LOCUS AK299306 4491 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60402 complete cds, highly similar to
Pro-epidermal growth factor precursor.
ACCESSION AK299306
VERSION AK299306.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4491)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..4491
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8003939"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000493930"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 455..3952
/codon_start=1
/note="highly similar to Pro-epidermal growth factor
precursor"
/protein_id="BAG61319.1"
/transl_table=1
/translation="MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGP
APFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVF
LNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYP
ANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQ
YNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHT
GKDMVRINLHSSFVPLGELKVVHPLTQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPG
SYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGK
TCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQD
IRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLI
EEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPM
AKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVI
EMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVIRVNKRTGKDRVRLQGS
MLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLA
LDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGC
SMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCR
CSEGYQGDGIHCLDIDECQLGVHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTP
PPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQY
RDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYE
ESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTS
WRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSA
NPLWQQRALDPPHQMELTQ"
BASE COUNT 1243 a 908 c 1116 g 1224 t
ORIGIN
1 aaaaaaagag aaactgttgg gagaggaatc gtatctccat atttcttctt tcagccccaa
61 tccaagggtt gtggctggaa ctttccatca gttcttcctt tctttttcct ctctaagcct
121 ttgccttgct ctgtcacagt gaagtcagcc agagcagggc tgttaaactc tgtgaaattt
181 gtcataaggg tgtcaggtat ttcttactgg cttccaaaga aacatagata aagaaatctt
241 tcctgtggct tcccttggca ggctgcattc agaaggtctc tcagttgaag aaagagcttg
301 gaggacaaca gcacaacagg agagtaaaag atgccccagg gctgaggcct ccgctcaggc
361 agccgcatct ggggtcaatc atactcacct tgcccgggcc atgctccagc aaaatcaagc
421 tgttttcttt tgaaagttca aactcatcaa gattatgctg ctcactctta tcattctgtt
481 gccagtagtt tcaaaattta gttttgttag tctctcagca ccgcagcact ggagctgtcc
541 tgaaggtact ctcgcaggaa atgggaattc tacttgtgtg ggtcctgcac ccttcttaat
601 tttctcccat ggaaatagta tctttaggat tgacacagaa ggaaccaatt atgagcaatt
661 ggtggtggat gctggtgtct cagtgatcat ggattttcat tataatgaga aaagaatcta
721 ttgggtggat ttagaaagac aacttttgca aagagttttt ctgaatgggt caaggcaaga
781 gagagtatgt aatatagaga aaaatgtttc tggaatggca ataaattgga taaatgaaga
841 agttatttgg tcaaatcaac aggaaggaat cattacagta acagatatga aaggaaataa
901 ttcccacatt cttttaagtg ctttaaaata tcctgcaaat gtagcagttg atccagtaga
961 aaggtttata ttttggtctt cagaggtggc tggaagcctt tatagagcag atctcgatgg
1021 tgtgggagtg aaggctctgt tggagacatc agagaaaata acagctgtgt cattggatgt
1081 gcttgataag cggctgtttt ggattcagta caacagagaa ggaagcaatt ctcttatttg
1141 ctcctgtgat tatgatggag gttctgtcca cattagtaaa catccaacac agcataattt
1201 gtttgcaatg tccctttttg gtgaccgtat cttctattca acatggaaaa tgaagacaat
1261 ttggatagcc aacaaacaca ctggaaagga catggttaga attaacctcc attcatcatt
1321 tgtaccactt ggtgaactga aagtagtgca tccacttaca caacccaagg cagaagatga
1381 cacttgggag cctgatgtta atgaatgtgc tttttggaat catggctgta ctcttgggtg
