LOCUS AK300135 2567 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ61659 complete cds, highly similar to
Probable ATP-dependent RNA helicase DDX27 (EC 3.6.1.-).
ACCESSION AK300135
VERSION AK300135.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2567)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2567
/clone="PLACE5000481"
/clone_lib="PLACE5"
/db_xref="H-InvDB:HIT000494759"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 34..2331
/codon_start=1
/note="highly similar to Probable ATP-dependent RNA
helicase DDX27 (EC 3.6.1.-)"
/protein_id="BAH13221.1"
/transl_table=1
/translation="MLADLGLIGTIGEDDEVPVEPESDSGDEEEEGPIVLGRRQKALG
KNRSADFNPDFVFTEKEGTYDGSWALADVMSQLKKKRAATTLDEKIEKVRKKRKTEDK
EAKSGKLEKEKEAKEGSEPKEQEDLQENDEEGSEDEASETDYSSADENILTKADTLKV
KDRKKKKKKGQEAGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACI
PVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAPVTRVLVLVPTRELGIQVHS
VTRQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSI
EVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRI
FVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHI
LLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFT
MPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVIL
KFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWFQT
KEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAER
LAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRAGPSFEE
RKQLGLPHQRRGGNFKSKSRYKRRK"
BASE COUNT 730 a 598 c 736 g 503 t
ORIGIN
1 gtgctcgctt ccggaagtgg cttctgcgac aacatgcttg cggacctcgg cttaatcgga
61 accataggcg aggatgacga ggtgccggtg gagcccgagt ctgactccgg ggacgaggaa
121 gaggaggggc ccattgtgct gggcagacga caaaaagctt tggggaagaa ccgcagtgct
181 gatttcaacc ctgatttcgt tttcactgag aaggagggga cgtacgatgg cagctgggcc
241 ctggctgatg tcatgagcca actcaagaag aagagggcag ccactacatt agatgagaag
301 attgagaaag ttcgaaagaa aaggaaaaca gaggataaag aagccaagtc tgggaagttg
361 gaaaaggaga aagaagcaaa ggaaggctct gaaccaaagg agcaggaaga ccttcaagag
421 aatgatgagg aaggctcaga agatgaagcc tcggagactg actactcatc agctgatgag
481 aacatcctca ccaaagcaga tacactcaaa gtaaaggatc ggaagaagaa gaagaagaaa
541 ggacaggaag caggaggatt ttttgaagat gcatctcagt acgatgaaaa cctctcgttc
601 caggacatga acctttcccg ccctcttctg aaggccatta cagccatggg cttcaagcag
661 cccaccccga tccagaaggc gtgcatacct gtgggtctat tggggaagga catctgtgcc
721 tgtgcagcca ctgggacagg taaaactgcc gcctttgccc tgcctgtttt ggagcgtctg
781 atttataaac cccgccaggc tccagtcacc cgcgtgctgg tgctagtgcc cacccgagag
841 ctgggcatcc aggtgcactc tgtcaccaga cagctggccc agttctgcaa catcaccacc
901 tgcctggctg tgggcggctt ggatgtgaag tctcaggaag cagctcttcg ggcagcgcct
961 gacatcctca tcgccacccc aggccggctc atcgatcacc tccacaactg cccttccttc
1021 cacctgagca gcatcgaggt gctcatcctg gacgaggctg acaggatgct ggatgagtac
1081 tttgaggagc agatgaagga gatcatccga atgtgttccc accaccgcca gaccatgctc
1141 ttctcggcca ccatgacaga cgaggtgaaa gatctggctt ctgtctcctt gaagaatcct
1201 gtccggatat ttgtgaacag caacacagat gtggctccct ttctgcggca ggagttcatc
1261 cggatccggc ctaatcgtga aggagaccgg gaagccatcg tggcagcttt gttgacgagg
1321 accttcactg accatgtgat gctgttcacg caaaccaaga agcaggccca ccgcatgcac
1381 atcctcctgg ggctcatggg gctgcaggtg ggtgagctcc atggcaactt gtcacagacg
1441 cagcggctgg aggccctccg gcgttttaag gatgaacaga ttgacatcct cgtggccact
1501 gatgtggcag cccgtggact tgacattgag ggggtcaaaa cggtaatcaa cttcacaatg
1561 cctaatacca tcaaacatta tgtccaccgg gtggggcgaa cagcacgtgc tggcagggct
1621 gggcgctcag tctctctggt gggagaagat gagcggaaga tgctgaagga gattgtaaaa
1681 gctgccaagg cccctgtgaa ggccaggata cttccccaag atgtcatcct caaattccgg
1741 gacaagattg agaaaatgga gaaagatgtg tatgcagttc tgcagctaga ggcggaggaa
1801 aaagagatgc agcagtcaga agcccagatc aatacagcaa agcggctcct ggagaagggg
1861 aaggaggcag tggtccaaga gcccgagagg agctggttcc agaccaaaga agagaggaag
1921 aaggagaaaa ttgccaaagc tctgcaggaa tttgacttgg ccttaagagg aaagaagaaa
1981 aggaagaagt ttatgaagga tgccaaaaaa aagggggaga tgacagcaga ggaaaggtct
2041 cagtttgaaa tcctcaaggc gcagatgttt gctgaacggc tagcgaagag gaatcgcaga
2101 gccaagcggg cccgagcaat gcccgaggag gagccagtga gaggtcctgc caagaagcaa
2161 aagcagggga agaaatctgt atttgatgaa gaactcacca acacaagcaa gaaggccctg
2221 aaacagtatc gagctggccc ttcctttgaa gaaaggaaac agttgggctt gccccaccag
2281 agacgaggag gaaactttaa atctaaatcc agatacaaga ggaggaagta gctgtcgtgg
2341 cctgaagaaa ttcatggggg cagcccttaa atcccttccc tgtgggaagt catcctggct
2401 ggtctgtctt ttctccattt gtttaaaaaa aaaacaaaaa caaaaaacaa cactttggtg
2461 tggtggtatg gtacgtagct attttcctaa gcatgtctgt caatctccct tcttgctgat
2521 tagctttcat atgactatat taaatggaag tatttttggg aaaagag
//