LOCUS AK299344 1569 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ61356 complete cds.
ACCESSION AK299344
VERSION AK299344.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1569)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1569
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8006415"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493968"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 351..1091
/codon_start=1
/protein_id="BAG61345.1"
/transl_table=1
/translation="MKLNERSLAFYATCDAPVDNAGFLYKKGGRHAAYHRRWFVLRGN
MLFYFEDAASREPVGVIILEGCTVELVEAAEEFAFAVRFAGTRARTYVLAAESQDAME
GWVKALSRASFDYLRLVVRELEQQLAAVRGMALPQPQPQSLPLPPSLPSALAPVPSLP
SAPAPVPALPLPRRPSALPPKENGCAVWSTEATFRPGPEPPPPPPRRRASAPHGPLDM
APFARLHECYGQEIRALRGQWLSSRVQP"
BASE COUNT 247 a 547 c 526 g 249 t
ORIGIN
1 gactgtccgg ttgctcccgc ctccttccgt cggatccgcg ctctcccgcg gctgcgccgg
61 cccggccgcc caggagacaa agcgctgccg ccgccgtcgc cgccaccggg ccagtcccga
121 accatggccg ggccaccccg caacctcgcg cctcccgtcc cgtcgccgcc ctaggcctgc
181 gctgcgagat gcagaaacag aggcccagag agatgaatag cctgcctggg gtcccccagc
241 ccagatgctg cagagctgac atttgaatcc agagaagagc gagctgaggc tggagctgag
301 cagagggcag ggaggcccca ggctcccctc caggccccgc gattgccacc atgaagctga
361 acgagcgcag cctggccttc tacgccacct gtgacgcccc ggtggacaat gcaggcttcc
421 tgtacaagaa gggtgggcgg cacgcggcct accaccggcg ctggttcgtg ctgcgcggga
481 acatgctctt ctacttcgag gacgctgcca gccgtgagcc cgtgggcgtc atcatcctgg
541 agggctgcac tgtggagctg gtggaggccg ccgaggagtt cgccttcgct gtgcgctttg
601 cggggacccg ggcgcgcacc tacgtgctgg ccgctgagag tcaggatgcc atggagggct
661 gggtcaaggc cctgtcgcgt gccagcttcg actacctgcg gctggtggtg cgcgagctgg
721 agcagcagct ggcggctgta cgtggcatgg ccctgcccca gccccagccc cagtccctgc
781 ccttgccccc gtccctgccc tctgccctgg ccccagtccc atccctgcct tctgccccag
841 ccccggtccc agccctgccc ctgccccgcc ggcccagtgc cctcccgccc aaggagaatg
901 gctgcgctgt ctggagcact gaggccacct tcaggcctgg acccgagccc cctccaccac
961 cgcctcgccg ccgggcctcg gcaccccacg ggcccctgga catggccccc ttcgcccggc
1021 tgcacgagtg ctatggccag gagatccggg ccctgcgtgg ccagtggctc agcagccggg
1081 tccagccctg aggccaccag ggcgcctgtt ttagggggac acaagctcta tgccctctgg
1141 gacaggccgg gtcctggaga gactggggga gcagatcctg agggaaatca tgggcaaggc
1201 tggcccccag gccctgagtg gcctggtttt gggggatgca ggctctgaga aggtgctgga
1261 ttctgagtgg gcctttagga ccatgtgacc cagaaggcct acccttgagg ggaacgtcac
1321 gtgcaacact aaggagaatc cagggcctga gggggaggtt tcctgagaac tccggcccca
1381 ggctggcctg agctgccggg cagaggcctg tgctggcaac aggcattgcc gggcctgcag
1441 ctcagagggt cgctgggact gagggcgatc tgtggcctga aaagcaaatg cacagttagt
1501 gcagctcctg accgggcctt cagggtggac agagggagga tcggtcatgg cagccacacc
1561 tgggcctgg
//