LOCUS AK299232 2053 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58124 complete cds, highly similar to
Complement factor I precursor (EC 3.4.21.45).
ACCESSION AK299232
VERSION AK299232.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2053)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2053
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7013285"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000493856"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 81..1856
/codon_start=1
/note="highly similar to Complement factor I precursor
(EC 3.4.21.45)"
/protein_id="BAG61264.1"
/transl_table=1
/translation="MKLLHVFLLFLCFHLRFCKVTYTSQEDLVEKKCLAKKYTHLSCD
KVFCQPWQRCIEGTCVCKLPYQCPKNGTAVCATNRRSFPTYCQQKSLECLHPGTKFLN
NGTCTAEGKFSVSLKHGNTDSEEIVEVKLVDQDKTMFICKSSWSMREANVACLDLGFQ
QGADTQRRFKLSDLSINSTECLHVHCRGLETSLAECTFTKRRTMGYQDFADVVCYTQK
ADSPMDDFFQCVNGKYISQMKACDGINDCGDQSDELCCKACQGRGFHCKSGVCIPSQY
QCNGEVDCITGEDEVGCAAARHPTIQGFASVAQEETEILTADMDAERRRIKSLLPKLS
CGVKNRMHIRRKRIVGGKRAQLGDLPWQVAIKDASGITCGGIYIGGCWILTAAHCLRA
SKTHRYQIWTTVVDWIHPDLKRIVIEYVDRIIFHENYNAGTYQNDIALIEMKKDGNKK
DCELPRSIPACVPWSPYLFQPNDTCIVSGWGREKDNERVFSLQWGEVKLISNCSKFYG
NRFYEKEMECAGTYDGSIDACKGDSGGPLVCMDANNVTYVWGVVSWGENCGKPEFPGV
YTKVANYFDWISYHVGRPFISQYNV"
BASE COUNT 644 a 370 c 471 g 568 t
ORIGIN
1 ggttaaaaat taaatttcaa aagaatacct ggagtggaaa agagttctca gcagagacaa
61 agaccccgaa cacctccaac atgaagcttc ttcatgtttt cctgttattt ctgtgcttcc
121 acttaaggtt ttgcaaggtc acttatacat ctcaagagga tctggtggag aaaaagtgct
181 tagcaaaaaa atatactcac ctctcctgcg ataaagtctt ctgccagcca tggcagagat
241 gcattgaggg cacctgtgtt tgtaaactac cgtatcagtg cccaaagaat ggcactgcag
301 tgtgtgcaac taacaggaga agcttcccaa catactgtca acaaaagagt ttggaatgtc
361 ttcatccagg gacaaagttt ttaaataacg gaacatgcac agccgaagga aagtttagtg
421 tttccttgaa gcatggaaat acagattcag aggaaatagt tgaagtaaaa cttgtggacc
481 aagataagac aatgttcata tgcaaaagca gctggagcat gagggaagcc aacgtggcct
541 gccttgacct tgggtttcaa caaggtgctg atactcaaag aaggtttaag ttgtctgatc
601 tctctataaa ttccactgaa tgtctacatg tgcattgccg aggattagag accagtttgg
661 ctgaatgtac ttttactaag agaagaacta tgggttacca ggatttcgct gatgtggttt
721 gttatacaca gaaagcagat tctccaatgg atgacttctt tcagtgtgtg aatgggaaat
781 acatttctca gatgaaagcc tgtgatggta tcaatgattg tggagaccaa agtgatgaac
841 tgtgttgtaa agcatgccaa ggcagaggct tccattgcaa atcaggtgtt tgcattccaa
901 gccagtatca atgcaatggt gaggtggact gcattacagg ggaagatgaa gttggctgtg
961 cagcagctag acatcctaca attcaaggct ttgcatctgt ggctcaagaa gaaacagaaa
1021 ttttgactgc tgacatggat gcagaaagaa gacggataaa atcattatta cctaaactat
1081 cttgtggagt taaaaacaga atgcacattc gaaggaaacg aattgtggga ggaaagcgag
1141 cacaactggg agacctccca tggcaggtgg caattaagga tgccagtgga atcacctgtg
1201 ggggaattta tattggtggc tgttggattc tgactgctgc acattgtctc agagccagta
1261 aaactcatcg ttaccaaata tggacaacag tagtagactg gatacacccc gaccttaaac
1321 gtatagtaat tgaatacgtg gatagaatta ttttccatga aaactacaat gcaggcactt
1381 accaaaatga catcgctttg attgaaatga aaaaagacgg aaacaaaaaa gattgtgagc
1441 tgcctcgttc catccctgcc tgtgtcccct ggtctcctta cctattccaa cctaatgata
1501 catgcatcgt ttctggctgg ggacgagaaa aagataacga aagagtcttt tcacttcagt
1561 ggggtgaagt taaactaata agcaactgct ctaagtttta cggaaatcgt ttctatgaaa
1621 aagaaatgga atgtgcaggt acatatgatg gttccatcga tgcctgtaaa ggggactctg
1681 gaggcccctt agtctgtatg gatgccaaca atgtgactta tgtctggggt gttgtgagtt
1741 ggggggaaaa ctgtggaaaa ccagagttcc caggtgttta caccaaagtg gccaattatt
1801 ttgactggat tagctaccat gtaggaaggc cttttatttc tcagtacaat gtataaaatt
1861 gtgatctctc tcttcattct attctttttc tctcaagagt tccatttaat ggaaataaaa
1921 cggtataatt aataattctc taggggggaa aaatgaagca aatctcattg gatattttta
1981 aaggtctcca cagagtttat gccatattgg aattttgttg tataattctc aaataaatat
2041 tttggtgaag cat
//