LOCUS AK299185 2634 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ59395 complete cds, highly similar to Homo
sapiens sphingomyelin phosphodiesterase 4, neutral membrane,
transcript variant 1, mRNA.
ACCESSION AK299185
VERSION AK299185.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2634)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2634
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7002661"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000493809"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 7..2301
/codon_start=1
/note="highly similar to Homo sapiens sphingomyelin
phosphodiesterase 4, neutral membrane, transcript variant
1, mRNA"
/protein_id="BAG61230.1"
/transl_table=1
/translation="MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAMAFPH
LQQPSFLLASLKADSINKPFAQQCQDLVKVIEDFPAKGPVKASIQECILPDSPLYHNK
VQFTPTGGLGLNLALNPFEYYIFFFALGLITQKPLPVSLHVRTSDCAYFILVDRYLSW
FLPTEGSVPPPLSSSPGGTSPSPPPRTPAIPFASYGLHHTSLLKRHISHQTSVNADPA
SHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKESFTPTEEHVLVVRLLLKHLHA
FANSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAV
LEMWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNRALRT
DLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLFTAPTFTGSF
LSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQLITQAKHTAKSI
SDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQI
FRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEY
QGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPG
LASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCTLL
LTLGYVLYASAMTLLTERGELHQP"
BASE COUNT 519 a 868 c 709 g 538 t
ORIGIN
1 agcttgatga cgactttcgg cgccgtggcg gaatggcggc ttccatctct gaggcgagcg
61 acgctatgga tcccacagtg gtttgctaag aaggccattt tcaactctcc actggaggct
121 gctatggcgt tccctcacct gcagcagccc agctttctac tggctagcct gaaagctgac
181 tctataaata agccctttgc acagcagtgc caagacttgg ttaaagtcat tgaggacttt
241 ccagcaaagg gtcctgtgaa ggcgtccatc caggagtgca tcctccctga cagtcctctg
301 taccacaaca aggtccagtt cacccctact gggggccttg gtctgaactt ggccctgaat
361 ccgttcgagt attacatatt cttctttgcc ttgggcctca tcactcagaa gccacttcct
421 gtgtccctcc acgtccgtac ttcagactgc gcctatttca tcctggtgga caggtacctg
481 tcatggttcc tgcccaccga aggcagtgtg cccccaccac tctcctccag cccagggggg
541 accagcccct caccacctcc caggacacca gccataccct ttgcttccta tggcctccac
601 cacactagcc tcctaaagcg acacatctct catcagacgt ctgtgaatgc agaccccgcc
661 tcccacgaga tctggaggtc agaaactctg ctccaggttt ttgttgaaat gtggcttcat
721 cactattcct tggagatgta tcaaaaaatg cagtcccctc atgccaagga gtcgttcacg
781 cctactgagg agcatgtgtt ggtggtgcgc ctgctgctga agcacctgca cgcctttgcc
841 aacagcctga agccagagca ggcctcaccc tccgcccact cccacgccac cagccccctg
901 gaggagttca aacgggctgc tgtcccgagg ttcgtccagc agaaactcta cctcttcttg
961 cagcattgct ttggccactg gcccctggac gcatcgttca gagctgtcct ggagatgtgg
1021 ctgagctacc tgcagccgtg gcggtacgcg cctgacaagc aggctccggg cagcgactcc
1081 cagccccggt gtgtgtcgga gaaatgggca ccctttgtcc aggagaacct gctgatgtac
1141 accaagttgt ttgtgggctt tctgaaccgc gcgctccgca cagacctggt cagccccaag
1201 cacgcgctca tggtgttccg agtggccaaa gtctttgccc agcccaacct ggctgagatg
1261 attcagaaag gtgagcagct attcctggag ccagagctgg tcatccccca ccgccagcac
1321 cgactcttca cggcccccac attcactggg agcttcctgt caccctggcc accagcggtc
1381 actgatgcct ccttcaaggt gaagagccac gtctacagcc tggagggcca ggactgcaag
1441 tacaccccga tgtttgggcc cgaggcccgc accctggtcc tgcgcctcgc tcagctcatc
1501 acacaggcca aacacacagc caagtccatc tccgaccagt gtgcggagag cccggctggc
1561 cactccttcc tctcatggct gggctttagc tccatggaca ccaatggctc ctacacagcc
1621 aacgacctgg acgagatggg gcaagacagt gtccggaaga cagatgaata cctggagaag
1681 gccctggagt acctgcgcca gatattccgg ctcagcgaag cgcagctcag gcagttcaca
1741 ctcgccttgg gcaccaccca ggatgagaat ggaaaaaagc aactccccga ctgcatcgtg
1801 ggtgaggacg gactcatcct tacgcccctg gggcggtacc agatcatcaa tgggctgcga
1861 aggtttgaaa ttgagtacca gggggacccg gagctgcagc ccatccggag ctatgagatc
1921 gccagcttgg tccgcacact ctttaggctg tcgtctgcca tcaaccacag atttgcagga
1981 cagatggcgg ctctgtgttc ccgggatgac ttcctcggca gcttctgtcg ctaccacctc
2041 acagaacctg ggctggccag caggcacctg ctgagccctg tggggcggag gcaggtggcc
2101 ggccacaccc gcggccccag gctcagcctg cgcttcctgg gcagttaccg gacgctggtc
2161 tcgctgctgc tggccttctt cgtggcctct ctgttctgcg tcgggcccct cccatgcacg
2221 ctgctgctca ccctgggcta tgtcctctac gcctctgcca tgacactgct gaccgagcgg
2281 ggggagctgc accagccctg aaggtgtcag ctgccttcag agcaggctgg agggatttgc
2341 cacacagccc cacccttggg ctgagaggac ctgggaagcc cctccaggag ggaacacggt
2401 catcctcggg cttctggagc ggggttcctg cagccgcaga ggcatctgga ggaaacgcaa
2461 ccaagaaagg aaggcaggtg ggccccagca aaggagtagc tgccagggct caacagctac
2521 gctctgtgac agcgcagagc tcagcggcgg cctttccctc cctccgccaa ggactcacgg
2581 ccaagccagc tctcggggcc ttttttccac tgcccatttg gctactctgc tgca
//