LOCUS AK299108 1047 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ56787 complete cds, highly similar to Histone
H2B type 2-F.
ACCESSION AK299108
VERSION AK299108.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1047)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1047
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3008235"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493732"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 48..548
/codon_start=1
/note="highly similar to Histone H2B type 2-F"
/protein_id="BAG61164.1"
/transl_table=1
/translation="MPDPAKSAPAPKKGSKKAVTKVQKKDGKKRKRSRKESYSVYVYK
VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLL
LPGELAKHAVSEGTKAVTKYTSSNPRNLSPTKPGGSEDRQPPPSQLSAIPPFCLVLRA
GIAGQV"
BASE COUNT 238 a 329 c 256 g 224 t
ORIGIN
1 agttcggatt acttctttaa gtctcttttc tcttttttcg cgcaaaaatg ccggatccag
61 cgaaatccgc tcctgctccc aagaagggct ccaaaaaggc tgttacgaaa gtgcagaaga
121 aggacggcaa gaagcgcaag cgcagccgca aggagagcta ctccgtttac gtgtacaagg
181 tgctgaagca ggtccacccc gacaccggca tctcgtccaa ggccatgggc atcatgaact
241 ccttcgtcaa cgacatcttc gagcgcatcg cgggagaggc gtcccgcctg gcgcactaca
301 acaagcgctc caccatcaca tcccgcgaga tccagacggc cgtgcgcctg ctgctgcccg
361 gcgagctggc caagcacgcc gtgtccgagg gcaccaaggc ggtcaccaag tacaccagct
421 cgaacccgag gaatctgtct ccaactaagc ctggtggcag tgaggaccgt cagccccctc
481 ccagccagct gtcagctata cctccattct gtctggttct cagggctgga atcgctgggc
541 aggtctagct aatccttggc cctcctagga aaatattcct aaatcctgaa ggcatttagc
601 taggaaagat ccagaatgga cccaactctt cctgttttac cccaaaccgg ttggtctgct
661 ttggggatta cttctcaggc taggaacctc tgagtgttct gaaacgggcc tgaactctta
721 cagaagctct tgtggcccta ggatgaattt agaggtctcc atacccagcc tggaaggtga
781 agaacgaccc ctaacgaccc accctcccaa cctaatgccg gttctgctcc acttgtccac
841 tctggggagc tgtgctttgg cttggaagct ctcggattga aggatgggcc caagaccctc
901 acaggtgaag cctcggtagg tcccagggag aagaaagatt cctccacccc accacccctc
961 taagtccgac ccacctttct ccccgccagc tgcatcagat ccctccaggg cctcattgtc
1021 agtcctgtaa tcttttcatc cgtgata
//