LOCUS AK299338 2942 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60069 complete cds, highly similar to Discs
large homolog 7.
ACCESSION AK299338
VERSION AK299338.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2942)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2942
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8005978"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493962"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 95..2623
/codon_start=1
/note="highly similar to Discs large homolog 7"
/protein_id="BAG61340.1"
/transl_table=1
/translation="MSSSHFASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRH
FGLKDVNIPTLEGRILVELDETSQGLVPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQ
LQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKAEPKKAIPSSVRITRSKAKDQ
MEQTKIDNESDVRAIRPGPRQTSEKKVSDKEKKVVQPVMPTSLRMTRSATQAAKQVPR
TVSSTTARKPVTRAANENEPEGKVPSKGRPAKNVETKPDKGISCKVDSEENTLNSQTN
ATSGMNPDGVLSKMENLPEINTAKIKGKNSFAPKDFMFQPLDGLKTYQVTPTTPRSAN
AFLTPSYTWTPLKTEVDESQATKEILAQKCKTYSTKTIQQDSNKLPCPLGPLTVWHEE
HVLNKNEATTKNLNGLPIKEVPSLERNEGRIAQPHHGVPYFRNILQSETEKLTSHCFE
WDRKLELDIPDDAKDLIRTAVGQTRLLMKERFKQFEGLVDDCEYKRGIKETTCTDLDG
FWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIASKPKQDD
AGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVKLFS
GLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLFL
SIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTN
KKEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILEEGETKISQSELFDNKSLT
TECHLLDSVGSCYVARAGLEVLGSSDPTTSASRVAGTTARSKLQ"
BASE COUNT 1038 a 514 c 612 g 778 t
ORIGIN
1 gagggttcct gcttcggagt cggcggtggt cgtccagacc gagtgttctt tactttttgt
61 ttggttgagg tttcacgcta gaaggtggct caggatgtct tcatcacatt ttgccagtcg
121 acacaggaag gatataagta ctgaaatgat tagaactaaa attgctcata ggaaatcact
181 gtctcagaaa gaaaatagac ataaggaata cgaacgaaat agacactttg gtttgaaaga
241 tgtaaacatt ccaaccttgg aaggtagaat tcttgttgaa ttagatgaga catctcaagg
301 gcttgttcca gaaaagacca atgttaagcc aagggcaatg aaaactattc taggtgatca
361 acgaaaacag atgctccaaa aatacaaaga agaaaagcaa cttcaaaaat tgaaagagca
421 gagagagaaa gctaaacgag gaatatttaa agtgggtcgt tatagacctg atatgccttg
481 ttttctttta tcaaaccaga atgctgtgaa agctgagcca aaaaaggcta ttccatcttc
541 tgtacggatt acaaggtcaa aggccaaaga ccaaatggag cagactaaga ttgataacga
601 gagtgatgtt cgagcaatcc gacctggtcc aagacaaact tctgaaaaga aagtgtcaga
661 caaagagaaa aaagttgtgc agcctgtaat gcccacgtcg ttgagaatga ctcgatcagc
721 tactcaagca gcaaagcagg ttcccagaac agtctcatct accacagcaa gaaagccagt
781 cacaagagct gctaatgaaa acgaaccaga aggaaaggtg ccaagtaaag gaagacctgc
841 caaaaatgta gaaacaaaac ccgacaaggg tatttcttgt aaagtcgata gtgaagaaaa
901 tactttgaat tcacaaacta atgcaacaag tggaatgaat ccagatggag tcttatcaaa
961 aatggaaaac ttacctgaga taaatactgc aaaaataaaa gggaagaatt cctttgcacc
1021 taaggatttt atgtttcagc cactggatgg tctgaagacc tatcaagtaa cacctacgac
1081 tcccagaagt gccaatgctt ttttgacacc cagttacacc tggactcctt taaaaacaga
1141 agttgatgag tctcaagcaa caaaagaaat tttggcacaa aaatgtaaaa cttactctac
1201 caagacaata cagcaagatt caaataaatt gccatgtcct ttgggtcctc taactgtttg
1261 gcatgaagaa catgttttaa ataaaaatga agctactact aaaaatttaa atggccttcc
1321 aataaaagaa gtcccatcac ttgaaagaaa tgaaggtcga attgctcagc cccaccatgg
1381 tgtgccatat ttcagaaata tcctccagtc agaaactgag aaattaactt cacattgctt
1441 cgagtgggac aggaaacttg aattggacat tccagatgat gctaaagatc ttattcgcac
1501 agcagttggt caaacaagac tccttatgaa ggaaaggttt aaacagtttg aaggactggt
1561 tgatgattgt gaatataaac gaggtataaa ggagactacc tgtacagatc tggatggatt
1621 ttgggatatg gttagttttc agatagaaga tgtaatccac aaattcaaca atctgatcaa
1681 acttgaggaa tctgggtggc aagtcaataa taatatgaat cataatatga acaaaaatgt
1741 ctttaggaaa aaagttgtct caggtatagc aagtaaacca aaacaggatg atgctggaag
1801 aattgcagcg agaaatcgcc tagctgccat aaaaaatgca atgagagaga gaattaggca
1861 ggaagaatgt gctgaaacag cagtttctgt gataccaaag gaagttgata aaatagtgtt
1921 cgatgctgga tttttcagag ttgaaagtcc tgttaaatta ttctcaggac tttctgtctc
1981 ttctgaaggc ccttctcaaa gacttggaac acctaagtct gtcaacaaag ctgtatctca
2041 gagtagaaat gagatgggca ttccacaaca aactacatca ccagaaaatg ccggtcctca
2101 gaatacgaaa agtgaacatg tgaagaagac tttgtttttg agtattcctg aaagcaggag
2161 cagcatagaa gatgctcagt gtcctggatt accagattta attgaagaaa atcatgttgt
2221 aaataagaca gacttgaagg tggattgttt atccagtgag agaatgagtt tgcctcttct
2281 tgctggtgga gtagcagatg atattaatac taacaaaaaa gaaggaattt cagatgttgt
2341 ggaaggaatg gaactgaatt cttcaattac atcacaggat gttttgatga gtagccctga
2401 aaaaaataca gcttcacaaa atagcatctt agaagaaggg gaaactaaaa tttctcagtc
2461 agaactattt gataataaaa gtctcactac tgaatgccac cttcttgatt cagtgggatc
2521 ttgctatgtt gctcgggctg gtcttgaagt cctgggttca agtgatccta ccacctcagc
2581 ctcccgagta gctgggacta cagccaggtc taaactgcag taatccattt actcagctgg
2641 agaggagaca tcaagaacat gccagacaca tttcttttgg tggtaacctg attacttttt
2701 cacctctaca accaggagaa ttttgaattt aaaaataaat ccaaacattt tccttcatat
2761 tatcaatgct tatatattcc ttagactatt gaaattttgg agaaaatgta tttgtgttca
2821 cttctatagc atataatgtt ttaatattct gtgttcatca aagtgtattt tagatatact
2881 ctttctcaag ggaagtgggg atattttgta cattttcaac acagaataaa aaatgtactg
2941 tg
//