LOCUS AK299042 3543 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55911 complete cds, highly similar to Solute
carrier family 12 member 4.
ACCESSION AK299042
VERSION AK299042.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3543)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3543
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3001805"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493666"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 21..3185
/codon_start=1
/note="highly similar to Solute carrier family 12 member
4"
/protein_id="BAG61116.1"
/transl_table=1
/translation="MGDTLSPGHGDHRESSPFLSPLEASRGIDYYDRNLALFEEELDI
RPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIF
GVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISR
SLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGAHDTSNATLN
NMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPV
CMLGNRTLSRDQFDICAKTAVVDNETVATQLWSFFCHSPNLTTDSCDPYFMLNNVTEI
PGIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSFTVL
VGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVV
LRDKYGDGVSRNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNII
PFLRVFGHGKVNGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACA
VQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQ
GAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLT
FASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVR
EGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPK
NIAFYPSNHERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDN
SIQMKKDLAVFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTE
REREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMTWTRDKYMTETWDPSHAPDNF
RELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDENYMEFLE
VLTEGLERVLLVRGGGREVITIYS"
BASE COUNT 679 a 1041 c 1045 g 778 t
ORIGIN
1 agaccttggc tgtctacagg atgggggaca ccctgagccc cggacatggc gaccacagag
61 agagcagccc ttttctttcc cccttggagg cttccagagg aattgactac tatgacagga
121 acctggcact gtttgaggaa gagctggaca tccgcccaaa ggtatcgtct cttctgggaa
181 agctcgtcag ctacaccaac ctcacccagg gcgccaaaga gcatgaggag gccgagagtg
241 gggagggcac ccgccggagg gcagccgagg cacccagcat gggcaccctc atgggggtgt
301 acctgccctg cctgcagaat atctttgggg ttatcctctt cctgcggctg acctggatgg
361 tgggcacagc aggtgtgcta caggccctcc tcatcgtgct tatctgctgc tgttgtaccc
421 tgctgacggc catctccatg agtgccatcg ccaccaacgg tgtggttcca gctgggggct
481 cctatttcat gatctctcgt tcactggggc cagaatttgg aggtgctgtg ggcctgtgct
541 tctacctggg aacaacattc gcagcagcca tgtacatcct gggggccatc gagatcttgc
601 tgacctacat tgccccacca gctgccattt tttacccatc gggtgctcat gacacgtcga
661 atgccacttt gaacaatatg cgtgtgtatg ggaccatttt cctgaccttc atgaccctgg
721 tggtgtttgt gggggtcaag tatgtgaaca aatttgcctc gctcttcctg gcctgtgtga
781 tcatctccat cctctccatc tatgctgggg gcataaagtc tatatttgac cctcccgtgt
841 ttccggtatg catgctgggc aacaggaccc tgtcccggga ccagtttgac atctgtgcca
901 agacagctgt agtggacaat gagacagtgg ccacccagct atggagtttc ttctgccaca
961 gccccaacct tacgaccgac tcctgtgacc cctacttcat gctcaacaat gtgaccgaga
