LOCUS AK299055 3581 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ54454 complete cds, highly similar to Homo
sapiens transmembrane channel-like 5 (TMC5), mRNA.
ACCESSION AK299055
VERSION AK299055.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3581)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3581
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3002756"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493679"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 543..3407
/codon_start=1
/note="highly similar to Homo sapiens transmembrane
channel-like 5 (TMC5), mRNA"
/protein_id="BAH12939.1"
/transl_table=1
/translation="MSAYYRNNWSEEDPDYPDYSGSQNRTQGYLKTQGYPDVPGPLNN
PDYPGTRSNPYSVASRTRPDYPGSLAEPNYPRSLSNPDYSGTRSNAYSAASRTSPDHP
TSLPEPDYSEFQSHPYHRASSRQPDYPGSQRNPDFAGSSSSGNYAGSRTHPDHFGSLE
PDYPGAQSNSDHPGPRANLNHPGSRKNLEHTSFRINPYADSLGKPDYPGADIQPNSPP
FFGEPDYPSAEDNQNLPSTWREPDYSDAENGHDYGSSETPKMTRGVLSRTSSIQPSFR
HRSDDPVGILWGENDYPEGIEMASMEMANSYGHSLPGAPGSGYVNPAYVGESGPVHAY
GNPPLSECDWHKSPQGQKLIASLIPMTSRDRIKAIRNQPRTMEEKRNLRKIVDKEKSK
QTHRILQLNCCIQCLNSISRAYRRSKNSLSEILNSISLWQKTLKIIGGKFGTSVLSYF
NFLRWLLKFNIFSFILNFSFIIIPQFTVAKKNTLQFTGLEFFTGVGYFRDTVMYYGFY
TNSTIQHGNSGASYNMQLAYIFTIGACLTTCFFSLLFSMAKYFRNNFINPHIYSGGIT
KLIFCWDFTVTHEKAVKLKQKNLSTEIRFLKTHSNPGAVLLLPFVVSCINLAVPCIYS
MFRLVERYEMPRHEVYVLLIRNIFLKISIIGILCYYWLNTVALSGEECWETLIGQDIY
RLLLMDFVFSLVNSFLGEFLRRIIGMQLITSLGLQEFDIARNVLELIYAQTLVWIGIF
FCPLLPFIQMIMLFIMFYSKNISLMMNFQPPSKAWRASQMMTFFIFLLFFPSFTGVLC
TLAITIWRLKPSADCGPFRGLPLFIHSIYSWIDTLSTRPGYLWVVWIYRNLIGSVHFF
FILTLIVLIITYLYWQITEGRKIMIRLLHEQIINEGKDKMFLIEKLIKLQDMEKKANP
SSLVLERREVEQQGFLHLGEHDGSLDLRSRRSVQEGNPRA"
BASE COUNT 985 a 907 c 813 g 876 t
ORIGIN
1 aggtaaggct gggtcatgga aagaaagaat gaatgcagac tacgggcggt gaatagaggt
61 ctcggatgcc agcaggcgtc ccaacccagc aggaactggc tcaattctca gaagaaagcg
121 atcggccccg aggcaggaag gccggctccg atgcagggcg cgccgcctgc gggctgcttc
181 gggccagggt cgacccgagg gccagcgcaa gcagcggcaa caggagcgcc aggaggacat
241 gaggctctgc ctgcagtcag caacttggaa tattcagact tcagaccagc atcacagatt
301 ataaccctcc gtaaatcatc tgcatcccag ctcccatcaa aagccagcct gaaggaccca
361 tggacacgtg actccagtgt tctcaacaac atcttaagat caagttggtt tgcacaacat
421 ttgcatctac ttgggacaaa gcaagaacaa taagggcaaa aaagtgaaaa aaaaaaaaga
481 tccctgagta attgcaaatg ctgggacagt ttaccactcc agggtgaaga gtccatacca
541 acatgtctgc ctactacagg aataactggt ctgaggaaga cccagattac cctgactatt
601 cagggtctca gaaccgtacg caggggtatt tgaaaactca aggttatcca gatgttccag
661 gtcctctgaa caatccagac taccccggca ccaggagcaa tccatactct gtagcctcca
721 gaacacgtcc agactatcct gggtctctgg cagaaccaaa ttatcctaga tctctgagta
781 atccagacta ttctggcacc agaagcaatg catactctgc agcctctaga acaagcccag
841 accatcctac ctctctacca gagccagatt atagtgaatt tcagagtcat ccctaccacc
901 gagcatcatc cagacaacca gactaccctg gatctcaacg aaatcctgat tttgcaggct
961 ccagcagcag tggaaactat gcaggctcca gaacacatcc agatcatttt ggctccttag
1021 aaccggacta ccctggagct cagagcaact ctgatcatcc tggacccaga gccaatttga
