LOCUS AK299151 2255 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58252 complete cds, highly similar to Homo
sapiens DEAH (Asp-Glu-Ala-His) box polypeptide 40 (DHX40), mRNA.
ACCESSION AK299151
VERSION AK299151.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2255)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2255
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2004549"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000493775"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 274..2169
/codon_start=1
/note="highly similar to Homo sapiens DEAH
(Asp-Glu-Ala-His) box polypeptide 40 (DHX40), mRNA"
/protein_id="BAG61200.1"
/transl_table=1
/translation="MTDGCLLKHILGDPNLTKFSVIILDEAHERTLTTDILFGLLKKL
FQEKSPNRKEHLKVVVMSATMELAKLSAFFGNCLIFDIPGRLYPVREKFCNLIGPRDR
ENTAYIQAIVKVTMDIHLNEMAGDILVFLTGQFEIEKSCELLFQMAESVDYDYDVQDT
TLDGLLILPCYGSMTTDQQRRIFLPPPPGIRKCVISTNISATSLTIDGIRYVVDGGFV
KQLNHNPRLGLDILEVVPISKSEALQRSGRAGRTSSGKCFRIYSKDFWNQCMPDHVIP
EIKKTSLTSVVLTLKCLAIHDVIRFPYLDPPNERLILEALKQLYQCDAIDRSGHVTRL
GLSMVEFPLPPHLTCAVIKAASLDCEDLLLPIAAMLSVENVFIRPVDPEYQKEAEQRH
RELAAKAGGFNDFATLAVIFEQCKSSGAPASWCQKHWIHWRCLFSAFRVEAQLRELIR
KLKQQSDFPKETFEGPKHEVLRRCLCAGYFKNVARRSVGRTFCTMDGRGSPVHIHPSS
ALHEQETKLEWIIFHEVLVTTKVYARIVCPIRYEWVRDLLPKLHEFNAHDLSSVARRE
VREDARRRWTNKENVKQLKDGISKDVLKKMQRRNDDKSISDARARFLERKQQRTQDHS
DTRKETG"
BASE COUNT 686 a 437 c 534 g 598 t
ORIGIN
1 ctttcccctc ccatctcctc agatcggtgg acgtgctcgc ctccactcgg ggccaggtct
61 atgtcccggt ttcccgcagt cgcgggcagg gcgccaaggc ggcaggagga gggtgagcgg
121 tcaagagacc tccaggaaga gcggctctcg gctgtttgca tcgccgatag agaatagaaa
181 ggatgcacgt cccaggaggg aggaactact ccaacttttc ctattcagaa acaaagaaaa
241 aagattattc aagctgagac agcaatcaaa tatatgactg atggatgttt actgaaacat
301 attctgggag acccaaatct taccaaattc agtgtcatta ttttggatga agcccatgaa
361 agaactctaa ctacagatat cttatttggt ttattgaaga agctatttca ggagaagtct
421 cctaatagga aggagcattt aaaagtggtg gtaatgtcag caactatgga attagccaag
481 ctctctgcat tctttggaaa ttgtctaata tttgatatac ctggaaggct ttatccagtc
541 agagagaaat tctgcaattt gattggtccg cgagacagag aaaatactgc gtatattcaa
601 gcgattgtga aagtcaccat ggatatccat ttgaatgaaa tggctggaga catcttggtt
661 tttctgactg gccagtttga aatagaaaaa agttgtgagt tactttttca gatggcagag
721 tctgttgatt atgattatga tgttcaagat accaccctcg atggcttgtt aatattgccg
781 tgttatggat caatgacaac agatcaacag aggagaatat ttttgccacc accacctgga
841 attagaaaat gtgtcatatc caccaatatt tctgcaacgt ctttgacaat agatggaatc
901 agatatgtgg tagatggtgg cttcgtgaag cagttaaatc acaaccccag attagggttg
961 gacatcctgg aggtggttcc aatttcaaag agcgaggcat tacagcgaag tggccgagct
1021 ggcaggactt cttcaggaaa atgctttcgg atctatagta aagatttttg gaaccagtgt
1081 atgcctgacc atgtgatccc tgaaattaag aaaactagtt tgacatctgt agttctgacc
1141 ttaaagtgcc ttgccataca cgatgtcata aggtttccct atttggatcc acctaatgag
1201 agacttattt tagaagctct taaacaactt taccagtgtg atgctattga caggagtggc
1261 catgtcacca gattgggttt gtctatggtg gagtttcctt tgcctccaca tctgacatgt
1321 gcagtaataa aagctgcttc cctggattgt gaagatctac tacttccaat agcagcaatg
1381 ttgtctgtgg aaaacgtctt cattagacct gttgatccag agtaccagaa ggaagcagaa
1441 cagagacatc gagaattggc agctaaagct ggaggattta atgactttgc aactttagct
1501 gtcatctttg aacaatgcaa atcaagtgga gctccagctt catggtgcca aaaacactgg
1561 attcattgga ggtgcttatt ttctgcattt cgtgtggaag ctcaacttcg agaactaatc
1621 aggaagctta aacagcaaag tgatttccca aaagagacct ttgaaggccc taaacatgaa
1681 gtactacgaa gatgtctttg tgcgggctat ttcaaaaatg tagctcgaag atctgttggg
1741 agaacgtttt gcacaatgga tggtcgtgga agcccagttc acattcatcc ttcctcagca
1801 cttcatgaac aggaaaccaa acttgaatgg atcatttttc atgaggtatt ggttaccacc
1861 aaagtctacg caagaattgt atgcccaatc cgttatgaat gggtaagaga cttgttaccc
1921 aagttgcatg aatttaatgc acatgatttg agcagtgtgg cccgacgtga agtgagagaa
1981 gatgcaagaa ggagatggac aaataaggaa aatgtaaagc agctaaagga tggaatatcg
2041 aaagacgtct taaagaaaat gcaaagaaga aatgatgaca aatccatatc tgatgcacgg
2101 gctcgtttcc ttgagagaaa gcagcagagg acccaggacc acagtgacac acgaaaggaa
2161 acaggctaag gtggtgaacc ctccaattca ggaggtggga aaaggagcca ggaaatgtgc
2221 ttctactttg ccagttattt cagacagcac tacca
//