LOCUS AK299318 2635 bp mRNA linear HUM 31-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60834 complete cds, highly similar to
Engulfment and cell motility protein 2 (CED-12 homolog A).
ACCESSION AK299318
VERSION AK299318.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2635)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2635
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8004715"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493942"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 205..2403
/codon_start=1
/note="highly similar to Engulfment and cell motility
protein 2 (CED-12 homolog A)"
/protein_id="BAG61327.1"
/transl_table=1
/translation="MPPPSDIVKVAIEWPGANAQLLEIDQKRPLASIIKEVCDGWSLP
NPEYYTLRYADGPQLYITEQTRSDIKNGTILQLAISPSRAARQLMERTQSSNMETRLD
AMKELAKLSADVTFATEFINMDGIIVLTRLVESGTKLLSHYSEMLAFTLTAFLELMDH
GIVSWDMVSITFIKQIAGYVSQPMVDVSILQRSLAILESMVLNSQSLYQKIAEEITVG
QLISHLQVSNQEIQTYAIALINALFLKAPEDKRQDKHLNPLDLPVTDMANAFAQKHLR
SIILNHVIRGNRPIKTEMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRI
AFDAESDPSNAPGSGTEKRKAMYTKDYKMLGFTNHINPAMDFTQTPPGMLALDNMLYL
AKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEILQVGELPNEGRNDYHPMF
FTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVREQITRALPSKPNSLDQFK
SKLRSLSYSEILRLRQSERMSQDDFQSPPIVELREKIQPEILELIKQQRLNRLCEGSS
FRKIGNRRRQERFWYCRLALNHKVLHYGDLDDNPQGEVTFESLQEKIPVADIKAIVTG
KDCPHMKEKSALKQNKEVLELAFSILYDPDETLNFIAPNKYEYCIWIDGLSALLGKDM
SSELTKSDLDTLLSMEMKLRLLDLENIQIPEAPPPIPKEPSSYDFVYHYG"
BASE COUNT 690 a 692 c 677 g 576 t
ORIGIN
1 gaaggcgggg cgggcggagg ctggaggagc cgcggggcgg agacccggga gcaggagctg
61 ggcctagatc tgcgccctgg agggaggtgt agaaagaggt acatggagaa caagtttgtc
121 aatccgtctg aacttcagtt gccttacctg taaggcagcc gtgtctgtgt ttttgtctcg
181 cagaattaga gcccattggg aacgatgcca ccaccgtcag acattgtcaa agtggccatt
241 gagtggccag gtgctaacgc ccagctcctt gaaatcgacc agaaacggcc cctggcatcc
301 attatcaagg aagtttgtga tgggtggtcg ttgccaaacc cagagtatta taccctccgt
361 tatgcagatg gtcctcagct gtacatcacc gaacagactc gcagtgacat taagaatggg
421 acaatcttac aactggctat ctccccgtcc cgggctgcac gccagctgat ggagaggacc
481 cagtcatcca acatggagac ccggctggat gccatgaagg agctggccaa gctctctgcc
541 gacgtgactt tcgctactga gttcatcaac atggatggca tcattgtgct gacaaggctc
601 gtggaaagtg gaaccaagct cttgtcccac tacagtgaga tgctggcatt caccctgact
661 gccttcctag agctcatgga ccatggcatt gtctcctggg acatggtttc aatcaccttt
721 attaagcaga ttgcagggta tgtgagccag cccatggtgg acgtgtcaat ccttcagagg
781 tccctggcca tcctggagag catggtcttg aacagccaga gtctgtacca gaagatagcc
841 gaggaaatca ccgtgggaca gctcatctca cacctccagg tctccaacca ggagattcag
901 acctacgcca ttgcactgat taatgcactt tttctgaagg ctcctgagga caaacgacag
961 gacaagcacc ttaatcctct agacctgcct gtcactgata tggcaaatgc atttgcacag
1021 aagcatctcc ggtctataat cctgaatcat gtgatccgag ggaaccgccc catcaaaact
1081 gagatggccc atcagctata tgtccttcaa gtcctaacct ttaaccttct ggaagaaagg
1141 atgatgacca agatggaccc caatgaccag gctcaaaggg acatcatatt tgaactgagg
1201 aggattgcat ttgacgcaga gtctgatcct agcaatgccc ctgggagtgg gaccgaaaaa
1261 cgcaaagcca tgtacacaaa ggactacaaa atgctgggat ttaccaacca catcaatcca
1321 gccatggact ttacccagac tcctcctgga atgctggcct tggacaacat gctgtacttg
1381 gctaaagtcc accaggacac ctacatccgg attgtcttgg agaacagtag ccgggaagac
1441 aaacatgaat gcccctttgg ccgcagtgcc attgagctca ccaaaatgct ctgtgaaatc
1501 ctgcaggttg gggaactacc aaatgaagga cgcaatgact accacccgat gttctttacc
1561 catgaccgag cctttgaaga gctctttgga atctgcatcc agctgttgaa caagacctgg
1621 aaggagatga gggcaacagc agaggacttc aacaaggtta tgcaagtcgt ccgagagcaa
1681 atcactcgag ctttgccctc caaacccaac tctttggatc agttcaagag caaattgcgt
1741 agcctgagtt actctgagat tctacgactg cgccagtctg agaggatgag tcaggatgac
1801 ttccagtccc cgccaattgt ggagctgagg gagaagatcc agcccgagat ccttgagctg
1861 atcaagcagc agcgcctgaa ccggctctgt gagggcagca gcttccgaaa gattgggaac
1921 cgccgaaggc aagaacggtt ctggtactgc cggttggcac tgaaccacaa ggtccttcac
1981 tatggtgact tggatgacaa cccacaaggg gaggtgacat ttgaatccct gcaggagaaa
2041 attcctgttg cagacattaa ggccattgtc actgggaaag attgtcccca catgaaagag
2101 aaaagtgctc tgaaacagaa caaggaggtg ttggaattgg ccttctccat cctgtatgac
2161 cctgatgaga ccttaaactt catcgcacct aataaatatg agtactgcat ctggattgac
2221 ggcctcagtg cccttctggg gaaggacatg tccagtgagc tgaccaagag tgacctggac
2281 accctgctga gcatggagat gaagctgcgg ctcctggacc tggagaacat ccagattccc
2341 gaagccccac cccccatccc caaggagccc agcagctatg actttgtcta tcactatggc
2401 tgagcctgga gccagaaacg acggtaccca ggagaaggga ttttgggccc aggagaaaca
2461 cttacattct ggtgccttgt cttttgcttg tacagaatct gtagtgattt tggtggccag
2521 taaatgccag ccatttctca aacccacctc ggaccaccca gagtttcctc ttggtccctg
2581 tctactaaga gtcatgaagg cagggtgctc tgcccactcc atcaccatga agcct
//