LOCUS AK299360 1781 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59046 complete cds.
ACCESSION AK299360
VERSION AK299360.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1781)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1781
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8007870"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493984"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 243..1685
/codon_start=1
/protein_id="BAG61355.1"
/transl_table=1
/translation="MQKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHKKTKITFEED
KVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDE
GYSTLSIPPEMLASYQSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDQEATGPRLSR
ELLDEKEPEVLQDSLDRCYSTPSGCLELTDSCQPYRSAFYVLEQQRVGLAIDMDEIEK
YQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSDYLELPDLGQPYSSAVYS
LEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSS
CLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKKRRRGRKEGE
DDNPPCPRLYGVLMEVEEPEVLQDSLDRCYSTPSMYFEQPDSFQHYRSVFYSFEEEHI
SFALYVDNRFFTLTVTSLHLVFQMGVIFPQ"
BASE COUNT 529 a 390 c 477 g 385 t
ORIGIN
1 gggagaagtt acgggaaggg agagatgcct cccgctcatt gaatgagcat ctccaggccc
61 tcctcactcc ggatgagccg gacaagtccc aggggcagga cctccaagaa cagctggctg
121 aggggtgtag actgacacag caccttgtcc aaaagctcag cccagaaaat gacaacgatg
181 acgatgaaga tgttcaagtt gaggtggctg agaaagtgca gaaatcgtct gcccccaggg
241 agatgcagaa ggctgaagaa aaggaagtcc ctgaggactc actggaggaa tgtgccatca
301 cttgttcaaa tagccatggc ccttatgact ccaaccagcc acataagaaa accaaaatca
361 catttgagga agacaaagtc gactcaactc tcattggctc atcctctcat gttgaatggg
421 aggatgctgt acacattatt ccagaaaatg aaagtgatga tgaggaagag gaagaaaaag
481 ggccagtgtc tcccaggaat ctgcaggagt ctgaagagga ggaagtcccc caggagtcct
541 gggatgaagg ttattcgact ctctcaattc ctcctgaaat gttggcctcg taccagtctt
601 acagcagcac atttcactca ttagaggaac agcaagtctg catggctgtt gacataggca
661 gacatcggtg ggatcaagtg aaaaaggagg accaagaggc aacaggtccc aggctcagca
721 gggagctgct ggatgagaaa gagcctgaag tcttgcagga ctcactggat agatgttatt
781 caactccttc aggttgtctt gaactgactg actcatgcca gccctacaga agtgcctttt
841 acgtattgga gcaacagcgt gttggcttgg ctattgacat ggatgaaatt gaaaagtacc
901 aagaagtgga agaagaccaa gacccatcat gccccaggct cagcagggag ctgctggatg
961 agaaagagcc tgaagtcttg caggactcac tggatagatg ttattcgact ccttcagatt
1021 atcttgaact gcctgactta ggccagccct acagcagtgc tgtttactca ttggaggaac
1081 agtaccttgg cttggctctt gacgtggaca gaattaaaaa ggaccaagaa gaggaagaag
1141 accaaggccc accatgcccc aggctcagca gggagctgct ggaggtagta gagcctgaag
1201 tcttgcagga ctcactggat agatgttatt caactccttc cagttgtctt gaacagcctg
1261 actcctgcca gccctatgga agttcctttt atgcattgga ggaaaaacat gttggctttt
1321 ctcttgacgt gggagaaatt gaaaagaagg ggaaggggaa gaaaagaagg ggaagaagat
1381 caaagaagaa aagaagaagg ggaagaaaag aaggggaaga tgacaaccca ccatgcccca
1441 ggctctacgg cgtgctgatg gaagtggaag agcctgaagt cttacaggac tcactggata
1501 gatgttattc gactccgtca atgtactttg aacaacctga ctcattccag cactacagaa
1561 gtgtgtttta ctcatttgag gaagagcata tcagcttcgc cctttacgtg gacaataggt
1621 tttttacttt gacggtgaca agtctccacc tggtgttcca gatgggagtc atattcccac
1681 aataagcagc tcttactaag ccgagagatg tcattcctgc aggcaggacc tataggcacg
1741 agaagatttg aatgaaagta cagttccatt tggaagccca g
//