LOCUS AK299198 1553 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ50047 complete cds.
ACCESSION AK299198
VERSION AK299198.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1553)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1553
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7007099"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000493822"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 243..1286
/codon_start=1
/protein_id="BAG61240.1"
/transl_table=1
/translation="MARFWVCVAGAGFFLAFLVLHSRFCGSPVLRNFTFAVSWRTEKI
LYRLDVGWPKHPEYFTGTTFCVAVDSLNGLVYIGQRGDNVPKILVFTEDGYFLRAWNY
TVDTPHGIFAASTLYEQSVWITDVGSGFFGHTVKKYSSFGDLVQVLGTPGKKGTSLNP
LQFDNPAELYVEDTGDIYIVDGDGGLNNRLIKLSQDFMILWLHGENGTGPAKFNIPHS
VTLDSAGRVWVADRGNKRIQVFDKDTGEWLGAWNNCFTEEGPSSVRFTPDGKYLIVAQ
LNLSRLSVVAAPPVGSIGECSVISTIQLADQVLPHLLEVDRKTGAVYVAEIGAKQVQK
YVPLNSYVPSFGS"
BASE COUNT 408 a 305 c 379 g 461 t
ORIGIN
1 ttgagggcgg ggaccccgaa atgcacacga agtccggaac tggtcttctg tgattttcat
61 tcgccctggt ctctgttccc tttcgtactc aaagctcgtg catccaggga ggggaaaccg
121 gagatagggt cttcgggccc cgggcagacc ctctgtgccg ctgcaaaccg ttgcagcctg
181 aggctgtcag gtcctccccc agacacctgc ggaccctccc tctcctggct tcccgtctgg
241 tcatggcgag attctgggtc tgcgtagccg gtgctggctt ctttcttgca tttttggttt
301 tgcattcgcg tttttgtggc tctccagttt tgaggaactt tacttttgca gtttcctgga
361 gaactgagaa aattctttac cggctggatg tgggttggcc taagcaccca gaatatttta
421 ccggaacaac attttgtgtt gcagttgact ccctcaatgg attggtttac ataggtcaaa
481 gaggggataa cgtcccaaag atattagtgt tcacagagga tggatatttc ctacgagcct
541 ggaattatac agttgacaca cctcatggta tatttgcagc cagtactcta tatgaacaat
601 ccgtctggat cacggatgta ggaagtggat tctttggtca tactgttaaa aaatacagtt
661 cttttggtga tcttgttcaa gtcttgggta ctccaggcaa aaaaggcact agtttgaatc
721 ctttgcagtt tgataaccca gcagaattat atgtagagga cacaggagat atttacattg
781 tggatggaga tggaggattg aataacagat tgatcaaact gtcccaagat ttcatgatcc
841 tttggctgca tggagaaaat gggacagggc ctgctaagtt caacatacct cacagtgtta
901 cacttgattc agctggtcgg gtgtgggttg ctgaccgagg aaataaaaga atccaagtat
961 ttgataaaga cactggggag tggttaggag catggaataa ttgtttcaca gaagagggac
1021 cttcttcagt caggtttact cctgatggga agtacttgat tgtggcccag ctgaatctta
1081 gcaggctctc agtcgtagca gcacccccag tgggaagcat tggggagtgt tctgtgatca
1141 gcacaatcca actagcagat caagttttgc cacatctcct agaagtcgac agaaagactg
1201 gagcagtcta tgtagcagaa attggagcaa aacaagtaca aaaatatgtc cctttgaata
1261 gctatgttcc ttcatttggt tcataatgtt tctttcccgg gaatatttca agtggcagtt
1321 cagattctca attcactaag tgcttaaaaa tgatgttcaa gcacaagaat ttatttttct
1381 agtataaaag atctagtatc agaaagattt gtttttgtat cattaagaat cttatatttt
1441 gttgccctct tgggacttag ttttatttgt aagtgcataa ggatatttta atgaaaggaa
1501 agtaactaaa aaatggggtt gggaagaggg actaaggtgg taacctcatt att
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