LOCUS AK299335 2335 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50843 complete cds, highly similar to
Neuroepithelial cell-transforming gene 1 protein.
ACCESSION AK299335
VERSION AK299335.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2335)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2335
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8005626"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493959"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 99..1889
/codon_start=1
/note="highly similar to Neuroepithelial
cell-transforming gene 1 protein"
/protein_id="BAG61338.1"
/transl_table=1
/translation="MEPELAAQKQPRPRRRSRRASGLSTEGATGPSADTSGSELDGRC
SLRRGSSFTFLTPGPNWDFTLKRKRREKDDDVVSLSSLDLKEPSNKRVRPLARVTSLA
NLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEML
DITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHI
FGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKAL
LDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHP
DVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGE
LRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGG
SFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGS
PPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDS
KSLKTHQTQPGIRRARDKALSGGKRKETLV"
BASE COUNT 633 a 560 c 566 g 576 t
ORIGIN
1 gacaggcgct ttctgcctgg cagaggctgg cgggcatcgt gcccgtccct gccggtctcc
61 cgggcacccg gccaccgccc caccccctcc tccgtgccat ggagcccgag ctggcggctc
121 agaagcagcc tcgaccgcgg aggcgaagcc gccgggcctc tgggctcagc acggagggag
181 cgacggggcc ttcggccgac acctccgggt cggagctgga cgggagatgt tcccttcgga
241 gaggcagctc cttcacattc ttaacacctg gccccaactg ggacttcact ttgaaaagaa
301 aacgcagaga gaaagatgat gatgttgtaa gccttagcag ccttgatctg aaggagccaa
361 gcaataaaag agttcgacct ctggctcgtg tcacgtcctt ggcaaattta atctctcctg
421 taagaaatgg agctgtcaga cgttttggtc aaacaataca gtcatttacc cttcgtggtg
481 accacagatc cccagcctct gcccagaagt tttctagcag gtcaacagtc ccaacacccg
541 ccaagagaag gagcagtgca ctgtggtcag agatgctgga catcaccatg aaggagtctc
601 tcaccaccag ggagatcaga cggcaggagg caatatatga aatgtcccga ggtgaacagg
661 atttaattga ggatctcaaa cttgcaagaa aggcctacca tgaccccatg ttaaagttgt
721 ccatcatgtc agaagaggaa ctcacacata tatttggtga tctggactct tacatacctc
781 tgcatgaaga tttgttgaca agaataggag aagcaaccaa gcctgatgga acagtggagc
841 agattggtca cattctcgtg agctggttac cgcgcttgaa tgcctacaga ggttactgta
901 gtaaccagct ggcagccaaa gctcttcttg atcaaaagaa acaggatcca agagtccaag
961 acttcctcca gcgatgtctc gagtctccct tcagtcgaaa actagatctt tggagtttcc
1021 tagatatccc tcgaagtcgc ctagtcaaat accctttact gttaaaagaa attcttaaac
1081 acactccaaa agagcaccct gatgttcagc ttctggagga tgctatattg ataatacagg
1141 gagtcctctc tgacatcaac ttgaagaaag gtgaatccga gtgccagtat tacatcgaca
1201 agctggagta cctggatgaa aagcagaggg accccagaat cgaagcgagc aaagtgctgc
1261 tgtgccatgg ggagctgcgg agcaagagtg gacataaact ttacattttc ctgtttcaag
1321 acatcttggt tctgactcgg cccgtcacac ggaacgaacg gcactcttac caggtttacc
1381 ggcagccaat cccagtccaa gagctagtcc tagaagacct gcaggatgga gatgtgagaa
1441 tgggaggctc ctttcgagga gctttcagta actcagagaa agctaaaaat atctttagaa
1501 ttcgcttcca tgacccctct ccagcccagt ctcacactct gcaagccaat gacgtgttcc
1561 acaagcagca gtggttcaac tgtattcgag cggccattgc ccccttccag tcggcaggca
1621 gtccacctga gctgcagggc ctgccggagc tgcacgaaga gtgtgagggg aaccacccct
1681 ctgcgaggaa actcacagcc cagaggaggg catccacagt ttccagtgtt actcaggtag
1741 aagttgatga aaacgcttac agatgtggct ctggcatgca gatggcagag gacagcaaga
1801 gcttaaagac acaccagaca cagcccggca tccgaagagc gagggacaaa gccctttctg
1861 gtggcaaacg gaaagagact ttggtgtaga gaaggctctg tgtgttaact gatgggagag
1921 actgtttgtt tataaatgtg tacagttttg ttttctcgta aggggagcat catagggtta
1981 ctttatacca gttgtaacat tttcattgtt tttggttgtt cttttttctt tttttaatgg
2041 cagctaaaga tatacagatt actgttaaat tgcagtcctt ttttttttta aagatatttt
2101 cttgagttat ttagaacatg gtaagcctgg tattttttaa tcaaacaaaa tatttatgaa
2161 atgggttttc tcttaattct ggattcatca tggctttcta ataccaattg taatatttac
2221 aatattcacc aaaacttaga attttgcaaa tgctggaatt ctgccagtgt ttctttgcta
2281 agccttgcat gcaaaatttg aaattttaac attggcaccc aaaacctaca tggaa
//