LOCUS AK299179 3015 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ52431 complete cds, highly similar to
Retinoblastoma-associated protein.
ACCESSION AK299179
VERSION AK299179.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3015)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3015
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP6000383"
/clone_lib="NT2RP6"
/db_xref="H-InvDB:HIT000493803"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 414..2864
/codon_start=1
/note="highly similar to Retinoblastoma-associated
protein"
/protein_id="BAG61225.1"
/transl_table=1
/translation="MSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYD
VLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVI
SFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTR
IIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKD
LDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMI
LNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRY
KLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQ
NLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLA
WLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTT
RVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEH
IIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQ
ETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPS
SPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMV
CNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTR
MQKQKMNDSMDTSNKEEK"
BASE COUNT 984 a 594 c 596 g 841 t
ORIGIN
1 agttgccggg cgggggaggg cgcgtccggt ttttctcagg ggacgttgaa attatttttg
61 taacgggagt cgggagagga cggggcgtgc cccgacgtgc gcgcgcgtcg tcctccccgg
121 cgctcctcca cagctcgctg gctcccgccg cggaaaggcg tcatgccgcc caaaaccccc
181 cgaaaaacgg cccggaggac ctgcctctcg tcaggcttga gtttgaagaa acagaagaac
241 ctgattttac tgcattatgt cagaaattaa agataccaga tcatgtcaga gagagagctt
301 ggttaacttg ggagaaagtt tcatctgtgg atggagtatt gggaggttat attcaaaaga
361 aaaaggaact gtggggaatc tgtatcttta ttgcagcagt tgacctagat gagatgtcgt
421 tcacttttac tgagctacag aaaaacatag aaatcagtgt ccataaattc tttaacttac
481 taaaagaaat tgataccagt accaaagttg ataatgctat gtcaagactg ttgaagaagt
541 atgatgtatt gtttgcactc ttcagcaaat tggaaaggac atgtgaactt atatatttga
601 cacaacccag cagttcgata tctactgaaa taaattctgc attggtgcta aaagtttctt
661 ggatcacatt tttattagct aaaggggaag tattacaaat ggaagatgat ctggtgattt
721 catttcagtt aatgctatgt gtccttgact attttattaa actctcacct cccatgttgc
781 tcaaagaacc atataaaaca gctgttatac ccattaatgg ttcacctcga acacccaggc
841 gaggtcagaa caggagtgca cggatagcaa aacaactaga aaatgataca agaattattg
901 aagttctctg taaagaacat gaatgtaata tagatgaggt gaaaaatgtt tatttcaaaa
961 attttatacc ttttatgaat tctcttggac ttgtaacatc taatggactt ccagaggttg
1021 aaaatctttc taaacgatac gaagaaattt atcttaaaaa taaagatcta gatgcaagat
1081 tatttttgga tcatgataaa actcttcaga ctgattctat agacagtttt gaaacacaga
1141 gaacaccacg aaaaagtaac cttgatgaag aggtgaatgt aattcctcca cacactccag
1201 ttaggactgt tatgaacact atccaacaat taatgatgat tttaaattca gcaagtgatc
1261 aaccttcaga aaatctgatt tcctatttta acaactgcac agtgaatcca aaagaaagta
1321 tactgaaaag agtgaaggat ataggataca tctttaaaga gaaatttgct aaagctgtgg
1381 gacagggttg tgtcgaaatt ggatcacagc gatacaaact tggagttcgc ttgtattacc
1441 gagtaatgga atccatgctt aaatcagaag aagaacgatt atccattcaa aattttagca
1501 aacttctgaa tgacaacatt tttcatatgt ctttattggc gtgcgctctt gaggttgtaa
1561 tggccacata tagcagaagt acatctcaga atcttgattc tggaacagat ttgtctttcc
1621 catggattct gaatgtgctt aatttaaaag cctttgattt ttacaaagtg atcgaaagtt
1681 ttatcaaagc agaaggcaac ttgacaagag aaatgataaa acatttagaa cgatgtgaac
1741 atcgaatcat ggaatccctt gcatggctct cagattcacc tttatttgat cttattaaac
1801 aatcaaagga ccgagaagga ccaactgatc accttgaatc tgcttgtcct cttaatcttc
1861 ctctccagaa taatcacact gcagcagata tgtatctttc tcctgtaaga tctccaaaga
1921 aaaaaggttc aactacgcgt gtaaattcta ctgcaaatgc agagacacaa gcgacctcag
1981 ccttccagac ccagaagcca ttgaaatcta cctctctttc actgttttat aaaaaagtgt
2041 atcggctagc ctatctccgg ctaaatacac tttgtgaacg ccttctgtct gagcacccag
2101 aattagaaca tatcatctgg acccttttcc agcacaccct gcagaatgag tatgaactca
2161 tgagagacag gcatttggac caaattatga tgtgttccat gtatggcata tgcaaagtga
2221 agaatataga ccttaaattc aaaatcattg taacagcata caaggatctt cctcatgctg
2281 ttcaggagac attcaaacgt gttttgatca aagaagagga gtatgattct attatagtat
2341 tctataactc ggtcttcatg cagagactga aaacaaatat tttgcagtat gcttccacca
2401 ggccccctac cttgtcacca atacctcaca ttcctcgaag cccttacaag tttcctagtt
2461 cacccttacg gattcctgga gggaacatct atatttcacc cctgaagagt ccatataaaa
2521 tttcagaagg tctgccaaca ccaacaaaaa tgactccaag atcaagaatc ttagtatcaa
2581 ttggtgaatc attcgggact tctgagaagt tccagaaaat aaatcagatg gtatgtaaca
2641 gcgaccgtgt gctcaaaaga agtgctgaag gaagcaaccc tcctaaacca ctgaaaaaac
2701 tacgctttga tattgaagga tcagatgaag cagatggaag taaacatctc ccaggagagt
2761 ccaaatttca gcagaaactg gcagaaatga cttctactcg aacacgaatg caaaagcaga
2821 aaatgaatga tagcatggat acctcaaaca aggaagagaa atgaggatct caggaccttg
2881 gtggacactg tgtacacctc tggattcatt gtctctcaca gatgtgactg tataactttc
2941 ccaggttctg tttatggcca catttaatat cttcagctct ttttgtggat ataaaatgtg
3001 cagatgcaat tgttt
//