gagatcctta aattatgagt
2461 tgttggctta tcttcataaa taatttgtct gtaaaatgga tgaaatgaaa agagtttcaa
2521 ttaaaaccta cttttttcta gtgct
//