LOCUS AK299097 971 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ53771 complete cds, highly similar to
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4.
ACCESSION AK299097
VERSION AK299097.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 971)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..971
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3007255"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493721"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 48..476
/codon_start=1
/note="highly similar to
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4"
/protein_id="BAH12949.1"
/transl_table=1
/translation="MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVM
VGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKI
RKFLMPQTPPENGERPSLILENRMATRPFLSSPSVWILRS"
BASE COUNT 227 a 272 c 275 g 197 t
ORIGIN
1 tgctgctggg ttggaagcgg cgcagtccga ctcatcccgg ccccgggatg gcgtccccac
61 gggaattgac acagaacccc ctgaagaaga tctggatgcc atacagcaat gggcggcccg
121 ctctgcacgc ttgccagcgc ggtgtgtgca tgaccaactg cccaactctc attgtcatgg
181 tgggcctgcc cgccaggggc aagacctaca tctccaagaa gctgactcga tacctgaact
241 ggattggtgt gcccactcgg gagttcaatg ttggccagta tcgccgggac gtggtcaaga
301 cctacaaatc ttttgaattt tttctccccg acaatgaaga gggcctgaaa atcaggaagt
361 ttttgatgcc acaaacacca cccgagaacg gagagcgacc atctttaatt ttggagaaca
421 gaatggctac aagacctttt ttgtcgagtc catctgtgtg gatcctgagg tcatagctgc
481 caacatcgtg caagtgaaac tgggcagccc tgactatgtc aaccgcgaca gtgatgaggc
541 tacggaggac ttcatgaggc gcattgagtg ctatgagaac tcctacgagt cgctagatga
601 ggacctggat agggacctgt cctatatcaa gatcatggat gtgggccaga gctacgtggt
661 gaaccgtgtg gctgaccaca tccagagccg catcgtatat tacctcatga acatccacgt
721 gaccccccgc tccatctacc tctgccggca cggggagagc gagctcaacc tcaagggccg
781 gattggcggg gacccaggac tgtcccctcg gggcagggag ggcgtctgtg aggaaatgac
841 ctacgaggaa attcaggata attatccact ggagttcgcc ctgcgggacc aggacaagta
901 ccggtaccgg taccctaaag gggaggctga gcccatcctg tccttcctgt ggctccaggc
961 accaccccca g
//