LOCUS AK300045 1994 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ55591 complete cds, highly similar to Rho
guanine nucleotide exchange factor 3.
ACCESSION AK300045
VERSION AK300045.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1994)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1994
/cell_type="peripheral blood mononuclear cells
(HPBMC5939)"
/clone="PEBLM2007923"
/clone_lib="PEBLM2"
/db_xref="H-InvDB:HIT000494669"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, peripheral blood mononuclear
cells"
/organism="Homo sapiens"
CDS 113..1711
/codon_start=1
/note="highly similar to Rho guanine nucleotide exchange
factor 3"
/protein_id="BAH13198.1"
/transl_table=1
/translation="MRSERPMVWCCLFVRSQRKRKQSTQDEDAVSLCSLDISEPSNKR
VKPLSRVTSLANLIPPVKATPLKRFSQTLQRSISFRSESRPDILAPRPWSRNAAPSST
KRRDSKLWSETFDVCVNQMLTSKEIKRQEAIFELSQGEEDLIEDLKLAKKAYHDPMLK
LSIMTEQELNQIFGTLDSLIPLHEELLSQLRDVRKPDGSTEHVGPILVGWLPCLSSYD
SYCSNQVAAKALLDHKKQDHRVQDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLL
REILRHTPNDNPDQQHLEEAINIIQGIVAEINTKTGESECRYYKERLLYLEEGQKDSL
IDSSRVVCCHGELKNNRGVKLHVFLFQEVLVITRAVTHNEQLCYQLYRQPIPVKDLLL
EDLQDGEVRLGGSLRGAFSNNERIKNFFRVSFKNGSQSQTHSLQANDTFNKQQWLNCI
RQAKETVLCAAGQAGVLDSEGSFLNPTTGSRELQGETKLEQMDQSDSESDCSMDTSEV
SLDCERMEQTDSSCGNSRHGESNV"
BASE COUNT 540 a 501 c 491 g 462 t
ORIGIN
1 atgcagccca agtggcgggg aggagttcct tcagggaaac tatgctcagc cctgttcaga
61 gaatcctgtg cacagggctc cactgcagtt aggagccggt gagtccgggt ggatgaggtc
121 agagcgcccc atggtgtggt gctgcctctt tgtccgttcg cagcgaaaac ggaaacagag
181 cacccaagat gaagatgctg ttagcctttg cagtctcgac ataagtgagc ctagtaataa
241 acgggtcaaa cccctttccc gagtcacgtc gctagcaaac ctcatcccgc ccgtgaaggc
301 cacgccatta aagcgcttca gtcaaaccct gcagcgctcc attagcttcc gcagtgagag
361 ccgccctgac atcctcgccc cccgaccctg gtccagaaat gccgccccct cgagcacgaa
421 acggagagat agcaagctgt ggagtgagac cttcgatgtg tgcgtcaatc agatgcttac
481 atccaaggaa atcaaacgtc aggaggcgat ctttgagctt tcccaaggag aagaagactt
541 gatagaagac ttgaaattag caaaaaaggc ctatcatgac cccatgctga aactctccat
601 aatgacagaa caagagttga atcaaatttt tggaacactg gactctctaa ttcctctaca
661 tgaagagctc cttagtcagc ttcgagatgt taggaagcct gatggctcga ctgaacatgt
721 tggtcccatt ctcgtgggct ggctcccttg cctcagctcc tatgatagct actgcagcaa
781 tcaagtagcc gccaaagctc tgctggacca caaaaagcaa gatcaccgag tccaggattt
841 cctacagcga tgtttagaat ccccctttag ccgcaaacta gatctctgga atttcctcga
901 tattccaaga agccgcctgg taaaataccc tctgcttctc cgagaaatct tgaggcacac
961 accaaatgat aatccagatc agcagcactt ggaagaagct ataaatatca ttcagggaat
1021 tgtggcagaa atcaacacca agactggtga atctgaatgc cgctattata aggagcggct
1081 tctttacttg gaagaaggcc agaaagactc cctgatcgac agctctcgag tcgtgtgttg
1141 tcatggtgaa ctgaagaaca atcggggcgt gaaactgcat gttttcctgt tccaagaagt
1201 gcttgtgatc actcgagccg tcacccacaa tgagcagctt tgctaccagc tgtaccgtca
1261 gccaatcccc gtgaaagacc tcctgctgga agacctccag gatggagaag tgaggctggg
1321 tggctccctg cgaggggcat tcagcaacaa tgagagaatt aaaaacttct tcagagtcag
1381 tttcaaaaat ggatcccaaa gtcagaccca ctcgctacaa gccaatgaca ctttcaacaa
1441 acagcagtgg cttaactgta ttcgtcaagc caaagaaaca gttttgtgtg ctgccgggca
1501 agctggggtg cttgactccg agggatcgtt cctaaatccc accaccggga gcagagagct
1561 acagggagaa acaaaacttg agcagatgga ccaatcggac agtgagtcag actgtagtat
1621 ggacacgagt gaggtcagcc tcgactgtga gcgcatggaa cagacagact cttcctgtgg
1681 gaacagcagg catggtgaaa gtaacgtctg acagaagcat gtgcacttcg ggaagcaggc
1741 ctgcatctta cctgtacagt atttgcattc cacagatgga acggtttgga gaagcacttt
1801 ttcatacttt tgtgaaagta tacatgttgg cccagtctct cgtatctgta cctttgtccc
1861 tagtactgta actgccaatc tgtctgtgta agctggaatc tgtggcaact attaccctgt
1921 gttgtatttc ccaagtgtct ggatggatgg agaggtactc aaacaagtta ctttcagttg
1981 tcctgctgga tttt
//