1021 tccctggcat ccccggggca gctgctggtg tgctccagga aaacctgtgg agcgcctacc
1081 tggagaaggg tgacatcgtg gagaagcatg ggctgccctc cgcagatgcc ccgagcctga
1141 aggagagcct gcctctgtac gtggtcgctg acatcgccac atccttcacc gtgctggtcg
1201 gcatcttctt cccttctgta acaggcatca tggctggctc aaaccgctct ggggaccttc
1261 gtgacgccca gaagtctatc cctgtgggga ccattctggc catcattaca acttccctcg
1321 tgtacttcag cagtgtggtt ctctttggtg cctgcattga gggtgtggtt ctccgggaca
1381 agtatggcga tggtgtcagc aggaacttgg tggtgggcac actggcctgg ccttcaccct
1441 gggtcatcgt catcggctcc ttcttttcaa cgtgtggcgc tggcctccag agcctcacag
1501 gggcaccacg cctattgcag gccattgcca aggacaacat catccccttc ctccgggtgt
1561 ttggccacgg gaaggtgaat ggtgaaccca catgggcact cctcctgacg gcactcatcg
1621 ccgagctggg catcctcatc gcctccctcg acatggtggc ccccatctta tccatgttct
1681 ttctgatgtg ctacctgttc gtgaacctcg cctgtgcggt gcagacactc ctgaggaccc
1741 ccaactggcg gccccggttc aagtactatc actgggcgct gtccttcctg ggcatgagtc
1801 tctgcctggc ccttatgttt gtctcctcct ggtactatgc cctggtggcc atgctcatcg
1861 ccggcatgat ctacaaatac atcgagtacc aaggggctga gaaggagtgg ggtgacggga
1921 tccgaggcct gtccctgagc gctgcccgct acgcgctgtt gcggctggag gaggggcctc
1981 ctcacaccaa gaactggcgg ccgcagctgc tggtgctgct gaagctggac gaggacctcc
2041 acgtgaagta cccgcggctc ctcaccttcg cctcccagct caaggctggc aagggcctga
2101 ccattgttgg ttctgtcatc caggggagct tcttggagag ctatggcgag gctcaggccg
2161 ccgagcagac catcaagaac atgatggaaa ttgagaaggt gaagggcttc tgccaggtgg
2221 tggtggccag caaggtgcgg gaggggctgg cccacctcat ccagtcctgt ggcctgggag
2281 gcatgcggca taactccgtg gtgctgggct ggccctacgg ctggcgacag agcgaggacc
2341 cccgtgcctg gaagaccttc attgacaccg tgcgctgcac tacggctgcc cacctggccc
2401 tgctcgtgcc caagaacatc gccttctacc ccagcaacca cgagcgctac ctggagggcc
2461 acatagacgt gtggtggatc gtgcacgatg gtggcatgct catgcttctg cccttcctgc
2521 tgcgccagca taaggtctgg aggaagtgcc ggatgcgcat cttcacagtg gcccagatgg
2581 atgacaacag catccagatg aagaaggacc tggctgtctt tctgtaccat ctgcgccttg
2641 aggccgaggt ggaggtggtg gagatgcata acagtgacat ctctgcatac acctacgagc
2701 ggacgctgat gatggagcag cggtcgcaga tgctgcggca gatgagactg accaagactg
2761 agcgggagcg agaagcccag ctggtcaagg atcggcactc ggccctgcgg ctggagagcc
2821 tgtactcgga cgaggaagat gagtctgcag tgggggctga caagatccag atgacgtgga
2881 ccagggacaa gtacatgact gagacctggg accccagcca tgcccctgac aatttccggg
2941 agctggtgca cattaagccg gaccaatcca atgtgcggcg catgcacact gctgtgaagc
3001 tcaatgaagt cattgtcacg cgctcccacg acgcccgcct ggttctccta aacatgcctg
3061 gcccacccag gaacagtgag ggcgacgaga actacatgga gttcctcgag gtgctgaccg
3121 agggccttga gcgggtgctg ttggtgcgcg gtggtggccg tgaagtcatc accatctact
3181 cctgagccca gtgtcatctt gtggcctgga gtcgaggtct tggccaggac ataacaagct
3241 gtggtctggg gtaacagcct cttcccagca cccacctgcc agccctgctt gcctggccct
3301 gtcctggacc cagctttgct aggtctcctt ggaaaccagg cctgggcctc aaaatggaga
3361 tggatcccag gtcttgtggg accctgggat gtttggggac tttactatct agcaccccag
3421 taggcctgtc ctggccagag aagactggta ggggccgagt ggggtttgaa ggcagccggc
3481 ccggcccagc ccaggagcgc tatttattgc atatttattg tttggatgtc accatcagag
3541 acg
//