LOCUS AK299054 2631 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ54289 complete cds, highly similar to Neural
cell adhesion molecule 2 precursor.
ACCESSION AK299054
VERSION AK299054.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2631)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2631
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3002750"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493678"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 189..2276
/codon_start=1
/note="highly similar to Neural cell adhesion molecule 2
precursor"
/protein_id="BAH12938.1"
/transl_table=1
/translation="MSLLLSFYLLGLLVSSGQDRFAMLANNNLQILNINKSDEGIYRC
EGRVEARGEIDFRDIIVIVNVPPAISMPQKSFNATAERGEEMTFSCRASGSPEPAISW
FRNGKLIEENEKYILKGSNTELTVRNIINSDGGPYVCRATNKAGEDEKQAFLQVFVQP
HIIQLKNETTYENGQVTLVCDAEGEPIPEITWKRAVDGFTFTEGDKSPDGRIEVKGQH
GSSSLHIKDVKLSDSGRYDCEAASRIGGHQKSMYLDIEYAPKFISNQTIYYSWEGNPI
NISCDVKSNPPASIHWRRDKLVLPAKNTTNLKTYSTGRKMILEIAPTSDNDFGRYNCT
ATNHIGTRFQEYILALADVPSSPYGVKIIELSQTTAKVSFNKPDSHGGVPIHHYQVDV
KEVASEIWKIVRSHGVQTMVVLNNLEPNTTYEIRAAAVNGKGQGDYSKIEIFQTLPVR
EPSPPSIHGQPSSGKSFKLSITKQDDGGAPILEYIVKYRSKDKEDQWLEKKVQGNKDH
IILEHLQWTMGYEVQITAANRLGYSEPTVYEFSMPPKPNIIKDTLFNGLGLGAVIGLG
VAALLLILVVTDVSCFFIRQCGLLMCITRRMCGKKSGSSGKSKELEEGKAAYLKDGSK
EPIVEMRTEDERVTNHEDGSPVNEPNETTPLTEPEKLPLKEEDGKEALNPETIEIKVS
NDIIQSKEDDSKA"
BASE COUNT 861 a 515 c 616 g 639 t
ORIGIN
1 ggatatagtg cggcaagagc ggagcttgca gtcactttgc gaggaggagc gcgcgggctg
61 cgggcggctg gggcaccgcg ggagcggcgg cggcggctct agcagaggcg gccggggcag
121 cgaaaggttc tctctccagg gctggactta ataactttga aactgtccac cggtgtcacg
181 tcctgaacat gagcctcctc ctctccttct acctgctggg gttgcttgtc agtagcgggc
241 aagatcggtt cgctatgtta gcaaacaata acctgcagat tctcaacatc aataaaagtg
301 atgaaggtat atacagatgt gaaggaagag tggaggccag gggagaaatt gacttccgtg
361 atatcattgt tattgttaat gtgccgccag caatctcaat gcctcagaaa tcttttaatg
421 ccacagcaga gagaggagaa gaaatgacat tttcctgcag ggcctcaggc tctccagaac
481 ccgccatctc ctggttcagg aatggcaagc tcattgaaga aaatgagaag tacatattga
541 aagggagcaa tacagaactc actgtcagga acataatcaa tagtgatggt ggtccttatg
601 tctgcagggc cacaaataag gcaggagaag atgaaaagca agctttcctc caagtctttg
661 tacagcctca cataatacag cttaaaaatg aaactacata tgagaatggt caagtcacac
721 tcgtatgtga tgcggaaggg gagcctattc cagaaatcac ttggaaaaga gctgtggatg
781 gcttcacgtt cactgaaggc gataagagcc cggacggccg tatcgaagtc aaagggcagc
841 atggaagctc atcactgcat attaaagatg tgaagttgtc agattcaggg agatatgact
901 gtgaagctgc aagcagaatt ggagggcatc aaaagagcat gtaccttgat attgaatatg
961 cccccaagtt tatatcaaac caaacaattt attactcttg ggaaggaaat cctatcaata
1021 taagttgtga tgtgaaatcg aatccaccag catcaattca ctggagaaga gataaattag
1081 tcttacctgc taaaaacacg accaatttaa agacttatag tacaggaaga aagatgatat
1141 tagagattgc acctacatct gacaatgact ttggacgcta taattgcaca gccactaatc
1201 atataggaac aagatttcaa gaatatattc ttgctttggc tgacgtgcca tccagtccct
1261 atggagtgaa gatcatagag ctgtcgcaga ccacggccaa ggtttccttc aacaaaccgg
1321 actcccatgg aggtgtacct attcatcact atcaggtgga tgtcaaagaa gtagcgtcag
1381 aaatctggaa aattgtacgc tcccatggag ttcaaacaat ggttgttttg aacaacctgg
1441 aaccaaatac aacttatgaa atcagggctg cagctgtaaa tggaaaggga caaggagact
1501 acagtaaaat agaaatcttc caaacattac cagttcgtga accaagtcct ccatccatac
1561 atggacagcc aagcagtgga aagagcttta aactcagcat caccaaacag gacgatggag
1621 gggcccctat tttggaatac attgtgaaat atagaagtaa agataaggaa gaccaatggc
1681 tagagaaaaa agtgcaagga aataaagacc acatcatttt ggagcatctc cagtggacca
1741 tggggtatga agttcagatt acagctgcca atagattggg atattctgaa ccgacagttt
1801 atgaattcag catgccacca aagcccaaca ttattaaaga cacgctgttt aatggtcttg
1861 ggcttggagc agtaattggc ctgggagttg ctgcactgct gctaattctt gtggtaacag
1921 acgtcagctg cttctttatt cggcaatgtg ggttgctgat gtgcatcact aggagaatgt
1981 gtggaaagaa aagtggctcc agtggcaaaa gtaaagaact cgaagaagga aaagctgcat
2041 acctgaaaga tggatcaaaa gaaccaatag tggagatgag aacagaggat gaaagagtta
2101 ctaatcacga agatgggagc ccagtaaatg agccaaatga aaccacacca ctgacagaac
2161 ctgaaaaatt gcctttaaag gaagaagatg ggaaagaagc tctaaatcca gaaactatag
2221 aaattaaagt ttctaacgac atcattcaat caaaagaaga cgacagcaaa gcataacaac
2281 aatattacag gggcttgaac aacactacga agagtatttg gattgcgtga ccctatgacc
2341 aaaactattc cattgacctt aatttcttgg gaaacttcta gcttggaata gcttgtacac
2401 atatacatat gatcaaatac tcctgcccat gatccattcc cttttgttat tgttgttgtt
2461 gttgctgttg ttgttaattt tgttaagaat ttcaatatca agactgactg gcaccaacac
2521 tttggtattc aatttgattc tatgactgaa gtactggaat ttattatgtg gctaaagtgc
2581 tctatttatt aagaactata tttaatacca ccaacaaata taggggttaa g
//