LOCUS AK299413 2240 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53645 complete cds, highly similar to
Pyruvate kinase isozyme M1 (EC 2.7.1.40).
ACCESSION AK299413
VERSION AK299413.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2240)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2240
/clone="NTONG2003817"
/clone_lib="NTONG2"
/db_xref="H-InvDB:HIT000494037"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="tongue"
CDS 87..1622
/codon_start=1
/note="highly similar to Pyruvate kinase isozyme M1 (EC
2.7.1.40)"
/protein_id="BAG61395.1"
/transl_table=1
/translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
IIKGSGTAEVELKKGSTLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGL
ISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVD
MVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGD
LGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLD
GADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAM
AMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIF
PVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVV
PVP"
BASE COUNT 504 a 613 c 639 g 484 t
ORIGIN
1 acagcagctg cacgcgccgt ggctccggat ctcttcgtct ttgcagcgta gcccgagtcg
61 gtcagcgccg gaggacctca gcagccatgt cgaagcccca tagtgaagct gggactgcct
121 tcattcagac ccagcagctg cacgcagcca tggctgacac attcctggag cacatgtgcc
181 gcctggacat tgattcacca cccatcacag cccggaacac tggcatcatc tgtaccattg
241 gcccagcttc ccgatcagtg gagacgttga aggagatgat taagtctgga atgaatgtgg
301 ctcgtctgaa cttctctcat ggaactcatg agtaccatgc ggagaccatc aagaatgtgc
361 gcacagccac ggaaagcttt gcttctgacc ccatcatcaa gggcagcggc actgcggagg
421 tggagctgaa gaagggatcc actctcaaaa tcacgctgga taacgcctac atggaaaagt
481 gtgacgagaa catcctgtgg ctggactaca agaacatctg caaggtggtg gaagtgggca
541 gcaagatcta cgtggatgat gggcttattt ctctccaggt gaagcagaaa ggtgccgact
601 tcctggtgac ggaggtggaa aatggtggct ccttgggcag caagaagggt gtgaaccttc
661 ctggggctgc tgtggacttg cctgctgtgt cggagaagga catccaggat ctgaagtttg
721 gggtcgagca ggatgttgat atggtgtttg cgtcattcat ccgcaaggca tctgatgtcc
781 atgaagttag gaaggtcctg ggagagaagg gaaagaacat caagattatc agcaaaatcg
841 agaatcatga gggggttcgg aggtttgatg aaatcctgga ggccagtgat gggatcatgg
901 tggctcgtgg tgatctaggc attgagattc ctgcagagaa ggtcttcctt gctcagaaga
961 tgatgattgg acggtgcaac cgagctggga agcctgtcat ctgtgctact cagatgctgg
1021 agagcatgat caagaagccc cgccccactc gggctgaagg cagtgatgtg gccaatgcag
1081 tcctggatgg agccgactgc atcatgctgt ctggagaaac agccaaaggg gactatcctc
1141 tggaggctgt gcgcatgcag cacctgatag ctcgtgaggc tgaggcagcc atgttccacc
1201 gcaagctgtt tgaagaactt gtgcgagcct caagtcactc cacagacctc atggaagcca
1261 tggccatggg cagcgtggag gcttcttata agtgtttagc agcagctttg atagttctga
1321 cggagtctgg caggtctgct caccaggtgg ccagataccg cccacgtgcc cccatcattg
1381 ctgtgacccg gaatccccag acagctcgtc aggcccacct gtaccgtggc atcttccctg
1441 tgctgtgcaa ggacccagtc caggaggcct gggctgagga cgtggacctc cgggtgaact
1501 ttgccatgaa tgttggcaag gcccgaggct tcttcaagaa gggagatgtg gtcattgtgc
1561 tgaccggatg gcgccctggc tccggcttca ccaacaccat gcgtgttgtt cctgtgccgt
1621 gatggacccc agagcccctc ctccagcccc tgtcccaccc ccttccccca gcccatccat
1681 taggccagca acgcttgtag aactcactct gggctgtaac gtggcactgg taggttggga
1741 caccagggaa gaagatcaac gcctcactga aacatggctg tgtttgcagc ctgctctagt
1801 gggacagccc agagcctggc tgcccatcat gtggccccac ccaatcaagg gaagaaggag
1861 gaatgctgga ctggaggccc ctggagccag atggcaagag ggtgacagct tcctttcctg
1921 tgtgtactct gtccagttcc tttagaaaaa atggatgccc agaggactcc caaccctggc
1981 ttggggtcaa gaaacagcca gcaagagtta ggggccttag ggcactgggc tgttgttcca
2041 ttgaagccga ctctggccct ggcccttact tgcttctcta gctctctagg cctctccagt
2101 ttgcacctgt ccccaccctc cactcagctg tcctgcagca aacactccac cctccacctt
2161 ccattttccc ccactactgc agcacctcca ggcctgttgc tatagagcct acctgtatgt
2221 caataaacaa cagctgaagc
//