LOCUS AK300444 3307 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ53199 complete cds, highly similar to E3
ubiquitin-protein ligase NEDD4-like protein (EC 6.3.2.-).
ACCESSION AK300444
VERSION AK300444.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3307)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3307
/clone="PLACE7016473"
/clone_lib="PLACE7"
/db_xref="H-InvDB:HIT000495068"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 429..2993
/codon_start=1
/note="highly similar to E3 ubiquitin-protein ligase
NEDD4-like protein (EC 6.3.2.-)"
/protein_id="BAH13286.1"
/transl_table=1
/translation="MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSD
QRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHR
PSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVN
IAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLR
SCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDA
KGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLS
APLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRP
FFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNS
KITQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFE
ESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSA
TDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQIT
LNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNEN
KREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVN
DWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGS
NGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD"
BASE COUNT 938 a 804 c 785 g 780 t
ORIGIN
1 agttgtgtta aaacttgctg gtattccccc ggtatcagca gaggtgtgta cgggcactgc
61 tttaaaactg ggaaggagga agacgaggcc agggagccgg agggtcacca aggtagattt
121 ccagcagcgc tagtccagct gaacactttc cagccttgtt tttcagcagc tttgaggaaa
181 agtatagtga tccgtatgtg aaactttcat tgtacgtagc ggatgagaat agagaacttg
241 ctttggtcca gacaaaaaca attaaaaaga cactgaaccc aaaatggaat gaagaatttt
301 atttcagggt aaacccatct aatcacagac tcctatttga agtatttgac gaaaatagac
361 tgacacgaga cgacttcctg ggccaggtgg acgtgcccct tagtcacctt ccgacagaag
421 atccaaccat ggagcgaccc tatacattta aggactttct cctcagacca agaagtcata
481 agtctcgagt taagggattt ttgcgattga aaatggccta tatgccgaaa aatggaggtc
541 aagatgaaga aaacagtgac cagagggatg acatggagca tggatgggaa gttgttgact
601 caaatgactc ggcttctcag caccaagagg aacttcctcc tcctcctctg cctcccgggt
661 gggaagaaaa agtggacaat ttaggccgaa cttactatgt caaccacaac aaccggacca
721 ctcagtggca cagaccaagc ctgatggacg tgtcctcgga gtcggacaat aacatcagac
781 agatcaacca ggaggcagca caccggcgct tccgctcccg caggcacatc agcgaagact
841 tggagcccga gccctcggag ggcggggatg tccccgagcc ttgggagacc atttcagagg
901 aagtgaatat cgctggagac tctctcggtc tggctctgcc cccaccaccg gcctccccag
961 gatctcggac cagccctcag gagctgtcag aggaactaag cagaaggctt cagatcactc
1021 cagactccaa tggggaacag ttcagctctt tgattcaaag agaaccctcc tcaaggttga
1081 ggtcatgcag tgtcaccgac gcagttgcag aacagggcca tctaccaccg cccagtgccc
1141 cagctgggag agcgcgttca tcaactgtca cgggtggtga ggaaccaacg ccatcagtgg
1201 cctatgtaca taccacgccg ggtctgcctt caggctggga agaaagaaaa gatgctaagg
1261 ggcgcacata ctatgtcaat cataacaatc gaaccacaac ttggactcga cctatcatgc
1321 agcttgcaga agatggtgcg tccggatcag ccacaaacag taacaaccat ctaatcgagc
1381 ctcagatccg ccggcctcgt agcctcagct cgccaacagt aactttatct gccccgctgg
1441 agggtgccaa ggactcaccc gtacgtcggg ctgtgaaaga caccctttcc aacccacagt
1501 ccccacagcc atcaccttac aactccccca aaccacaaca caaagtcaca cagagcttct
1561 tgccacccgg ctgggaaatg aggatagcgc caaacggccg gcccttcttc attgatcata
1621 acacaaagac tacaacctgg gaagatccac gtttgaaatt tccagtacat atgcggtcaa
1681 agacatcttt aaaccccaat gaccttggcc cccttcctcc tggctgggaa gaaagaattc
1741 acttggatgg ccgaacgttt tatattgatc ataatagcaa aattactcag tgggaagacc
1801 caagactgca gagcccagct attactggtc cggctgtccc ttactccaga gaatttaagc
1861 agaaatatga ctacttcagg aagaaattaa agaaacctgc tgatatcccc aataggtttg
1921 aaatgaaact tcacagaaat aacatatttg aagagtccta tcggagaatt atgtccgtga
1981 aaagaccaga tgtcctaaaa gctagactgt ggattgagtt tgaatcagag aaaggtcttg
2041 actatggggg tgtggccaga gaatggttct tcttactgtc caaagagatg ttcaacccct
2101 actacggcct ctttgagtac tctgccacgg acaactacac ccttcagatc aaccctaatt
2161 caggcctctg taatgaggat catttgtcct acttcacttt tattggaaga gttgctggtc
2221 tggccgtatt tcatgggaag ctcttagatg gtttcttcat tagaccattt tacaagatga
2281 tgttgggaaa gcagataacc ctgaatgaca tggaatctgt ggatagtgaa tattacaact
2341 ctttgaaatg gatcctggag aatgacccta ctgagctgga cctcatgttc tgcatagacg
2401 aagaaaactt tggacagaca tatcaagtgg atttgaagcc caatgggtca gaaataatgg
2461 tcacaaatga aaacaaaagg gaatatatcg acttagtcat ccagtggaga tttgtgaaca
2521 gggtccagaa gcagatgaac gccttcttgg agggattcac agaactactt cctattgatt
2581 tgattaaaat ttttgatgaa aatgagctgg agttgctcat gtgcggcctc ggtgatgtgg
2641 atgtgaatga ctggagacag cattctattt acaagaacgg ctactgccca aaccaccccg
2701 tcattcagtg gttctggaag gctgtgctac tcatggacgc cgaaaagcgt atccggttac
2761 tgcagtttgt cacagggaca tcgcgagtac ctatgaatgg atttgccgaa ctttatggtt
2821 ccaatggtcc tcagctgttt acaatagagc aatggggcag tcctgagaaa ctgcccagag
2881 ctcacacatg ctttaatcgc cttgacttac ctccatatga aacctttgaa gatttacgag
2941 agaaacttct catggccgtg gaaaatgctc aaggatttga aggggtggat taagcaccct
3001 gtgcctcggg ggtggttgtt cttcaagcaa gttctgcttg cacttttgca tttgcctaac
3061 agacttttgc agaggcgatg gcagagagca gctgcaggca tggtccctgg agccgagcct
3121 tcaccacgca ctcgtccaag ttcggatgcg ggaacctggt cccagcttga gttcctgcct
3181 ttcccaccac aaattatcaa ctggttgatg tgtacactaa ttacatttta ggaggactta
3241 atgctattta tgttgtgcct ctgcaggcaa agcccttaat aaatatttta catcctttct
3301 aaaaaag
//