LOCUS AK300121 1220 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60029 complete cds, highly similar to
Keratin, type II cuticular Hb3.
ACCESSION AK300121
VERSION AK300121.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1220)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1220
/clone="PLACE1006341"
/clone_lib="PLACE1"
/db_xref="H-InvDB:HIT000494745"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 349..1116
/codon_start=1
/note="highly similar to Keratin, type II cuticular Hb3"
/protein_id="BAG61913.1"
/transl_table=1
/translation="MEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALK
KDVDCAYLRKSDLEANVEALTQEIDFLRRLYEEEIRVLQSHISDTSVVVKMDNSRDLN
MHCVITEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRM
IQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLI
REYQEVMNSKLAWTLRSPPTGACWRARSRGCVRALVL"
BASE COUNT 269 a 339 c 416 g 196 t
ORIGIN
1 gcgggggctt ccgcgccggc tcctgcggac gcagcttcgg ctaccgctcc gggggcgtgt
61 gcggacccag ccccccatgc atcgccaccg tgtcggtcaa cgagagcctc ctcacgcccc
121 tcaacctgga gatcgacccc aacgcacagt gcgtgaagca ggaggagaag gagcagatca
181 agtccctcaa cagcaggttc gcggccttca tggacaaggt gcacttcctg gagcagcaga
241 acaagctgct ggagacaaag ctgcagttct tccagaaccg tgagtgctgc gagagcaacc
301 tggagcctct gtttgagggc tacaactctg cgacgggagg ccgagtgcat ggaagccgac
361 agtgggaggc tggcctcaga gctcaaccac gtgcaggagg tgctggaggg ttacaagaag
421 aagtatgaag aagaggtggc actaagggcc actgctgaga atgaatttgt ggccttgaag
481 aaggatgtgg actgtgccta cctgcgcaaa tcagacctag aggccaacgt ggaggccctg
541 acccaggaga tcgacttcct gaggcggcta tatgaggagg agatccgcgt tctccagtcg
601 cacatctcag acacctcagt ggtcgtcaag atggacaaca gtcgggacct gaacatgcac
661 tgtgtcatca ctgagatcaa agctcagtac gatgacattg ccacccgcag ccgggccgag
721 gccgagtcct ggtaccgcag caagtgtgag gagatgaagg ccacggtgat caggcacggg
781 gagaccctgc gccgcaccaa ggaggagatc aacgagctga accgcatgat ccagaggctg
841 acagccgagg tggagaacgc caagtgccag aactccaagc tggaggccgc ggtggcccag
901 tctgagcagc agggtgaggc ggccctcagt gatgcccgct gcaagctggc cgagctggag
961 ggtgccctgc agaaggccaa gcaggacatg gcctgcctga tcagggagta ccaggaggtg
1021 atgaactcca agctggcctg gactttgaga tcgccaccta caggcgcctg ctggagggcg
1081 aggagcagag gttgtgtgag ggcgttggtg ctgtaaatgt ctgtgtcagc agctcccgcg
1141 gcggggtctt atgcggggac ctgtgcgtgt cgggctcccg gccggtgacg ggcagcgtct
1201 gcagtgcccc ctgcagcggg
//