LOCUS AK299069 2009 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58345 complete cds, highly similar to Zinc
finger protein 548.
ACCESSION AK299069
VERSION AK299069.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2009)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2009
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3004328"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493693"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 98..1735
/codon_start=1
/note="highly similar to Zinc finger protein 548"
/protein_id="BAG61135.1"
/transl_table=1
/translation="MNLTEGPLAMAEMDPTQGRVVFEDVAIYFSQEEWGHLDEAQRLL
YRDVMLENLALLSSLGSWHGAEDEEAPSQQGFSVGVSEVTTSKPCLSSQKVHPSETCG
PPLKDILCLVEHNGIHPEQHIYICEAELFQHPKQQIGENLSRGDDWIPSFGKNHRVHM
AEEIFTCMEGWKDLPATSCLLQHQGPQSEWKPYRDTEDREAFQTGQNDYKCSECGKTF
TCSYSFVEHQKIHTGERSYECNKCGKFFKYSANFMKHQTVHTSERTYECRECGKSFMY
NYRLMRHKRVHTGERPYECNTCGKFFRYSSTFVRHQRVHTGERPYECRECGKFFMDSS
TLIKHQRVHTGERPYKCNDCGKFFRYISTLIRHQRIHTGERPYECSVCGELFRYNSSL
VKHWRNHTGERPYKCSECGKSFRYHCRLIRHQRVHTGERPYECSECGKFFRYNSNLIK
HWRNHTGERPYECRECGKAFSHKHILVEHQKIHSGERPYECSECQKAFIRKSHLVHHQ
KIHSEERLVCSMNVGNSLAKTPTSLNIRDFTMEKVYH"
BASE COUNT 595 a 426 c 471 g 517 t
ORIGIN
1 gagtcaactg acaagcgctg gggacagtgg cgtccttgtc ttgcctttgt cgctcccgcc
61 ccgctcttcc ctggctgggc tggcggaggc cttgctgatg aacctgactg agggtcccct
121 ggcgatggca gaaatggacc ctacacaggg ccgtgtggtc tttgaggacg tggccatata
181 tttctcccag gaggagtggg ggcaccttga tgaggctcag agattgctgt accgtgatgt
241 gatgctggag aatttggccc ttttgtcctc actaggttct tggcatggag ctgaggatga
301 ggaggcacct tcacagcaag gtttttctgt aggagtgtca gaggttacaa cttcaaagcc
361 ctgtctgtcc agccagaagg tccaccctag tgagacatgt ggcccaccct tgaaagacat
421 tctgtgcctg gttgagcaca atggaattca tcctgagcaa cacatatata tttgtgaggc
481 agagcttttt cagcacccaa agcagcaaat tggagaaaat ctttccagag gggatgattg
541 gataccttca tttgggaaga accacagagt tcacatggca gaggagatct tcacatgcat
601 ggagggctgg aaggacttac cagccacctc atgccttctc cagcaccagg gccctcaaag
661 cgagtggaag ccatacaggg acacagagga cagagaagcc tttcagactg gacaaaatga
721 ttacaaatgt agtgaatgtg ggaaaacctt cacctgcagc tattcatttg ttgagcacca
781 gaaaatccac acaggagaaa ggtcttatga atgtaacaaa tgtgggaaat tctttaagta
841 cagtgccaat ttcatgaaac atcagacagt tcacactagt gaaaggactt atgagtgcag
901 agaatgtgga aaatccttta tgtacaacta ccgactcatg agacataagc gagttcacac
961 tggagaaagg ccttatgagt gcaacacatg tgggaaattc tttcggtaca gctccacatt
1021 tgttagacat cagagagttc acaccggaga aaggccgtat gagtgcaggg aatgtgggaa
1081 attctttatg gacagctcca cactcattaa acatcagaga gttcacaccg gagaaagacc
1141 ttataagtgc aatgattgtg ggaaattttt taggtatatc tccacactca ttagacatca
1201 gagaattcac actggagaaa ggccttatga gtgcagtgta tgtggggaat tgtttaggta
1261 caactccagc cttgttaaac attggagaaa tcacactgga gaaaggcctt ataaatgcag
1321 tgaatgtggg aaatcattta ggtaccactg caggctcatt agacaccaga gagtccacac
1381 gggagaaagg ccttatgagt gcagcgaatg cgggaaattc tttcgttaca actccaacct
1441 cattaaacat tggagaaatc acactggaga aaggccttac gagtgcagag agtgtgggaa
1501 agcctttagc cacaagcata tacttgttga gcaccagaaa atccacagtg gagaaagacc
1561 ttatgagtgc agcgaatgcc agaaggcctt tattagaaag tctcacctgg ttcatcacca
1621 gaaaatccac agtgaagaga ggcttgtgtg ctccatgaat gtggggaatt ctttagctaa
1681 aactccaacc tcattaaaca tcagagattt cacaatggag aaagtttacc attgactatt
1741 gtaattgggt agtaatgtta tataaattcc acatttttat gcaactaatc tccagaacat
1801 ttttcctctt accaagaagt aaaatgctgt acccattaac aacaactcat tccccttccc
1861 tacttcccca gaaatgtctc aactatattt ctatactcta tggtacttat atgaggtacc
1921 aatagatatc tatgaatttg atatatattt gtacctcata taagtggatt ctacagtatt
1981 tatcttttga gactggctta tttcactta
//