LOCUS AK299314 1588 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60138 complete cds.
ACCESSION AK299314
VERSION AK299314.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1588)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1588
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8004531"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493938"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 429..1220
/codon_start=1
/protein_id="BAG61324.1"
/transl_table=1
/translation="MGERAGSPGTDQERKAGKHHYSYSSDFETPQSSGRSSLVSSSPA
SVRRKNPKRQTSDGQVHHQAPRKPSPKGLPNRKGVRVGFRSQSLNREPLRKDTDLVTK
RILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQYRQEKALNKFEDAENEISQL
IFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEISEARHLPER
DDLAKKLVSAELKLDDTERRIKVQFLQLLIVLSWDVSHWAFNVLS"
BASE COUNT 509 a 314 c 349 g 416 t
ORIGIN
1 agcccaggtt accgccgctg ccacccagga gccccgatcc tcgcctctgt cccatccttg
61 tgttcaaacc tcccgcatct cggcaacctc ggcacccgcc cggcagcctc cgcaggaacc
121 aggcacccgc tctttggcgg tcagacgccg aggccccagc tgggagtttg gtcctaagag
181 ggaaggcaag gaggcgggac gccgcatcgg ctctgctgaa gagcctgcgg gttggagggc
241 aaaaaagttt atcttctgga tgccaatgtg aattgtggtc tacaaataca ttgtggagaa
301 aatagattgc acagaaatga atattatcag gatctgaaga ctgtgaaaat gtttttcagt
361 attgtcgtag tctcctctgg agaaaataat ctgtgaaatt atgtgaatag agaccatttt
421 tcaaaacaat gggggaaaga gcaggaagtc caggtactga tcaagaaaga aaggcaggca
481 aacaccatta ttcttactca tctgattttg aaacgccaca gtcttctggc cgatcatcgc
541 tggtcagttc ttcacctgca agtgttagga gaaaaaatcc taaaagacaa acttcagatg
601 gccaagtaca tcaccaagcc cctcggaaac caagccctaa gggtctacca aacagaaagg
661 gagtccgagt gggatttcgc tcccagagcc tcaatagaga gccacttcgg aaagatactg
721 atcttgttac aaaacggatt ctgtctgcaa gactgctaaa aatcaatgag ttgcagaatg
781 aagtatctga actccaggtc aagttagctg agctgctaaa agaaaataaa tctttgaaaa
841 ggcttcagta cagacaggag aaagccctga ataagtttga agatgccgaa aatgaaatct
901 cacaacttat atttcgtcat aacaatgaga ttacagcact caaagaacgc ttaagaaaat
961 ctcaagagaa agaacgggca actgagaaaa gggtaaaaga tacagaaagt gaactattta
1021 ggacaaaatt ttccttacag aaactgaaag agatctctga agctagacac ctacctgaac
1081 gagatgattt ggcaaagaaa ctagtttcag cagagttaaa gttagatgac accgagagaa
1141 gaattaaggt acaatttcta cagcttctaa ttgtactttc ttgggatgtt tctcattggg
1201 catttaatgt gctctcttaa aattgcttat tgcagtttgg tatttgcttg gaaatgaaaa
1261 ctatttttag tactgttttg ggaaaatttg gattttatat ttaagaggaa gatgcaaaag
1321 attgtgtaca ttaatgagct acatgttgat tgacggatca tagtcatttt gggaaaatac
1381 tctatttggt tatctgattg tttctctaaa attggaaatg acactaatag ccatagtata
1441 taaaactgac gttttggggc cactgtgtaa gagatgccac atgaacaatg acttctgttt
1501 ttatatttat gcacattatt tatcgaagat taattacagt acatcttttt ggacatggaa
1561 tgcacctcct cccccgaatg aaaaatct
//