LOCUS AK300128 2135 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50207 complete cds, highly similar to
Pappalysin-1 precursor (EC 3.4.24.79).
ACCESSION AK300128
VERSION AK300128.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2135)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2135
/clone="PLACE3000357"
/clone_lib="PLACE3"
/db_xref="H-InvDB:HIT000494752"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 277..1977
/codon_start=1
/note="highly similar to Pappalysin-1 precursor (EC
3.4.24.79)"
/protein_id="BAG61919.1"
/transl_table=1
/translation="MDCNYERFNFDGGECCDPEITNVTQTCFDPDSPHRAYLDVNELK
NILKLDGSTHLNIFFAKSSEEELAGVATWPWDKEALMHLDDDCTDSFTPNQVARMHCY
LDLVYQGWQPSRKPAPVALAPQVLGHTTDSVTLEWFPPIDGHFFEREWGSACHLCLEG
RILVQYASNASSPMPCSPSGHWSPREAEGHPDVEQPCKSSVRTWSPNSAVNPHTVPPA
CPEPQGCYLELEFLYPLVPESLTIWVTFVSTDWDSSGAVNDIKLLAVSGKNISLGPQN
VFCDVPLTIRLWDVGEEMYGIQIYTLDEHLEINAAMLTSTADTPLCLQCKPLKYKVVR
DPPLQMDVASILHLNRKFVDMDLNLGSVYQYWVITISGTEESEPSPAVTYIHGSGYCG
DGIIQKDQGEQCDDMNKINGDGCSLFCRQEVSFNCIDEPSRCYFHDGDGVCEEFEQKT
SIKDCGVYTPQGFLDQWASNASVSHQDQQCPGWVIIGQPAASQVCRTKVIDLSEGISA
CLVPLHHQLPIFPAGSDHFLAPGVFFSTNGCRSCHCPPGDGWDILWGPKAGDHQRAAA
"
BASE COUNT 506 a 596 c 544 g 489 t
ORIGIN
1 aacccgacgg tgacgcgcga gcaggtggac ttccagcacc atcagctggc tgaggccttc
61 aagcaataca acatctcctg ggagctggac gtgctggagg tgagcaactc ctcccttcgc
121 cgccgtctca tcctggccaa ctgtgacatc agcaagattg gggatgagaa ctgtgacccc
181 gagtgcaacc acacgctgac gggccacgac ggcggggatt gccgccacct gcgccaccct
241 gccttcgtga agaagcagca caacggggtg tgtgacatgg actgcaacta tgaacggttc
301 aactttgatg gtggagagtg ctgtgaccct gaaatcacca atgtcactca gacttgcttt
361 gaccccgact ctccacacag agcctacttg gatgttaatg agctgaagaa cattcttaaa
421 ttggatggat caacacatct caatattttc tttgcaaaat cctcagagga ggagttggca
481 ggagtagcaa cttggccatg ggacaaggag gccctgatgc acttagatga cgactgtacg
541 gactccttca cgcccaatca agtcgccaga atgcactgtt acctggacct ggtctaccag
601 ggctggcagc cctccaggaa accagcgcct gttgccctcg ccccccaagt tctgggccac
661 acaacggact ctgtgacact ggagtggttc ccacctatag atggccattt ctttgaaaga
721 gaatggggat cagcatgtca tctttgcctg gaagggagaa tcctggtgca gtatgcttcc
781 aacgcttcct ccccaatgcc ctgcagccca tcaggacact ggagccctcg tgaagcagaa
841 ggtcatcctg atgttgaaca gccctgtaag tccagtgtcc gcacctggag cccaaattca
901 gctgtcaacc cacacacggt tcctccagcc tgccctgagc ctcaaggctg ctacctcgag
961 ctggagttcc tctacccctt ggtccctgag tctctgacca tttgggtgac ctttgtctcc
1021 actgactggg actctagtgg agctgtcaat gacatcaaac tgttggctgt cagtgggaag
1081 aacatctccc tgggtcctca gaatgtcttc tgtgatgtcc cactgaccat cagactctgg
1141 gacgtgggcg aggagatgta tggcatccaa atctacacgc tggatgagca cctggagatc
1201 aatgctgcca tgttgacctc cactgcagac accccactct gtctacagtg taagcccctg
1261 aagtataagg tggtccggga ccctcctctc cagatggatg tggcctccat cctacatctc
1321 aataggaaat tcgtagacat ggatctaaat cttggcagtg tgtaccagta ttgggtcata
1381 actatttcag gaactgaaga gagtgagcca tcacctgctg tcacatacat ccatggaagt
1441 gggtactgtg gcgatggcat tatacaaaaa gaccaaggtg aacaatgcga cgacatgaat
1501 aagatcaatg gtgatggctg ctcccttttc tgccgacaag aagtctcctt caattgtatt
1561 gatgaaccca gccggtgcta tttccatgat ggtgatgggg tatgtgagga gtttgaacaa
1621 aaaaccagca ttaaggactg tggtgtctac acgccccagg gattcctgga tcagtgggca
1681 tccaatgctt cagtatctca tcaagaccag caatgcccag gctgggtcat catcggacag
1741 cctgcagcat cccaggtgtg tcgaaccaag gtgatagatc tcagtgaagg catttcagca
1801 tgcctggtac ccttgcacca tcagctaccc atattcccag ctggctcaga ccactttttg
1861 gctccgggcg tatttttctc aaccaatggt tgccgcagct gtcattgtcc acctggtgac
1921 ggatgggaca tattatgggg accaaaagca ggagaccatc agcgtgcagc tgcttgatac
1981 caaagatcag agccacgatc taggcctcca tgtcctgagc tgcaggaaca atcccctgat
2041 tatccctgtg gtccatgacc tcagccagcc cttctaccac agccaggcgg tacgtgtgag
2101 cttcagttcg cccctggtcg ccatctcggg ggtgg
//