1081 accatccagg atccagaaag aatctggaac atacaagttt tagaatcaat ccatacgcag
1141 actctctggg aaagcctgat tatccaggcg ctgacattca acctaactct ccaccctttt
1201 ttggggagcc agactatccc agtgctgagg acaatcagaa cttgccaagc acttggagag
1261 aacctgatta ttcagatgct gagaatggtc atgattatgg ctcttctgag accccaaaga
1321 tgaccagggg ggtgctcagc agaacatctt caatccagcc ctcatttcgt cacaggagtg
1381 atgaccccgt gggcattctt tggggagaga atgattaccc tgaaggcatt gaaatggcat
1441 ccatggagat ggcaaactca tatggccact ctctgccagg tgctcctgga agtggctatg
1501 tgaaccctgc ttatgtaggt gaaagtggtc ctgtccatgc ttatggaaac ccaccattgt
1561 ctgaatgtga ttggcacaag tcaccccaag gacagaagtt aatcgcatcc cttataccca
1621 tgacatccag agacagaatt aaagccatca ggaaccagcc aaggaccatg gaagagaaaa
1681 ggaaccttag gaaaatagtt gacaaagaaa aaagcaaaca gacccatcgt atccttcagc
1741 tcaattgctg tattcagtgt ctgaactcca tttcccgggc ttatcggaga tccaagaaca
1801 gcctgtcgga aattctgaat tccatcagcc tgtggcagaa gacgctgaag atcattggag
1861 gcaagtttgg aaccagcgtc ctctcctatt tcaactttct gagatggctt ttgaagttca
1921 acattttctc attcatcctg aacttcagct tcatcataat ccctcagttt accgtggcca
1981 aaaagaacac cctccagttc actgggctgg agtttttcac tggggtgggt tattttaggg
2041 acacagtgat gtactatggc ttttacacca attccaccat ccagcacggg aacagcgggg
2101 catcctacaa catgcagctg gcctacatct tcacaatcgg agcatgcttg accacctgct
2161 tcttcagttt gctgttcagc atggccaagt atttccggaa caacttcatt aatccccaca
2221 tttactccgg agggatcacc aagctgatct tttgctggga cttcactgtc actcatgaaa
2281 aagctgtgaa gctaaaacag aagaatctta gcactgagat aaggttcctg aagacacaca
2341 gtaaccctgg ggcggtgctg ttactgcctt tcgttgtgtc ctgcattaat ctggccgtgc
2401 catgcatcta ctccatgttc aggcttgtgg agaggtacga gatgccacgg cacgaagtct
2461 acgttctcct gatccgaaac atctttttga aaatatcaat cattggcatt ctttgttact
2521 attggctcaa caccgtggcc ctgtctggtg aagagtgttg ggaaaccctc attggccagg
2581 acatctaccg gctccttctg atggattttg tgttctcttt agtcaattcc ttcctggggg
2641 agtttctgag gagaatcatt gggatgcaac tgatcacaag tcttggcctt caggagtttg
2701 acattgccag gaacgttcta gaactgatct atgcacaaac tctggtgtgg attggcatct
2761 tcttctgccc cctgctgccc tttatccaaa tgattatgct tttcatcatg ttctactcca
2821 aaaatatcag cctgatgatg aatttccagc ctccgagcaa agcctggcgg gcctcacaga
2881 tgatgacttt cttcatcttc ttgctctttt tcccatcctt caccggggtc ttgtgcaccc
2941 tggccatcac catctggaga ttgaagcctt cagctgactg tggccctttt cgaggtctgc
3001 ctctcttcat tcactccatc tacagctgga tcgacaccct aagtacacgg cctggctacc
3061 tgtgggttgt ttggatctat cggaacctca ttggaagtgt gcacttcttt ttcatcctca
3121 ccctcattgt gctaatcatc acctatcttt actggcagat cacagaggga aggaagatta
3181 tgataaggct gctccatgag cagatcatta atgagggcaa agataaaatg ttcctgatag
3241 aaaaattgat caagctgcag gatatggaga agaaagcaaa ccccagctca cttgttctgg
3301 aaaggagaga ggtggagcaa caaggctttt tgcatttggg ggaacatgat ggcagtcttg
3361 acttgcgatc tagaagatca gttcaagaag gtaatccaag ggcctgatga ctcttttggt
3421 aacgagacac caatcaaata aggggaggag atgaaaatgg aatgatttct tccatgccac
3481 ctgtgccttt aggaactgcc cagaagaaaa tccaaggctt tagccaggag cggaaactga
3541 ctaccatgta attatcaaag taaaattggg cattccatgc t
//