LOCUS AK299353 3438 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54447 complete cds.
ACCESSION AK299353
VERSION AK299353.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3438)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3438
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8007125"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493977"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 104..3259
/codon_start=1
/protein_id="BAG61349.1"
/transl_table=1
/translation="MPGKLKVKIVAGRHLPVMDRASDLTDAFVEVKFGNTTFKTDVYL
KSLNPQWNSEWFKFEVDDEDLQDEPLQITVLDHDTYSANDAIGKVYIDIDPLLYSEAA
TVISGWFPIYDTIHGIRGEINVVVKVDLFNDLNRFRQSSCGVKFFCTTSIPKCYRAVI
IHGFVEELVVNEDPEYQWIDRIRTPRASNEARQRLISLMSGELQRKIGLKVLEMRGNA
VVGYLQCFDLEGESGLVVRAIGTACTLDKLSSPAAFLPACNSPSKEMKEIPFNEDPNP
NTHSSGPSTPLKNQTYSFSPSKSYSRQSSSSDTDLSLTPKTGMGSGSAGKEGGPFKAL
LRQQTQSALEQREFPFFTLTAFPPGFLVHVGGVVSARSVKLLDRIHNPDEPETRDAWW
AEIRQEIKSHAKALGCHAVVGYSESTSICEEVCILSASGTAAVLNPRFLQDGTVEGCL
EQRLEENLPTRCGFCHIPYDELNMPFPAHLTYCYNCRKQKVPDVLFTTIDLPTDATVI
GKGCLIQARLCRLKKKAQAEANATAISNLLPFMEYEVHTQLMNKLKLKGMNALFGLRI
QITVGENMLMGLASATGVYLAALPTPGGIQIAGKTPNDGSYEQHISHMQKKINDTIAK
NKELYEINPPEISEEIIGSPIPEPRQRSRLLRSQSESSDEVTELDLSHGKKDAFVLEI
DDTDAMEDVHSLLTDVPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTN
QALNKNFNDLCENLLKSLYFKLRSMIPCCLCHVNFTVSLPEDELIQVTVTAVAITFDK
NQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPSHPFPPAKEHLESASSNSGI
PAAQRATSVDYSSFADRCSSWIELIKLKAQTIRRGSIKTTMTVEKASPVGDGNFRNRS
APPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIA
EVFAMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEV
VSSQQPTTNCQSSCTEGEVTT"
BASE COUNT 1037 a 692 c 745 g 964 t
ORIGIN
1 agttaccccg cgcgcctccg cccctctccc gccggccccg cgctcggcgc agactcgttt
61 gtttgtttct gcaggagccc aagaagaggc cgaaaccgag accatgccag ggaagctgaa
121 ggtgaaaatc gtggccgggc gccatttgcc agtgatggac cgtgctagtg acctgactga
181 tgccttcgtg gaggtaaaat ttggtaatac cacctttaaa acagatgtgt accttaagtc
241 actcaaccct cagtggaact cggagtggtt taaatttgag gtggatgatg aagacttaca
301 agatgaacct ttacagatca cagttcttga ccatgatact tacagtgcaa atgatgccat
361 tggtaaagtg tacattgata ttgatccttt actgtatagt gaagctgcaa cagtcatctc
421 aggatggttt ccaatttatg acaccataca tggtatccgt ggggaaatca atgtagttgt
481 caaagtagac ctcttcaatg atttaaatcg atttaggcag tcatcatgtg gagtcaaatt
541 cttttgcaca acgtctattc caaaatgcta tagagctgtg ataattcatg gatttgtaga
601 agaacttgtg gtcaatgaag acccagaata tcagtggatt gatcgaattc gcacaccaag
661 ggcatcaaat gaggccagac agagactcat ttcgttaatg tcaggtgagc tgcagaggaa
721 gattggcttg aaagtacttg aaatgagagg aaatgcagtt gtggggtact tacagtgttt
781 cgatctggag ggcgagtctg ggttagtggt gcgagccata ggaacggcgt gtactctgga
841 taaattaagt agcccagcag cattccttcc tgcatgtaat tccccatcca aagaaatgaa
901 ggagattccc ttcaatgaag atcccaatcc caatactcac tcatcaggac cctcaacccc
961 tctgaaaaac caaacttatt ccttttcacc ttccaagtcc tacagtcgac agtcctcttc
