LOCUS AK299093 1227 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50624 complete cds.
ACCESSION AK299093
VERSION AK299093.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1227)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1227
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3006854"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493717"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 6..602
/codon_start=1
/protein_id="BAH12948.1"
/transl_table=1
/translation="MLEDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLST
HSPFRTLNINGQIPTGEGPPLVKTQTVTISDNANAVKSEIPTKDVPIVHTETKTITYE
AAQTDDNSGDLDPGVLLTAQTITSETPSSTTTTQITKTVKGGISETRIEKRIVITGDA
DIDHDQVLVQAIKEAKEQHPDMSVTKVVVNQETEIADE"
BASE COUNT 362 a 299 c 263 g 303 t
ORIGIN
1 atttaatgtt ggaggattta gacaagagtc aagaggagat caaaaaacat catgccagca
61 tcagtgagct gaaaaagaac ttcatggagt ctgtaccaga accacggcct agtgaatggg
121 ataaacgctt atccactcac tcacccttcc gaactcttaa catcaatggg caaatcccca
181 caggagaagg acctcccctg gtgaagacac aaactgtcac catctcagat aatgccaatg
241 ctgtgaaaag tgaaatccca accaaagacg tccctattgt ccacactgag accaagacca
301 tcacttatga ggctgcccag actgacgaca acagtggaga cttggaccca ggagtcttgc
361 tgacagctca aactatcaca tctgagaccc caagcagcac caccacaact caaattacca
421 agactgtaaa aggtgggatt tcagagacac gtattgaaaa gagaattgtg atcacaggag
481 atgctgatat tgaccatgat caggtccttg tacaagccat caaggaggca aaggagcagc
541 acccagacat gtcagtgacc aaggtggtcg tcaaccagga gaccgagatt gctgatgagt
601 gagctcagga actaacctac cccaactctg cccttctccc atccaagaga aaccagcaaa
661 atgataaaga agctaacctg ccatagtcag acttcagact ttcaagatta ttctaaatca
721 ccagaaaatt aatttcagtt tctattggga gtttatacca agagattctt ctagatctca
781 ttgatccttt tgaagagctt tttctatatt aggatatcag aattgttcaa cttttcactc
841 tatagactgt tttaagagtt ttggggtttt tttaattggg tggtttgtaa ccccttcagc
901 ctagcctctc tgcccattta tttccaaccc caacagacac tgacagggtc catggaattc
961 ttcgggaaat cctccaagga ctcttgtcag ctgtgttgga agccaaagcc agcttagtgg
1021 gacttccgcg tctctcccta gtcttatccc ctttggatga tggcagaaac ttcatgaacc
1081 agccctttct cagagccagt gatgtgagtg tatcagaatg ccagggaggg caccagccct
1141 gatccacaga cctcggaaag atgcccctgt tcctttgttg cgggtggttt tggtaaggca
1201 gagccctctg ctgagaatgt agtattg
//