LOCUS AK299203 2274 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59742 complete cds, highly similar to mRNA
decapping enzyme 1B (EC 3.-.-.-).
ACCESSION AK299203
VERSION AK299203.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2274)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2274
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7007788"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000493827"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 472..2019
/codon_start=1
/note="highly similar to mRNA decapping enzyme 1B (EC
3.-.-.-)"
/protein_id="BAG61243.1"
/transl_table=1
/translation="MQIKVSIYGIWFYDKEECQRIAELMKNLTQYEQLKAHQGTGAGI
SPVILNSGEGKEVDILRMLIKAKDEYTKCKTCSEPKKITSSSAIYDNPNLIKPIPVKP
SENQQQRIPQPNQTLDPEPQHLSLTALFGKQDKATCQETVEPPQTLHQQQQQQQQQQE
KLPIRQGVVRSLSYEEPRRHSPPIEKQLCPAIQKLMVRSADLHPLSELPENRPCENGS
THSAGEFFTGPVQPGSPHNIGTSRGVQNASRTQNLFEKLQSTPGAANKCDPSTPAPAS
SAALNRSRAPTSVTPVAPGKGLAQPPQAYFNGSLPPQTVGHQAHGREQSTLPRQTLPI
SGSQTGSSGVISPQELLKKLQIVQQEQQLHASNRPALAAKFPVLAQSSGTGKPLESWI
NKTPNTEQQTPLFQVISPQRIPATAAPSLLMSPMVFAQPTSVPPKERESGLLPVGGQE
PPAAATSLLLPIQSPEPSVITSSPLTKLQLQEALLYLIQNDDNFLNIIYEAYLFSMTQ
AAMKKTM"
BASE COUNT 663 a 648 c 510 g 453 t
ORIGIN
1 ttccaagagc cccgtgccta tgtctgggag ggagggaaga tggcagccgt ggcggcaggc
61 ggcctggtgg gaaaggggcg cgacatcagc ctagcggccc tgcagcgcca cgacccctat
121 atcaaccgca tcgtggacgt ggccagccag gtggctctgt acaccttcgg ccatcgggcc
181 aacgagtggg agaaaactga tgtggaagga accttatttg tttatacaag gtctgcttct
241 ccaaagcatg gattcaccat tatgaatagg ctgagcatgg aaaataggac agaacctatt
301 actaaagact tggatttcca actccaggac cctttccttc tctacagaaa tgccagatat
361 gaggaaactg agactccagt gaggaggcag actgtgtaaa aacccacaga ttctgatttc
421 acttccgttg ctttttccac accaccggta ctgctctact attaaacatg gatgcaaatt
481 aaagtgtcca tctatggaat ttggttttat gataaggaag aatgccaaag aattgcagag
541 cttatgaaaa acctaactca gtatgaacag ttgaaagccc atcagggaac tggagcagga
601 atttccccag tgatcctcaa ttcaggagag ggcaaagaag tagacatttt acgaatgctc
661 atcaaggcca aagacgaata cacaaagtgt aaaacctgtt ctgagccaaa aaagataacc
721 agttcctctg ccatctatga caatccaaat ctcatcaaac caattccagt gaaacccagt
781 gaaaaccagc aacagcgtat acctcagccc aaccagacct tagaccctga accccaacac
841 ttatccttga cagctctgtt tgggaagcag gacaaagcta catgtcagga aactgtggag
901 cctccgcaga ctctccacca gcagcagcag cagcagcagc agcagcaaga gaagcttcca
961 attaggcagg gggttgtacg ctccctgtcc tatgaggaac ccagaagaca ctcacccccc
1021 attgagaagc agctctgtcc agccattcag aaactcatgg tcaggagcgc agacctccac
1081 ccattgtcag agctgcctga aaaccggcct tgtgaaaatg gcagtaccca ttctgcggga
1141 gaatttttta caggacctgt ccagccaggg tctcctcaca acattggaac ttctcgtggt
1201 gtacaaaatg cttccagaac tcagaacctg ttcgagaaac ttcagagtac cccaggggca
1261 gcaaacaagt gtgaccctag tacaccagca cctgccagct cagctgccct gaaccgcagc
1321 agagctccca cttctgtcac ccctgtggct ccaggaaagg gtctggctca gccaccacag
1381 gcctatttca atggctccct tccacctcag acagtaggac atcaggctca tggaagagaa
1441 cagtccacac tcccaagaca aacactcccc atctctggta gtcagactgg cagctctgga
1501 gtgatctccc ctcaagagtt actgaagaag cttcagattg tacagcagga gcagcagctg
1561 catgcctcta accggccagc cttggccgct aagtttcctg tgctcgctca gagctctgga
1621 acagggaaac ccttggaatc ctggatcaac aagacaccca acacagaaca gcagactcct
1681 cttttccagg tcatctcacc tcagcgcatc cctgctacag cagctccgtc tctgcttatg
1741 tcccccatgg tgttcgcaca acccacctcc gtcccgccaa aggaaaggga gagcggcctc
1801 ttgcctgtgg gaggccagga gccacctgct gctgccacca gcctcctcct gcccatacag
1861 agcccggagc cctccgtgat caccagcagc ccactcacca agctccagct ccaggaggca
1921 ctgctgtacc tcattcagaa tgatgacaac ttcttaaata taatctatga agcctatctc
1981 ttcagcatga ctcaagcagc catgaaaaag actatgtgac agcaaggcct tttaaaactg
2041 attttcaagg tccttctaga actccggcac aagtgcagag tggacctcca gcaaactcca
2101 gcagacctgc agcagagggg cctgactgct agaaggaaaa ctaacaaaca gaaaggaata
2161 ccatcaacat taacaaaaag gacgtccacg caaaaacccc atccgaaggt catcagtgtc
2221 aaagataaat ccacgaagat ggggaaaaaa cagtgcaaaa aggctaaaaa ttcc
//