1441 taaaaacacc cctggatcct attactgcac gtgccctgta ggatttgttc tgcttcctga
1501 tgggaaacga tgtcatcaac ttgtttcctg tccacgcaat gtgtctgaat gcagccatga
1561 ctgtgttctg acatcagaag gtcccttatg tttctgtcct gaaggctcag tgcttgagag
1621 agatgggaaa acatgtagcg gttgttcctc acccgataat ggtggatgta gccagctctg
1681 cgttcctctt agcccagtat cctgggaatg tgattgcttt cctgggtatg acctacaact
1741 ggatgaaaaa agctgtgcag cttcaggacc acaaccattt ttgctgtttg ccaattctca
1801 agatattcga cacatgcatt ttgatggaac agactatgga actctgctca gccagcagat
1861 gggaatggtt tatgccctag atcatgaccc tgtggaaaat aagatatact ttgcccatac
1921 agccctgaag tggatagaga gagctaatat ggatggttcc cagcgagaaa ggcttattga
1981 ggaaggagta gatgtgccag aaggtcttgc tgtggactgg attggccgta gattctattg
2041 gacagacaga gggaaatctc tgattggaag gagtgattta aatgggaaac gttccaaaat
2101 aatcactaag gagaacatct ctcaaccacg aggaattgct gttcatccaa tggccaagag
2161 attattctgg actgatacag ggattaatcc acgaattgaa agttcttccc tccaaggcct
2221 tggccgtctg gttatagcca gctctgatct aatctggccc agtggaataa cgattgactt
2281 cttaactgac aagttgtact ggtgcgatgc caagcagtct gtgattgaaa tggccaatct
2341 ggatggttca aaacgccgaa gacttaccca gaatgatgta ggtcacccat ttgctgtagc
2401 agtgtttgag gattatgtgt ggttctcaga ttgggctatg ccatcagtaa taagagtaaa
2461 caagaggact ggcaaagata gagtacgtct ccaaggcagc atgctgaagc cctcatcact
2521 ggttgtggtt catccattgg caaaaccagg agcagatccc tgcttatatc aaaacggagg
2581 ctgtgaacat atttgcaaaa agaggcttgg aactgcttgg tgttcgtgtc gtgaaggttt
2641 tatgaaagcc tcagatggga aaacgtgtct ggctctggat ggtcatcagc tgttggcagg
2701 tggtgaagtt gatctaaaga accaagtaac accattggac atcttgtcca agactagagt
2761 gtcagaagat aacattacag aatctcaaca catgctagtg gctgaaatca tggtgtcaga
2821 tcaagatgac tgtgctcctg tgggatgcag catgtatgct cggtgtattt cagagggaga
2881 ggatgccaca tgtcagtgtt tgaaaggatt tgctggggat ggaaaactat gttctgatat
2941 agatgaatgt gagatgggtg tcccagtgtg cccccctgcc tcctccaagt gcatcaacac
3001 cgaaggtggt tatgtctgcc ggtgctcaga aggctaccaa ggagatggga ttcactgtct
3061 tgatattgat gagtgccaac tgggggtgca cagctgtgga gagaatgcca gctgcacaaa
3121 tacagaggga ggctatacct gcatgtgtgc tggacgcctg tctgaaccag gactgatttg
3181 ccctgactct actccacccc ctcacctcag ggaagatgac caccactatt ccgtaagaaa
3241 tagtgactct gaatgtcccc tgtcccacga tgggtactgc ctccatgatg gtgtgtgcat
3301 gtatattgaa gcattggaca agtatgcatg caactgtgtt gttggctaca tcggggagcg
3361 atgtcagtac cgagacctga agtggtggga actgcgccac gctggccacg ggcagcagca
3421 gaaggtcatc gtggtggctg tctgcgtggt ggtgcttgtc atgctgctcc tcctgagcct
3481 gtggggggcc cactactaca ggactcagaa gctgctatcg aaaaacccaa agaatcctta
3541 tgaggagtcg agcagagatg tgaggagtcg caggcctgct gacactgagg atgggatgtc
3601 ctcttgccct caaccttggt ttgtggttat aaaagaacac caagacctca agaatggggg
3661 tcaaccagtg gctggtgagg atggccaggc agcagatggg tcaatgcaac caacttcatg
3721 gaggcaggag ccccagttat gtggaatggg cacagagcaa ggctgctgga ttccagtatc
3781 cagtgataag ggctcctgtc cccaggtaat ggagcgaagc tttcatatgc cctcctatgg
3841 gacacagacc cttgaagggg gtgtcgagaa gccccattct ctcctatcag ctaacccatt
3901 atggcaacaa agggccctgg acccaccaca ccaaatggag ctgactcagt gaaaactgga
3961 attaaaagga aagtcaagaa gaatgaacta tgtcgatgca cagtatcttt tctttcaaaa
4021 gtagagcaaa actataggtt ttggttccac aatctctacg actaatcacc tactcaatgc
4081 ctggagacag atacgtagtt gtgcttttgt ttgctctttt aagcagtctc actgcagtct
4141 tatttccaag taagagtact gggagaatca ctaggtaact tattagaaac ccaaattggg
4201 acaacagtgc tttgtaaatt gtgttgtctt cagcagtcaa tacaaataga tttttgtttt
4261 tgttgttcct gcagccccag aagaaattag gggttaaagc agacagtcac actggtttgg
4321 tcagttacaa agtaatttct ttgatctgga cagaacattt atatcagttt catgaaatga
4381 ttggaatatt acaataccgt taagatacag tgtaggcatt taactcctca ttggcgtggt
4441 ccatgctgat gattttgcaa aatgagttgt gatgaatcaa tgaaaaatgt a
//