LOCUS AK299030 1845 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ55353 complete cds, highly similar to
Importin alpha-1 subunit.
ACCESSION AK299030
VERSION AK299030.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1845)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1845
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3000078"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493654"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 801..1814
/codon_start=1
/note="highly similar to Importin alpha-1 subunit"
/protein_id="BAG61106.1"
/transl_table=1
/translation="MCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSP
PPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVE
LLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWT
ISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYL
VELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEAKRNGTGINPYCALIEEAYG
LDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDLNQQQYIFQQCEAPMEG
FQL"
BASE COUNT 550 a 383 c 447 g 465 t
ORIGIN
1 gtctgagctc ttagaataac agagctcttc acagtaaact catttgttct ctccccactt
61 gtattgtaga aatcatgacc accccaggaa aagagaactt tcgcctgaaa agttacaaga
121 acaaatctct gaatcccgat gagatgcgca ggaggaggga ggaagaagga ctgcagttac
181 gaaagcagaa aagagaagag cagttattca agcggagaaa tgttgctaca gcagaagaag
241 aaacagaaga agaagttatg tcagatggag gctttcatga ggctcagatt aataacatgg
301 agatggcacc aggtggtgtc atcacttctg acatgattga aatgatattt tccaaaagcc
361 cagagcaaca gctttcagca acacagaaat tcaggaagct gctttcaaaa gaacctaacc
421 ctcctattga tgaagttatc agcacaccag gagtagtggc caggtttgtg gagttcctca
481 aacgaaaaga gaattgtaca ctgcaggtga gcctggcatt tgctaggaac cagagtggaa
541 ccggcaaagt ggccagcaac agagtcctga tccaaatgga gaaagggaac caggcgtacc
601 tcaacctgga gtgggaaact tgataggagt ttgaatcagc atgggtactg acaaatattg
661 cttcaggaaa ttctcttcag acccgaattg tgattcaggc aggagctgtg cccatcttca
721 tagagttgct cagctcagag tttgaagatg tccaggaaca ggcagtctgg gctcttggca
781 acattgctgg agatagtacc atgtgcaggg actatgtctt agactgcaat atccttcccc
841 ctcttttgca gttattttca aagcaaaacc gcctgaccat gacccggaat gcagtatggg
901 ctttgtctaa tctctgtaga gggaaaagtc cacctccaga atttgcaaag gtttctccat
961 gtctgaatgt gctttcctgg ttgctgtttg tcagtgacac tgatgtactg gctgatgcct
1021 gctgggccct ctcatatcta tcagatggac ccaatgataa aattcaagcg gtcatcgatg
1081 cgggagtatg taggagactt gtggaactgc tgatgcataa tgattataaa gtggtttctc
1141 ctgctttgcg agctgtggga aacattgtca caggggatga tattcagaca caggtaattc
1201 tgaattgctc agctctgcag agtttattgc atttgctgag tagcccaaag gaatctatca
1261 aaaaggaagc atgttggacg atatctaata ttacagctgg aaatagggca cagatccaga
1321 ctgtgataga tgccaacatt ttcccagccc tcattagtat tttacaaact gctgaatttc
1381 ggacaagaaa agaagcagct tgggccatca caaatgcaac ttctggagga tcagctgaac
1441 agatcaagta cctagtagaa ctgggttgta tcaagccgct ctgtgatctc ctcacggtca
1501 tggactctaa gattgtacag gttgccctaa atggcttgga aaatatcctg aggcttggag
1561 aacaggaagc caaaaggaat ggcactggca ttaaccctta ctgtgctttg attgaagaag
1621 cttatggtct ggataaaatt gagttcttac agagtcatga aaaccaggag atctaccaaa
1681 aggcctttga tcttattgag cattacttcg ggaccgaaga tgaagacagc agcattgcac
1741 cccaggttga ccttaaccag cagcagtaca tcttccaaca gtgtgaggct cctatggaag
1801 gtttccagct ttgaagcaat actctgcttt cacgttcctg tgtca
//