LOCUS AK299184 1809 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ54136 complete cds, highly similar to
Proactivator polypeptide precursor.
ACCESSION AK299184
VERSION AK299184.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1809)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1809
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7001655"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000493808"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 65..1648
/codon_start=1
/note="highly similar to Proactivator polypeptide
precursor"
/protein_id="BAG61229.1"
/transl_table=1
/translation="MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGA
VKHYLQTVWNKPTVKSLPCVICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM
SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP
ELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF
VQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQDQQPKEICALVGFCDE
VKEMPMQTLVPAKVASKDVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDN
NKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS
GTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEIPAALEKGCSFLPDPY
QKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQN
TETAAQCNAVEHCKRHVWN"
BASE COUNT 423 a 474 c 512 g 400 t
ORIGIN
1 agatttatat ctgcggggga tcagctgacg ctccgcattg cagactgcgg agtcagacgg
61 cgctatgtac gccctcttcc tcctggccag cctcctgggc gcggctctag ccggcccggt
121 ccttggactg aaagaatgca ccaggggctc ggcagtgtgg tgccagaatg tgaagacggc
181 gtccgactgc ggggcagtga agcactacct gcagaccgtt tggaacaagc caacagtgaa
241 atcccttccc tgcgtcatat gcaaagacgt tgtcaccgca gctggtgata tgctgaagga
301 caatgccact gaggaggaga tccttgttta cttggagaag acctgtgact ggcttccgaa
361 accgaacatg tctgcttcat gcaaggagat agtggactcc tacctccctg tcatcctgga
421 catcattaaa ggagagatga gccgtcctgg ggaggtgtgc tctgctctca acctctgcga
481 gtctctccag aagcacctag cagagctgaa tcaccagaag cagctggagt ccaataagat
541 cccagagctg gacatgactg aggtggtggc ccccttcatg gccaacatcc ctctcctcct
601 ctaccctcag gacggccccc gcagcaagcc ccagccaaag gataatgggg acgtttgcca
661 ggactgcatt cagatggtga ctgacatcca gactgctgta cggaccaact ccacctttgt
721 ccaggccttg gtggaacatg tcaaggagga gtgtgaccgc ctgggccctg gcatggccga
781 catatgcaag aactatatca gccagtattc tgaaattgct atccagatga tgatgcacat
841 gcaggatcag caacccaagg agatctgtgc gctggttggg ttctgtgatg aggtgaaaga
901 gatgcccatg cagactctgg tccccgccaa agtggcctcc aaggatgtca tccctgccct
961 ggaactggtg gagcccatta agaagcacga ggtcccagca aagtctgatg tttactgtga
1021 ggtgtgtgaa ttcctggtga aggaggtgac caagctgatt gacaacaaca agactgagaa
1081 ggaaatactc gacgcttttg acaaaatgtg ctcgaagctg ccgaagtccc tgtcggaaga
1141 gtgccaggag gtggtggaca cgtacggcag ctccatcctg tccatcctgc tggaggaggt
1201 cagccctgag ctggtgtgca gcatgctgca cctctgctct ggcacgcggc tgcctgcact
1261 gaccgttcac gtgactcagc caaaggacgg tggcttctgc gaagtgtgca agaagctggt
1321 gggttatttg gatcgcaacc tggagaaaaa cagcaccaag caggagatcc cggctgctct
1381 tgagaaaggc tgcagcttcc tgccagaccc ttaccagaag cagtgtgatc agtttgtggc
1441 agagtacgag cccgtgctga tcgagatcct ggtggaggtg atggatcctt ccttcgtgtg
1501 cttgaaaatt ggagcctgcc cctcggccca taagcccttg ttgggaactg agaagtgtat
1561 atggggccca agctactggt gccagaacac agagacagca gcccagtgca atgctgtcga
1621 gcattgcaaa cgccatgtgt ggaactagga ggaggaatat tccatcttgg cagaaaccac
1681 agcattggtt tttttctact tgtgtgtctg ggggaatgaa cgcacagatc tgtttgactt
1741 tgttataaaa atagggctcc cccacctccc ccatttctgt gtcctttatt gtagcattgc
1801 tgtctgcaa
//