LOCUS AK299341 2649 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50183 complete cds, highly similar to
Frizzled-6 precursor.
ACCESSION AK299341
VERSION AK299341.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2649)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2649
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8006337"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493965"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 265..2289
/codon_start=1
/note="highly similar to Frizzled-6 precursor"
/protein_id="BAG61342.1"
/transl_table=1
/translation="MKMAYNMTFFPNLMGHYDQSIAAVEMEHFLPLANLECSPNIETF
LCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKKLIDTFGIRWPEELECDRLQYCDETVP
VTFDPHTEFLGPQKKTEQVQRDIGFWCPRHLKTSGGQGYKFLGIDQCAPPCPNMYFKS
DELEFAKSFIGTVSIFCLCATLFTFLTFLIDVRRFRYPERPIIYYSVCYSIVSLMYFI
GFLLGDSTACNKADEKLELGDTVVLGSQNKACTVLFMLLYFFTMAGTVWWVILTITWF
LAAGRKWSCEAIEQKAVWFHAVAWGTPGFLTVMLLAMNKVEGDNISGVCFVGLYDLDA
SRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRNQEKLKKFMIRIGVFSGLY
LVPLVTLLGCYVYEQVNRITWEITWVSDHCRQYHIPCPYQAKAKARPELALFMIKYLM
TLIVGISAVFWVGSKKTCTEWAGFFKRNRKRDPISESRRVLQESCEFFLKHNSKVKHK
KKHYKPSSHKLKVISKSMGTSTGATANHGTSAVAITSHDYLGQETLTEIQTSPETSMR
EVKADGASTPRLREQDCGEPASPAASISRLSGEQVDGKGQAGSVSESARSEGRISPKS
DITDTGLAQSNNLQVPSSSEPSSLKGSTSLLVHPVSGVRKEQGGGCHSDT"
BASE COUNT 761 a 521 c 597 g 770 t
ORIGIN
1 tctcgctccc cgtgcggctc tcgaggcagc tccagtcccg gacgcaaccc cggagccgtc
61 tcaggtccct ggggggaacg gtgggttaga cggggacggg aagggacagc ggccttcgac
121 cgccccccga gtaattgacc caggactcat tttcaggaaa gcctgaaaat gagtaaaata
181 gtgaaatgag gaatttgaac attttatctt tggatgggga tcttctgagg atgcaaagag
241 tgattcatcc aagccatgtg atgtatgaaa atggcctaca acatgacgtt tttccctaat
301 ctgatgggtc attatgacca gagtattgcc gcggtggaaa tggagcattt tcttcctctc
361 gcaaatctgg aatgttcacc aaacattgaa actttcctct gcaaagcatt tgtaccaacc
421 tgcatagaac aaattcatgt ggttccacct tgtcgtaaac tttgtgagaa agtatattct
481 gattgcaaaa aattaattga cacttttggg atccgatggc ctgaggagct tgaatgtgac
541 agattacaat actgtgatga gactgttcct gtaacttttg atccacacac agaatttctt
601 ggtcctcaga agaaaacaga acaagtccaa agagacattg gattttggtg tccaaggcat
661 cttaagactt ctgggggaca aggatataag tttctgggaa ttgaccagtg tgcgcctcca
721 tgccccaaca tgtattttaa aagtgatgag ctagagtttg caaaaagttt tattggaaca
781 gtttcaatat tttgtctttg tgcaactctg ttcacattcc ttactttttt aattgatgtt
841 agaagattca gatacccaga gagaccaatt atatattact ctgtctgtta cagcattgta
901 tctcttatgt acttcattgg atttttgcta ggcgatagca cagcctgcaa taaggcagat
961 gagaagctag aacttggtga cactgttgtc ctaggctctc aaaataaggc ttgcaccgtt
1021 ttgttcatgc ttttgtattt tttcacaatg gctggcactg tgtggtgggt gattcttacc
1081 attacttggt tcttagctgc aggaagaaaa tggagttgtg aagccatcga gcaaaaagca
1141 gtgtggtttc atgctgttgc atggggaaca cctggtttcc tgactgttat gcttcttgct
1201 atgaacaaag ttgaaggaga caacattagt ggagtttgct ttgttggcct ttatgacctg
1261 gatgcttctc gctactttgt actcttgcca ctgtgccttt gtgtgtttgt tgggctctct
1321 cttcttttag ctggcattat ttccttaaat catgttcgac aagtcataca acatgatggc
1381 cggaaccaag aaaaactaaa gaaatttatg attcgaattg gagtcttcag cggcttgtat
1441 cttgtgccat tagtgacact tctcggatgt tacgtctatg agcaagtgaa caggattacc
1501 tgggagataa cttgggtctc tgatcattgt cgtcagtacc atatcccatg tccttatcag
1561 gcaaaagcaa aagctcgacc agaattggct ttatttatga taaaatacct gatgacatta
1621 attgttggca tctctgctgt cttctgggtt ggaagcaaaa agacatgcac agaatgggct
1681 gggtttttta aacgaaatcg caagagagat ccaatcagtg aaagtcgaag agtactacag
1741 gaatcatgtg agtttttctt aaagcacaat tctaaagtta aacacaaaaa gaagcactat
1801 aaaccaagtt cacacaagct gaaggtcatt tccaaatcca tgggaaccag cacaggagct
1861 acagcaaatc atggcacttc tgcagtagca attactagcc atgattacct aggacaagaa
1921 actttgacag aaatccaaac ctcaccagaa acatcaatga gagaggtgaa agcggacgga
1981 gctagcaccc ccaggttaag agaacaggac tgtggtgaac ctgcctcgcc agcagcatcc
2041 atctccagac tctctgggga acaggtcgac gggaagggcc aggcaggcag tgtatctgaa
2101 agtgcgcgga gtgaaggaag gattagtcca aagagtgata ttactgacac tggcctggca
2161 cagagcaaca atttgcaggt ccccagttct tcagaaccaa gcagcctcaa aggttccaca
2221 tctctgcttg ttcacccggt ttcaggagtg agaaaagagc agggaggtgg ttgtcattca
2281 gatacttgaa gaacattttc tctcgttact cagaagcaaa tttgtgttac actggaagtg
2341 acctatgcac tgttttgtaa gaatcactgt tacattcttc ttttgcactt aaagttgcat
2401 tgcctactgt tatactggaa aaaatagagt tcaagaataa tatgactcat ttcacacaaa
2461 ggttaatgac aacaatatac ctgaaaacag aaatgtgcag gttaataata tttttttaat
2521 agtgtgggag gacagagtta gaggaatctt ccttttctat ttatgaagat tctactcttg
2581 gtaagagtat tttaagatgt actatgctat tttacttttt tgatataaaa tcaagatatt
2641 tctttgctg
//