LOCUS AK299145 2346 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50625 complete cds, highly similar to
Importin beta-1 subunit.
ACCESSION AK299145
VERSION AK299145.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2346)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2346
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2001029"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000493769"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 88..2070
/codon_start=1
/note="highly similar to Importin beta-1 subunit"
/protein_id="BAH12957.1"
/transl_table=1
/translation="MKESTLESIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNN
VKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSL
YYQYMETYMGPALFADENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIK
NPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGR
ICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQE
EPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKT
TLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVM
ASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQV
CLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAI
GGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQEN
VHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEAR
PMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA"
BASE COUNT 672 a 493 c 596 g 585 t
ORIGIN
1 tgcttgtgca gagatccagt aaaccagtgg ccagaactca ttcctcagct ggtggccaat
61 gtcacaaacc ccaacagcac agagcacatg aaggagtcga cattggaatc catcggttat
121 atttgccaag atatagaccc agagcagcta caagataaat ccaatgagat tctgactgcc
181 ataatccagg ggatgaggaa agaagagcct agtaataatg tgaagctagc tgctacgaat
241 gcactcctga actcattgga gttcaccaaa gcaaactttg ataaagagtc tgaaaggcac
301 tttattatgc aggtggtctg tgaagccaca cagtgtccag atacgagggt acgagtggct
361 gctttacaga atctggtgaa gataatgtcc ttatattatc agtacatgga gacatatatg
421 ggtcctgctc tttttgcaga cgaaaatgat gatgacgatg actggaaccc ctgcaaagca
481 gcaggggtgt gcctcatgct tctggccacc tgctgtgaag atgacattgt cccacatgtc
541 ctccccttca ttaaagaaca catcaagaac ccagattggc ggtaccggga tgcagcggtg
601 atggcttttg gttgtatctt ggaaggacca gagcccagtc agctcaaacc actagttata
661 caggctatgc ccaccctaat agaattaatg aaagacccca gtgtagttgt tcgagataca
721 gctgcatgga ctgtaggcag aatttgtgag ctgcttcctg aagctgccat caatgatgtc
781 tacttggctc ccctgctaca gtgtctgatt gagggtctca gtgctgaacc cagagtggct
841 tcaaatgtgt gctgggcttt ctccagtctg gctgaagctg cttatgaagc tgcagacgtt
901 gctgatgatc aggaagaacc agctacttac tgcttatctt cttcatttga actcatagtt
961 cagaagctcc tagagactac agacagacct gatggacacc agaacaacct gaggagttct
1021 gcatatgaat ctctgatgga aattgtgaaa aacagtgcca aggattgtta tcctgctgtc
1081 cagaaaacga ctttggtcat catggaacga ctgcaacagg ttcttcagat ggagtcacat
1141 atccagagca catccgatag aatccagttc aatgaccttc agtctttact ctgtgcaact
1201 cttcagaatg ttcttcggaa agtgcaacat caagatgctt tgcagatctc tgatgtggtt
1261 atggcctccc tgttaaggat gttccaaagc acagctgggt ctgggggagt acaagaggat
1321 gccctgatgg cagttagcac actggtggaa gtgttgggtg gtgaattcct caagtacatg
1381 gaggccttta aacccttcct gggcattggg ttaaaaaatt atgctgaata ccaggtctgt
1441 ttggcagctg tgggcttagt gggagacttg tgccgtgccc tgcaatccaa catcatacct
1501 ttctgtgacg aggtgatgca gctgcttctg gaaaatttgg ggaatgagaa cgtccacagg
1561 tctgtgaagc cgcagattct gtcagtgttt ggtgatattg cccttgctat tggaggagag
1621 tttaaaaaat acttagaggt tgtattgaat actcttcagc aggcctccca agcccaggtg
1681 gacaagtcag actatgacat ggtggattat ctgaatgagc taagggaaag ctgcttggaa
1741 gcctatactg gaatcgtcca gggattaaag ggggatcagg agaacgtaca cccggatgtg
1801 atgctggtac aacccagagt agaatttatt ctgtctttca ttgaccacat tgctggagat
1861 gaggatcaca cagatggagt agtagcttgt gctgctggac taatagggga cttatgtaca
1921 gcatttggga aggatgtact gaaattagta gaagctaggc caatgatcca tgaattgtta
1981 actgaagggc ggagatcgaa gactaacaaa gcaaaaaccc ttgctacatg ggcaacaaaa
2041 gaactgagga aactgaagaa ccaagcttga tctgttacca ttgggatgat aacctgagga
2101 cccccactgg aaatctccca tcttttgaaa aacctggaag tgaggagtgt gcacggatgc
2161 tgaatgtttg ggaatgagag gatgagtgag tgaggcttga aaacacacca cattgaaaat
2221 cctgccacag cagcagccgc agccgccaac agcagcgctg ttagtgagct aagtaagcac
2281 tgacttcgta gaaaaccata acatcggcca tcttggaaaa gagaaaaaca atggagttac
2341 ttattt
//