LOCUS AK300050 2112 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54507 complete cds, highly similar to Heat
shock 70 kDa protein 4.
ACCESSION AK300050
VERSION AK300050.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2112)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2112
/cell_type="peripheral blood mononuclear cells
(HPBMC5939)"
/clone="PEBLM2008569"
/clone_lib="PEBLM2"
/db_xref="H-InvDB:HIT000494674"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, peripheral blood mononuclear
cells"
/organism="Homo sapiens"
CDS 23..2077
/codon_start=1
/note="highly similar to Heat shock 70 kDa protein 4"
/protein_id="BAG61859.1"
/transl_table=1
/translation="MDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVF
VDMGHSAYQVSVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKS
KIRALLRLSQECEKLKKLMSANASDLPLSIECFMNDVDVSGTMNRGKFLEMCNDLLAR
VEPPLRSVLEQTKLKKEDIYAVEIVGGATRIPAVKEKISKFFGKELSTTLNADEAVTR
GCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVFSKNHAAPFSKVL
TFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIF
SVSSASLVEVHKSEENEEPMETDQNAKEEEKMQVDQEEPHVEEQQQQTPAENKAESEE
METSQAGSKDKKMDQPPQAKKAKVKTSTVDLPIENQLLWQIDREMLNLYIENEGKMIM
QDKLEKERNDAKNAVEEYVYEMRDKLSGEYEKFVSEDDRNSFTLKLEDTENWLYEDGE
DQPKQVYVDKLAELKNLGQPIKIRFQESEERPKLFEELGKQIQQYMKIISSFKNKEDQ
YDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCS
PIISKPKPKVEPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMD
ID"
BASE COUNT 726 a 368 c 495 g 523 t
ORIGIN
1 tgatgcagaa agacgatcag tgatggatgc aacacagatt gctggtctta attgcttgcg
61 attaatgaat gaaaccactg cagttgctct tgcatatgga atctataagc aggatcttcc
121 tgccttagaa gagaaaccaa gaaatgtagt ttttgtagac atgggccact ctgcttatca
181 agtttctgta tgtgcattta atagaggaaa actgaaagtt ctggccactg catttgacac
241 gacattggga ggtagaaaat ttgatgaagt gttagtaaat cacttctgtg aagaatttgg
301 gaagaaatac aagctagaca ttaagtccaa aatccgtgca ttattacgac tctctcagga
361 gtgtgagaaa ctcaagaaat tgatgagtgc aaatgcttca gatctccctt tgagcattga
421 atgttttatg aatgatgttg atgtatctgg aactatgaat agaggcaaat ttctggagat
481 gtgcaatgat ctcttagcta gagtggagcc accacttcgt agtgttttgg aacaaaccaa
541 gttaaagaaa gaagatattt atgcagtgga gatagttggt ggtgctacac gaatccctgc
601 ggtaaaagag aagatcagca aatttttcgg taaagaactt agtacaacat taaatgctga
661 tgaagctgtc actcgaggct gtgcattgca gtgtgccatc ttatcgcctg ctttcaaagt
721 cagagaattt tctatcactg atgtagtacc atatccaata tctctgagat ggaattctcc
781 agctgaagaa gggtcaagtg actgtgaagt cttttccaaa aatcatgctg ctcctttctc
841 taaagttctt acattttata gaaaggaacc tttcactctt gaggcctact acagctctcc
901 tcaggatttg ccctatccag atcctgctat agctcagttt tcagttcaga aagtcactcc
961 tcagtctgat ggctccagtt caaaagtgaa agtcaaagtt cgagtaaatg tccatggcat
1021 tttcagtgtg tccagtgcat ctttagtgga ggttcacaag tctgaggaaa atgaggagcc
1081 aatggaaaca gatcagaatg caaaggagga agagaagatg caagtggacc aggaggaacc
1141 acatgttgaa gagcaacagc agcagacacc agcagaaaat aaggcagagt ctgaagaaat
1201 ggagacctct caagctggat ccaaggataa aaagatggac caaccacccc aagccaagaa
1261 ggcaaaagtg aagaccagta ctgtggacct gccaatcgag aatcagctat tatggcagat
1321 agacagagag atgctcaact tgtacattga aaatgagggt aagatgatca tgcaggataa
1381 actggagaag gagcggaatg atgctaagaa cgcagtggag gaatatgtgt atgaaatgag
1441 agacaagctt agtggtgaat atgagaagtt tgtgagtgaa gatgatcgta acagttttac
1501 tttgaaactg gaagatactg aaaattggtt gtatgaggat ggagaagacc agccaaagca
1561 agtttatgtt gataagttgg ctgaattaaa aaatctaggt caacctatta agatacgttt
1621 ccaggaatct gaagaacgac caaaattatt tgaagaacta gggaaacaga tccaacagta
1681 tatgaaaata atcagctctt tcaaaaacaa ggaggaccag tatgatcatt tggatgctgc
1741 tgacatgaca aaggtagaaa aaagcacaaa tgaagcaatg gagtggatga ataacaagct
1801 aaatctgcag aacaagcaga gtttgaccat ggatccagtt gtcaagtcaa aagagattga
1861 agctaaaatt aaggagctga caagtacttg tagccctata atttcaaagc ccaaacccaa
1921 agtggaacct ccaaaagagg aacaaaaaaa tgcagagcag aatggaccag tggatggaca
1981 aggagacaac ccaggccccc aggctgctga gcagggtaca gacacagctg tgccttcgga
2041 ttcagacaag aagcttcctg aaatggacat tgattgattc caacacttgt ttctattaaa
2101 acagactatt at
//