LOCUS AK299254 1199 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56929 complete cds, highly similar to Antigen
KI-67.
ACCESSION AK299254
VERSION AK299254.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1199)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1199
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7016932"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000493878"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 6..569
/codon_start=1
/note="highly similar to Antigen KI-67"
/protein_id="BAG61285.1"
/transl_table=1
/translation="MKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAE
RIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVSENKRCLRSARQNESSQPKVAE
ESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLEGESVQRVTRSVKRC
AENPKKAEDNVCVKKIRTRSHRDSEDI"
BASE COUNT 385 a 242 c 311 g 261 t
ORIGIN
1 acgacatgaa aaccaacaaa gaggaacaca aattacaaga ctcggtccct gaaaataagg
61 gaatatccct gcgctccaga cgccaaaata agactgaggc agaacagcaa ataactgagg
121 tctttgtatt agcagaaaga atagaaataa acagaaatga aaagaagccc atgaagacct
181 ccccagagat ggacattcag aatccagatg atggagcccg gaaacccata cctagagaca
241 aagtcagtga gaacaaaagg tgcttgaggt ctgctagaca gaatgagagc tcccagccta
301 aggtggcaga ggagagcgga gggcagaaga gtgcgaaggt tctcatgcag aatcagaaag
361 ggaaaggaga agcaggaaat tcagactcca tgtgcctgag atcaagaaag acaaaaagcc
421 agcctgcagc aagcactttg gagggcgaat ctgtgcagag agtaacgcgg agtgtcaaga
481 gatgtgcaga aaatccaaag aaggctgagg acaatgtgtg tgtcaagaaa ataagaacca
541 gaagtcatag ggacagtgaa gatatttgac agaaaaatcg aactgggaaa aatataataa
601 agttagtttt gtgataagtt ctagtgcagt ttttgtcata aattacaagt gaattctgta
661 agtaaggctg tcagtctgct taagggaaga aaactttgga tttgctggct ctgaatcggc
721 ttcataaact ccactgggag cactgctggg ctcctggact gagaatagtt gaacaccggg
781 ggctttgtga aggagtctgg gccaaggttt gccctcagct ttgcagaatg aagccttgag
841 gtctgtcacc acccacagcc accctacagc agccttaact gtgacacttg ccacactgtg
901 tcgtcgtttg tttgcctatg tcctccaggg cacggtggcg ggaacaacta tcctcgtctg
961 tcccaacact gagcaggcac tcggtaaaca cgaatgaatg gatgagcgca cggatgaatg
1021 gagcttacaa gatctgtctt tccaatggcc gggggcattt ggtccccaaa ttaaggctat
1081 tggacatctg cacaggacag tcctattttt gatgtccttt cctttctgaa aataaagttt
1141 tgtgctttgg agaatgactc gtgagcacat ctttagggac caagagtgac tttctgtaa
//