1021 ttctgacaca gatttgagtt tgacacccaa aactggaatg ggaagtggta gtgctggaaa
1081 agaagggggg ccctttaaag ctcttttaag acaacagact caatcagcgt tggaacagag
1141 ggaatttcca ttttttacct tgacggcatt tcctcctgga ttccttgtac acgttggggg
1201 tgtagttagt gcacgttctg tgaagctttt ggatcgtatc cacaatcctg atgaaccaga
1261 aactcgagat gcatggtggg cagaaatcag acaagaaata aaatcacatg ctaaagcatt
1321 aggctgtcat gctgtagtgg gctacagtga atcaactagc atctgtgaag aggtctgcat
1381 tttatctgca tctggcacag cggctgtact gaatcctaga tttctgcagg atggcaccgt
1441 ggaaggctgt ttggaacaga ggcttgaaga aaatttgcct acacgttgtg gattttgtca
1501 tataccatat gatgaactga atatgccatt tccagctcat ctcacatatt gctataactg
1561 caggaaacaa aaagttcctg atgttctgtt tacaactata gacctcccaa cagatgcaac
1621 agttattgga aaaggttgtc ttattcaagc aaggttatgt cgcttaaaaa agaaagcaca
1681 ggcagaagca aatgctacag ctatcagtaa tctcttgcca tttatggaat atgaagtgca
1741 tactcagcta atgaataaac taaaactcaa aggaatgaat gctttgtttg gactaagaat
1801 tcagatcaca gtgggtgaaa atatgttgat gggcttagcg tctgccacag gtgtgtattt
1861 agcagcttta ccaactcctg gtggtattca gattgctggg aagactccta atgatggctc
1921 atatgaacaa cacatctctc atatgcagaa gaagataaat gacacaattg ctaaaaacaa
1981 agagttatat gaaatcaatc ctccagagat atctgaagag attataggat cacccatccc
2041 agaacctagg caacgctcaa gacttctaag atctcaatca gaaagctcgg atgaagttac
2101 agaattagac ctttcacatg ggaaaaaaga tgcttttgtt ttggagattg atgacacaga
2161 tgccatggaa gatgtccatt ctctacttac tgatgttcct cctccttcag gcttttatag
2221 ttgtaataca gaaattatgc ccggtataaa taattggacc tctgaaatac agatgttcac
2281 ttcagtaaga gtaatcagat taagcagcct caacctgact aatcaagctc tcaataagaa
2341 ctttaatgat ctctgtgaaa atttgctcaa gagcctgtac tttaaactac gatctatgat
2401 cccctgctgc ctttgccatg taaattttac agtatctctg cctgaagatg aattaattca
2461 ggttacagtc acggcagtcg caatcacttt tgacaaaaat caggctttac aaaccacaaa
2521 aacccctgtt gaaaagtcat tgcaaagagc ctcaacagat aatgaagaac ttttacaatt
2581 tccactggaa ctgtgttcag attcacttcc ttctcatcct tttccaccag ctaaagaaca
2641 cctggagagt gcaagttcta actcaggtat accagctgca cagagagcaa cgtcagttga
2701 ttacagttcc tttgcagaca gatgcagcag ctggatagag ctcattaaac tgaaagctca
2761 gaccataagg cgtggatcaa ttaagacaac catgacagtt gaaaaagcaa gtccagtggg
2821 tgatggaaat ttccggaatc gttctgctcc accttgtgca aattccacag ttggggttgt
2881 taagatgaca cctctttctt ttattcctgg agcaaaaata actaagtatc ttgggataat
2941 taacatgttt tttattcgag agactacttc tcttcgggag gaaggaggag tcagtggttt
3001 tctccatgct tttattgctg aagtgtttgc aatggtgaga gctcatgttg ctgcattagg
3061 agggaatgct gttgtctcct acataatgaa gcagtgtgtc ttcatggaga atccaaataa
3121 aaaccaggca cagtgtctta taaatgtaag tggtgacgca gtggtttttg ttcgtgaatc
3181 tgacttagaa gtggtgtcat ctcagcaacc tactaccaac tgccagtcat catgtactga
3241 aggcgaagtt acaacctgag gaaaattagg aaaaaagagc tcaactaaat gaaattcatc
3301 aatctctgtt attttgtcat taattatctt cttagactta aaattgaact tgagataaat
3361 aaggaagaat tgagtagatt tgatttgcct ggagtctttt ggaggcatga gaattttcta
3421 atcttgacat tttgtttg
//