LOCUS AK300264 3232 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53192 complete cds, highly similar to Homo
sapiens Rho guanine nucleotide exchange factor (GEF) 10-like,
transcript variant 1, mRNA.
ACCESSION AK300264
VERSION AK300264.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3232)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3232
/clone="PLACE7001073"
/clone_lib="PLACE7"
/db_xref="H-InvDB:HIT000494888"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 5..3133
/codon_start=1
/note="highly similar to Homo sapiens Rho guanine
nucleotide exchange factor (GEF) 10-like, transcript
variant 1, mRNA"
/protein_id="BAG62025.1"
/transl_table=1
/translation="MLPSSSWGKRKLRGRLGWVRSDARGVMWARQEGLHHPHPHAVIR
CPSSSSSSVSCSGDSEEEDMGLLEVSVSDIKPPAPELGPMPEGLSPQQVVRRHILGSI
VQSEGSYVESLKRILQDYRNPLMEMEPKALSARKCQVVFFRVKEILHCHSMFQIALSS
RVAEWDSTEKIGDLFVASFSKSMVLDVYSDYVNNFTSAMSIIKKACLTKPAFLEFLKR
RQVCSPDRVTLYGLMVKPIQRFPQFILLLQDMLKNTPRGHPDRLSLQLALTELETLAE
KLNEQKRLADQVAEIQQLTKSVSDRSSLNKLLTSGQRQLLLCETLTETVYGDRGQLIK
SKERRVFLLNDMLVCANINFKGQLEISSLVPLGPKYVVKWNTALPQVQVVEVGQDGGT
YDKDNVLIQHSGAKKASASGQAQNKVYLGPPRLFQELQDLQKDLAVVEQITLLISTLH
GTYQNLNMTVAQDWCLALQRLMRVKEEEIHSANKCRLRLLLPGKPDKSGRPISFMVVF
ITPNPLSKISWVNRLHLAKIGLREENQPGWLCPDEDKKSKAPFWCPILACCIPAFSSR
ALSLQLGALVHSPVNCPLLGFSAVSTSLPQGYLWVGGGQEGAGGQVEIFSLNRPSPRT
VKSFPLAAPVLCMEYIPELEEEAESRDESPTVADPSATVHPTICLGLQDGSILLYSSV
DTGTQCLVSCRSPGLQPVLCLRHSPFHLLAGLQDGTLAAYPRTSGGVLWDLESPPVCL
TVGPGPVRTLLSLEDAVWASCGPRVTVLEATTLQPQQSFEAHQDEAVSVTHMVKAGSG
VWMAFSSGTSIRLFHTETLEHLQEINIATRTTFLLPGQKHLCVTSLLICQGLLWVGTD
QGVIVLLPVPRLEGIPKITGKGMVSLNGHCGPVAFLAVATSILAPDILRSDQEEAEGP
RAEEDKPDGQAHEPMPDSHVGRELTRKKGILLQYRLRSTAHLPGPLLSMREPAPADGA
ALEHSEEDGSIYEMAFFLKAKNTKPHNCLASPVSLVWALAGLQGSHTPLLEGCVRVSV
CECVGWCVNIYK"
BASE COUNT 641 a 1009 c 979 g 603 t
ORIGIN
1 aagaatgtta cccagcagct cgtgggggaa gaggaagctg cgaggcaggc tggggtgggt
61 gcggagtgat gcccggggcg tgatgtgggc ccggcaggag ggtctgcacc acccccaccc
121 ccacgccgtc atccgctgtc cctcctcctc ctcctcctct gtctcctgct caggtgactc
181 ggaggaggag gacatggggc tcctggaggt cagcgtttcg gacatcaagc ccccagcccc
241 agagctgggc cccatgccag agggcctgag ccctcagcag gtggtccgga ggcatatcct
301 gggctccatc gtgcagagcg aaggcagcta cgtggagtct ctgaagcgga tactccagga
361 ctaccgcaac cccctgatgg agatggagcc caaggcgctg agcgcccgca agtgccaggt
421 ggtgttcttc cgcgtgaagg agatcctgca ctgccactcc atgttccaga tcgccctgtc
481 ctcccgcgtg gctgagtggg attccaccga gaagatcggg gacctcttcg tggcctcgtt
541 ttccaagtcc atggtgctag atgtgtacag tgactacgtg aacaacttca ccagtgccat
601 gtccatcatc aagaaggcct gcctcaccaa gcctgccttc ctcgagttcc tcaagcgacg
661 gcaggtgtgc agcccagacc gtgtcaccct ctacgggctg atggtcaagc ccatccagag
721 gttcccacag ttcatactcc tgcttcagga catgctgaag aacaccccca ggggccatcc
781 ggacaggctg tcgctgcagc tggccctcac agagctggag acgctggctg agaagctgaa
