LOCUS AK300174 2007 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53189 complete cds, highly similar to Homo
sapiens Rho guanine nucleotide exchange factor (GEF) 10-like,
transcript variant 1, mRNA.
ACCESSION AK300174
VERSION AK300174.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2007)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2007
/clone="PLACE6007021"
/clone_lib="PLACE6"
/db_xref="H-InvDB:HIT000494798"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 417..1883
/codon_start=1
/note="highly similar to Homo sapiens Rho guanine
nucleotide exchange factor (GEF) 10-like, transcript
variant 1, mRNA"
/protein_id="BAG61954.1"
/transl_table=1
/translation="MTQLMKAAKSGTKDGLEKTRMAVMRKVSFLHRKDVLGDSEEEDM
GLLEVSVSDIKPPAPELGPMPEGLSPQQVVRRHILGSIVQSEGSYVESLKRILQDYRN
PLMEMEPKALSARKCQVVFFRVKEILHCHSMFQIALSSRVAEWDSTEKIGDLFVASFS
KSMVLDVYSDYVNNFTSAMSIIKKACLTKPAFLEFLKRRQVCSPDRVTLYGLMVKPIQ
RFPQFILLLQDMLKNTPRGHPDRLSLQLALTELETLAEKLNEQKRLADQVAEIQQLTK
SVSDRSSLNKLLTSGQRQLLLCETLTETVYGDRGQLIKSKERRVFLLNDMLVCANINF
KPANHRGQLEISSLVPLGPKYVVKWNTALPQVQVVEVGQDGGTYDKDNVLIQHSGAKK
ASASGQAQNKVYLGPPRLFQELQDLQKDLAVVEQITLLISTLHGTYQNLNMTVAQDWC
LALQRLMRVKEEEIHSANKCRLRLLLPGKPDKGTWSDM"
BASE COUNT 423 a 610 c 636 g 338 t
ORIGIN
1 acaggctctc tgtctgctct tcttttgcag acccagaccc agcagctgct ccacccggca
61 caggggtgcc agcctgggtg agcaatgggg atgcagcgga cgcagccttc tccggggccc
121 ggcactccag ctggaagcgg aagagttccc gtcgcattga ccggttcact ttccccgccc
181 tggaagagga tgtgatttat gacgacgtcc cctgcgagag cccagatgcg catcagcccg
241 gggcagagag gaacctgctc tacgaggatg cgcaccgggc tggggcccct cggcaggcgg
301 aggacctagg ctggagctcc agtgagttcg agagctacag cgaggactcg ggggaggagg
361 ccaagccgga ggtcgaggtc gagcccgcca agcaccgagt gtccttccag cccaagatga
421 cccagctcat gaaggccgcc aagagcggga ccaaggatgg gctggagaag acacggatgg
481 ccgtgatgcg caaagtctcc ttcctgcaca ggaaggacgt cctcggtgac tcggaggagg
541 aggacatggg gctcctggag gtcagcgttt cggacatcaa gcccccagcc ccagagctgg
601 gccccatgcc agagggcctg agccctcagc aggtggtccg gaggcatatc ctgggctcca
661 tcgtgcagag cgaaggcagc tacgtggagt ctctgaagcg gatactccag gactaccgca
721 accccctgat ggagatggag cccaaggcgc tgagcgcccg caagtgccag gtggtgttct
781 tccgcgtgaa ggagatcctg cactgccact ccatgttcca gatcgccctg tcctcccgcg
841 tggctgagtg ggattccacc gagaagatcg gggacctctt cgtggcctcg ttttccaagt
901 ccatggtgct agatgtgtac agtgactacg tgaacaactt caccagtgcc atgtccatca
961 tcaagaaggc ctgcctcacc aagcctgcct tcctcgagtt cctcaagcga cggcaggtgt
1021 gcagcccaga ccgtgtcacc ctctacgggc tgatggtcaa gcccatccag aggttcccac
1081 agttcatact cctgcttcag gacatgctga agaacacccc caggggccat ccggacaggc
1141 tgtcgctgca gctggccctc acagagctgg agacgctggc tgagaagctg aacgagcaga
1201 agcggctggc tgaccaggtg gctgagatcc agcagctgac caagagcgtc agtgaccgca
1261 gcagcctcaa caagctgttg acctcaggcc agcggcagct gctcctgtgt gagacgttga
1321 cggagaccgt gtacggtgac cgcgggcagc taattaagtc caaggagcgt cgggtcttcc
1381 tgctcaacga catgcttgtc tgtgccaaca tcaacttcaa gcctgccaac cacaggggcc
1441 agctggagat cagcagcctg gtgcccctgg ggcccaagta tgtggtgaag tggaacacgg
1501 cgctgcccca ggtgcaggtg gtggaggtgg gccaggacgg tggcacctat gacaaggaca
1561 atgtgctcat ccagcactca ggcgccaaga aggcctctgc ctcagggcag gctcagaata
1621 aggtgtacct cggcccccca cgcctcttcc aggagctgca ggacctgcag aaggacctgg
1681 ccgtggtgga gcagatcacg cttctcatca gcacgctgca cggcacctac cagaacctga
1741 acatgactgt ggctcaagac tggtgcctgg ccctgcagag gctgatgcgg gtgaaggagg
1801 aagagatcca ctcggccaac aagtgccgtc tcaggctcct gcttcctggg aaacccgaca
1861 aaggcacgtg gtcagacatg taaggtgtct aagacctgtg gacagctgac cgctgaccag
1921 gtggagtcac agcctgcaga ggctctgccg ggcacaaagc cagaggctcc tggagccagg
1981 ctggggagcg tggctcagcc accctgg
//