841 cgagcagaag cggctggctg accaggtggc tgagatccag cagctgacca agagcgtcag
901 tgaccgcagc agcctcaaca agctgttgac ctcaggccag cggcagctgc tcctgtgtga
961 gacgttgacg gagaccgtgt acggtgaccg cgggcagcta attaagtcca aggagcgtcg
1021 ggtcttcctg ctcaacgaca tgcttgtctg tgccaacatc aacttcaagg gccagctgga
1081 gatcagcagc ctggtgcccc tggggcccaa gtatgtggtg aagtggaaca cggcgctgcc
1141 ccaggtgcag gtggtggagg tgggccagga cggtggcacc tatgacaagg acaatgtgct
1201 catccagcac tcaggcgcca agaaggcctc tgcctcaggg caggctcaga ataaggtgta
1261 cctcggcccc ccacgcctct tccaggagct gcaggacctg cagaaggacc tggccgtggt
1321 ggagcagatc acgcttctca tcagcacgct gcacggcacc taccagaacc tgaacatgac
1381 tgtggctcaa gactggtgcc tggccctgca gaggctgatg cgggtgaagg aggaagagat
1441 ccactcggcc aacaagtgcc gtctcaggct cctgcttcct gggaaacccg acaagtccgg
1501 ccgccccatt agcttcatgg tggttttcat cacccccaac cccctgagca agatttcctg
1561 ggtcaacagg ttacatttgg ccaaaatcgg actccgggag gagaaccagc caggctggct
1621 atgcccggat gaggacaaga agagcaaagc cccattctgg tgcccgatcc tggcctgctg
1681 catccctgcc ttctcctccc gggcactcag cctgcagctt ggggccctgg tccacagtcc
1741 tgtcaactgt cccctgctgg gtttctcagc agtcagcacc tcccttccac agggctacct
1801 ctgggtcggg ggcggacagg aaggcgcagg gggccaggtg gaaatctttt ccttgaaccg
1861 gccctcgccc cgcaccgtca agtccttccc actggcagcc cctgtgctct gcatggagta
1921 tatcccggag ctggaggagg aggcggagag cagagacgag agcccgacag ttgctgaccc
1981 ctcggccacg gtgcatccaa ccatctgcct cgggctccag gatggcagca tcctcctcta
2041 cagcagtgtg gacactggca cccagtgcct ggtgagctgc aggagcccag gtctgcagcc
2101 tgtactctgc ctgcgacaca gccccttcca cctgctcgct ggcctgcagg atgggaccct
2161 tgctgcttac cctcggacca gcggaggtgt cctgtgggac ctggagagcc ctcccgtgtg
2221 cctgactgtg gggcccgggc ctgtccgcac cctgttgagc ctggaggatg ccgtgtgggc
2281 cagctgtggg ccccgggtca ctgtcctgga agccaccacc ctgcagcctc agcaaagctt
2341 cgaggcgcac caggacgagg cagtgagcgt gacacacatg gtgaaggcgg gcagcggcgt
2401 ctggatggcc ttctcctccg gcacctccat ccgcctcttc cacactgaga ccctggagca
2461 tctgcaagag atcaacatcg ccaccaggac caccttcctc ctgccaggcc agaagcactt
2521 gtgtgtcacc agcctcctga tctgccaggg tctgctctgg gtgggcactg accagggtgt
2581 catcgtcctg ctgcccgtgc ctcggctgga aggcatcccc aagatcacag ggaaaggcat
2641 ggtctcactc aatgggcact gtgggcctgt ggccttcctg gctgtggcta ccagcatcct
2701 ggcccctgac atcctgcgga gtgaccagga ggaggctgag gggccccggg ctgaggagga
2761 caagccagac gggcaggcac acgagcccat gcccgacagc cacgtgggcc gagagctgac
2821 ccgcaagaag ggcatcctct tgcagtaccg cctgcgctcc accgcacacc tcccgggccc
2881 gctgctctcc atgcgggagc cggcgcctgc tgatggcgca gctttggagc acagcgagga
2941 ggacggctcc atttacgaaa tggccttctt tttaaaagca aaaaacacaa aacctcacaa
3001 ctgcctggca agcccagtat cacttgtttg ggccctagcg ggactccaag gcagccacac
3061 gcccctcctg gaagggtgtg tgcgtgtgag tgtgtgcgag tgtgtgggct ggtgtgtgaa
3121 tatctataaa taagtatata tggtgtatat tatatgtgta taaataaagt ctgtacatat
3181 tggagctctg ggagatgctg gaataaaaga caagagttac atctggactt gg
//